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Downregulation of Organic Anion Transporting Polypeptide (OATP) 1B1 Transport Function by Lysosomotropic Drug Chloroquine: Implication in OATP-Mediated Drug-Drug Interactions

Organic anion transporting polypeptide (OATP) 1B1 mediates the hepatic uptake of many drugs including lipid-lowering statins. Decreased OATP1B1 transport activity is often associated with increased systemic exposure of statins and statin-induced...

Authors: Alam K, Pahwa S, Wang X, Zhang P, Ding K, Abuznait AH, Li L, Yue W

Targeted gene addition to a predetermined site in the human genome using a ZFN-based nicking enzyme

Zinc-finger nucleases (ZFNs) drive highly efficient genome editing by generating a site-specific DNA double-strand break (DSB) at a predetermined site in the genome. Subsequent repair of this break via the nonhomologous end-joining (NHEJ) or...

Authors: Jianbin Wang, Geoffrey Friedman, Yannick Doyon, Nathaniel S. Wang, Carrie Jiaxin Li, Jeffrey C....

SST Peer Reviewed

Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach

We developed an in vivo library-on-library methodology to simultaneously assess single guide RNA (sgRNA) activity across ~1,400 genomic loci. Assaying across multiple human cell types and end-processing enzymes as well as two Cas9 orthologs, we...

Authors: Raj Chari, Prashant Mali, Mark Moosburner, George M Church

Flow Cytometry Based Detection and Isolation of Plasmodium falciparum Liver Stages In Vitro

Malaria, the disease caused by Plasmodium parasites, remains a major global health burden. The liver stage of Plasmodium falciparum infection is a leading target for immunological and pharmacological interventions. Therefore, novel approaches...

Authors: Dumoulin PC, Trop SA, Ma J, Zhang H, Sherman MA, Levitskaya J

SST Peer Reviewed

CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruzi

Trypanosoma cruzi is a protozoan parasite of humans and animals, affecting 10 to 20 million people and innumerable animals, primarily in the Americas. Despite being the largest cause of infection-induced heart disease worldwide, even among the...

Authors: Duo Peng, Samarchith P. Kurup, Phil Y. Yao, Todd A. Minning, Rick L. Tarletona

SST Peer Reviewed

Efficient One-Step Production of Microencapsulated Hepatocyte Spheroids with Enhanced Functions

Hepatocyte spheroids microencapsulated in hydrogels can contribute to liver research in various capacities. The conventional approach of microencapsulating spheroids produces a variable number of spheroids per microgel and requires an extra step of...

Authors: Chan HF1,2, Zhang Y1,2, Leong KW1,2

Molecular beacon–enabled purification of living cells by targeting cell type–specific mRNAs

Molecular beacons (MBs) are dual-labeled oligonucleotides that fluoresce only in the presence of complementary mRNA. The use of MBs to target specific mRNAs allows sorting of specific cells from a mixed cell population. In contrast to existing...

Authors: Brian M Wile, Kiwon Ban, Young-Sup Yoon & Gang Bao

SST Peer Reviewed

T Cells Expressing CD19/CD20 Bispecific Chimeric Antigen Receptors Prevent Antigen Escape by Malignant B Cells.

The adoptive transfer of T cells expressing anti-CD19 chimeric antigen receptors (CARs) has shown remarkable curative potential against advanced B-cell malignancies, but multiple trials have also reported patient relapses due to the emergence of...

Authors: Zah E, Lin MY, Silva-Benedict A, Jensen MC, Chen YY

SST Peer Reviewed

Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells

The activities and genome-wide specificities of CRISPR-Cas Cpf1 nucleases are not well defined. We show that two Cpf1 nucleases from Acidaminococcus sp. BV3L6 and Lachnospiraceae bacterium ND2006 (AsCpf1 and LbCpf1, respectively) have on-target...

Authors: Benjamin P Kleinstiver, Shengdar Q Tsai, Michelle S Prew, Nhu T Nguyen, Moira M Welch, Jose M Lopez,...

SST Peer Reviewed

Long-term exposure to abnormal glucose levels alters drug metabolism pathways and insulin sensitivity in primary human hepatocytes.

Hyperglycemia in type 2 diabetes mellitus has been linked to non-alcoholic fatty liver disease, which can progress to inflammation, fibrosis/cirrhosis, and hepatocellular carcinoma. Understanding how chronic hyperglycemia affects primary human...

Authors: Davidson MD1,2, Ballinger KR3, Khetani SR1,2,3

Data Type

Category

Research Area

Platform

Topic

Species

Characteristic

Format

Origin

Downregulation of Organic Anion Transporting Polypeptide (OATP) 1B1 Transport Function by Lysosomotropic Drug Chloroquine: Implication in OATP-Mediated Drug-Drug Interactions

Targeted gene addition to a predetermined site in the human genome using a ZFN-based nicking enzyme

Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach

Flow Cytometry Based Detection and Isolation of Plasmodium falciparum Liver Stages In Vitro

CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruzi

Efficient One-Step Production of Microencapsulated Hepatocyte Spheroids with Enhanced Functions

Molecular beacon–enabled purification of living cells by targeting cell type–specific mRNAs

T Cells Expressing CD19/CD20 Bispecific Chimeric Antigen Receptors Prevent Antigen Escape by Malignant B Cells.

Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells

Long-term exposure to abnormal glucose levels alters drug metabolism pathways and insulin sensitivity in primary human hepatocytes.