Search Results

Acetaldehyde accelerates HCV-induced impairment of innate immunity by suppressing methylation reactions in liver cells

Alcohol exposure worsens the course and outcomes of hepatitis C virus (HCV) infection. Activation of protective antiviral genes is induced by IFN-a signaling, which is altered in liver cells by either HCV or ethanol exposure. However, the mechanisms...

Authors: Murali Ganesan,1,2 Jinjin Zhang,3 Tatiana Bronich,3 Larisa I. Poluektova,2,4 Terrence M. Donohue,...

Quantitative Trait Locus Based Virulence Determinant Mapping of the HSV-1 Genome in Murine Ocular Infection: Genes Involved in Viral Regulatory and Innate Immune Networks Contribute to Virulence

Herpes simplex virus type 1 causes mucocutaneous lesions, and is the leading cause of infectious blindness in the United States. Animal studies have shown that the severity of HSV-1 ocular disease is influenced by three main factors; innate immunity,...

Authors: Kolb AW, Lee K, Larsen I, Craven M, Brandt CR

SST Peer Reviewed

Rhesus iPSC Safe Harbor Gene-Editing Platform for Stable Expression of Transgenes in Differentiated Cells of All Germ Layers.

Nonhuman primate (NHP) induced pluripotent stem cells (iPSCs) offer the opportunity to investigate the safety, feasibility, and efficacy of proposed iPSC-derived cellular delivery in clinically relevant in vivo models. However, there is need for...

Authors: Hong SG, Yada RC, Choi K, Carpentier A, Liang TJ, Merling RK, Sweeney CL, Malech HL, Jung M, Corat...

SST Peer Reviewed

3D Cultivation Techniques for Primary Human Hepatocytes.

One of the main challenges in drug development is the prediction of in vivo toxicity based on in vitro data. The standard cultivation system for primary human hepatocytes is based on monolayer cultures, even if it is known that these conditions...

Authors: Bachmann A1, Moll M2, Gottwald E3, Nies C4, Zantl R5, Wagner H6, Burkhardt B7, Sánchez JJ8, Ladurner...

Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation

Assisted reproductive technologies in all mammals are critically dependent on the quality of the oocytes used to produce embryos. For reasons not fully clear, oocytes matured in vitro tend to be much less competent to become fertilized, advance to...

Authors: Yuan Y, Spate LD, Redel BK, Tian Y, Zhou, Prather R, Roberts RM

Optogenetic stimulation of multiwell MEA plates for neural and cardiac applications

Microelectrode array (MEA) technology enables advanced drug screening and “disease-in-a-dish” modeling by measuring the electrical activity of cultured networks of neural or cardiac cells. Recent developments in human stem cell technologies,...

Authors: Clements IP, Millard DC, Nicolini AM, Preyer AJ, Grier R, Heckerling A, Blum RA, Tyler P, McSweeney...

SST Peer Reviewed

High-Throughput Platform for Identifying Molecular Factors Involved in Phenotypic Stabilization of Primary Human Hepatocytes In Vitro.

Liver disease is a leading cause of morbidity worldwide and treatment options are limited, with organ transplantation being the only form of definitive management. Cell-based therapies have long held promise as alternatives to whole-organ...

Authors: Shan J1, Logan DJ2, Root DE3, Carpenter AE3, Bhatia SN4

Precursor and mature NGF live tracking: one versus many at a time in the axons.

The classical view of nerve growth factor (NGF) action in the nervous system is linked to its retrograde axonal transport. However, almost nothing is known on the trafficking properties of its unprocessed precursor proNGF, characterized by different...

Authors: De Nadai T, Marchetti L, Di Rienzo C, Calvello M, Signore G3, Di Matteo P, Gobbo F, Turturro S,...

SST Peer Reviewed

Culture of bovine hepatocytes: a non-perfusion technique for cell isolation

In this work we have studied the isolation and culture of mature bovine hepatocytes on plastic dishes without exogenous matrix. The liver has been disaggregated in a collagenase solution instead of undergoing a perfusion step. After a few days in...

Authors: Spotorno VG1, Hidalgo A, Barbich M, Lorenti A, Zabal O.

Data on HO-1 and CD200 protein secretion during T-cells and mesenchymal stromal cells co-cultures.

In this Data in Brief, we have provided data describing the secretion profile of two main immunoregulatory proteins, heme oxygenase-1 (HO-1) and CD200, from bone marrow (BM), Wharton?s Jelly (WJ) or adipose tissue (AT) mesenchymal stromal cells...

Authors: Fayyad-Kazan M, Fayyad-Kazan H, Merimi M, Meuleman N, Bron D, Lagneaux L, Najar M

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Acetaldehyde accelerates HCV-induced impairment of innate immunity by suppressing methylation reactions in liver cells

Quantitative Trait Locus Based Virulence Determinant Mapping of the HSV-1 Genome in Murine Ocular Infection: Genes Involved in Viral Regulatory and Innate Immune Networks Contribute to Virulence

Rhesus iPSC Safe Harbor Gene-Editing Platform for Stable Expression of Transgenes in Differentiated Cells of All Germ Layers.

3D Cultivation Techniques for Primary Human Hepatocytes.

Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation

Optogenetic stimulation of multiwell MEA plates for neural and cardiac applications

High-Throughput Platform for Identifying Molecular Factors Involved in Phenotypic Stabilization of Primary Human Hepatocytes In Vitro.

Precursor and mature NGF live tracking: one versus many at a time in the axons.

Culture of bovine hepatocytes: a non-perfusion technique for cell isolation

Data on HO-1 and CD200 protein secretion during T-cells and mesenchymal stromal cells co-cultures.