Search Results

Endotoxin recovery using limulus amebocyte lysate (LAL) assay.

A phenomenon initially reported by Chen and Vinther in 2013 [1], and now commonly referred to as low endotoxin recovery (LER), has prompted the Food and Drug Administration (FDA) to request specific data demonstrating the capability of the LAL BET...

Authors: Bolden JS, Warburton RE, Phelan R, Murphy M, Smith KR, De Felippis MR, Chen D.

SST Peer Reviewed

Masking of endotoxin in surfactant samples: Effects on Limulus-based detection systems.

Over the last few decades Limulus Amebocyte Lysate (LAL) has been the most sensitive method for the detection of endotoxins (Lipopolysaccharides) and is well accepted in a broad field of applications. Recently, Low Endotoxin Recovery (LER) in...

Authors: Reich J1, Lang P2, Grallert H3, Motschmann H4

SST Peer Reviewed

Viability of primary cultured podocytes is associated with extracellular high glucose-dependent autophagy downregulation

Structural and functional impairment of podocytes plays an important role in the development of diabetic nephropathy, a chronic complication of diabetes mellitus and leading cause of renal failure requiring renal replacement therapy. Autophagy plays...

Authors: Audzeyenka I, Rogacka D, Piwkowska A, Angielski S, Jankowski M.

Gene knockdown studies revealed CCDC50 as a candidate gene in mantle cell lymphoma and chronic lymphocytic leukemia

The two B-cell non-Hodgkin's lymphoma entities, chronic lymphocytic leukemia (CLL) and mantle cell lymphoma (MCL), show recurrent chromosomal gains of 3q25-q29, 12q13-q14 and 18q21-q22. The pathomechanisms affected by these aberrations are not...

Authors: Farfsing A, Engel F, Seiffert M, Hartmann E, Ott G, Rosenwald A, Stilgenbauer S, Döhner H, Boutros...

SST Peer Reviewed

Nucleofection-based gene targeting in human pre-B cells.

Electroporation is a powerful and convenient means for transfection of nonviral vectors into mammalian cells, providing an essential tool for numerous applications including gene targeting via homologous recombination. Recent evidence clearly...

Authors: Kurosawa A, Saito S, Mori M, Adachi N.

Enhanced CAR T-cell engineering using non-viral Sleeping Beauty transposition from minicircle vectors.

Immunotherapy with T cell modified with gamma-retroviral or lentiviral (LV) vectors to express a chimeric antigen receptor (CAR) has shown remarkable efficacy in clinical trials. However, the potential for insertional mutagenesis and genotoxicity of...

Authors: Monjezi R1, Miskey C2, Gogishvili T1, Schleef M3, Schmeer M3, Einsele H1, Ivics Z2, Hudecek M1.

SST Peer Reviewed

Reprogramming Methods Do Not Affect Gene Expression Profile of Human Induced Pluripotent Stem Cells

Induced pluripotent stem cells (iPSCs) are pluripotent cells derived from adult somatic cells. After the pioneering work by Yamanaka, who first generated iPSCs by retroviral transduction of four reprogramming factors, several alternative methods to...

Authors: Marta Trevisan, Giovanna Desole, Giulia Costanzi, Enrico Lavezzo, Giorgio Palù, and Luisa Barzon*

SST Peer Reviewed

CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors.

The CRISPR/Cas9 system has recently been shown to facilitate high levels of precise genome editing using adeno-associated viral (AAV) vectors to serve as donor template DNA during homologous recombination (HR). However, the maximum AAV packaging...

Authors: Bak RO, Porteus MH.

SST Peer Reviewed

Reversal by ranolazine of doxorubicin-induced prolongation in the inactivation of late sodium current in rat dorsal root ganglion neurons

Authors: Wu SN, So EC, Liao YK, Huang YM

SST Peer Reviewed

A new approach to gene therapy using Sleeping Beauty to genetically modify clinical-grade T cells to target CD19

The advent of efficient approaches to the genetic modification of T cells has provided investigators with clinically appealing methods to improve the potency of tumor-specific clinical grade T cells. For example, gene therapy has been successfully...

Authors: Singh H Huls H Kebriaei P Cooper LJ

SST Peer Reviewed

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Endotoxin recovery using limulus amebocyte lysate (LAL) assay.

Masking of endotoxin in surfactant samples: Effects on Limulus-based detection systems.

Viability of primary cultured podocytes is associated with extracellular high glucose-dependent autophagy downregulation

Gene knockdown studies revealed CCDC50 as a candidate gene in mantle cell lymphoma and chronic lymphocytic leukemia

Nucleofection-based gene targeting in human pre-B cells.

Enhanced CAR T-cell engineering using non-viral Sleeping Beauty transposition from minicircle vectors.

Reprogramming Methods Do Not Affect Gene Expression Profile of Human Induced Pluripotent Stem Cells

CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors.

Reversal by ranolazine of doxorubicin-induced prolongation in the inactivation of late sodium current in rat dorsal root ganglion neurons

A new approach to gene therapy using Sleeping Beauty to genetically modify clinical-grade T cells to target CD19