Search Results

Survival, neuron-like differentiation and functionality of mesenchymal stem cells in neurotoxic environment: the critical role of erythropoietin.

Mesenchymal stem cells (MSCs) can ameliorate symptoms in several neurodegenerative diseases. However, the toxic environment of a degenerating central nervous system (CNS) characterized by hypoxia, glutamate (Glu) excess and amyloid beta (Abeta)...

Authors: Danielyan L, Schäfer R, Schulz A, Ladewig T, Lourhmati A, Buadze M, Schmitt AL, Verleysdonk S,...

SST Peer Reviewed

Spleen tyrosine kinase mediates BEAS-2B cell migration and proliferation and human rhinovirus-induced expression of vascular endothelial growth factor and interleukin-8.

Spleen tyrosine kinase (Syk) is an immunoregulatory tyrosine kinase that was identified originally in leukocytes. It is a key regulator of innate immunity as well as hematopoietic cell differentiation and proliferation. A role for Syk in regulating...

Authors: Xiaomin Wang, Mirek Mychajlowycz, Christine Lau, Carlos Gutierrez, Jeremy A. Scott, and Chung-Wai...

Rapid and direct microRNA quantification by an enzymatic luminescence assay

A quantitative bioluminescence assay for rapid and sensitive microRNA (miRNA) expression analysis was developed. The assay uses miRNA directly as a primer for binding to a circular single-stranded DNA template, followed by rolling circle...

Authors: Sun Y, Gregory KJ, Chen NG, Golovlev V

Evaluation of layers of the rat airway epithelial cell line RL-65 for permeability screening of inhaled drug candidates

A rat respiratory epithelial cell culture system for in vitro prediction of drug pulmonary absorption is currently lacking. Such a model may however enhance the understanding of interspecies differences in inhaled drug pharmacokinetics by filling the...

Authors: Hutter V, Hilgendorf C, Cooper A, Zann V, Pritchard DI, Bosquillon C.

Comparison of proliferative and multilineage differentiation potentials of cord matrix, cord blood, and bone marrow mesenchymal stem cells.

BACKGROUND: Hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs) are the two widely studied and characterized adult stem cells. Thus far, MSCs were obtained from the bone marrow, which is a painful procedure. Therefore, MSCs from...

Authors: Shetty P, Cooper K, Viswanathan C

SST Peer Reviewed

Molecular composition and ultrastructure of the caveolar coat complex

Caveolae are an abundant feature of the plasma membrane of many mammalian cell types, and have key roles in mechano-transduction, metabolic regulation, and vascular permeability. Caveolin and cavin proteins, as well as EHD2 and pacsin 2, are all...

Authors: Ludwig A, Howard G, Mendoza-Topaz C, Deerinck T, Mackey M, Sandin S, Ellisman MH, Nichols BJ

EF1alpha and RPL13a represent normalization genes suitable for RT-qPCR analysis of bone marrow derived mesenchymal stem cells

BACKGROUND: RT-qPCR analysis is a widely used method for the analysis of mRNA expression throughout the field of mesenchymal stromal cell (MSC) research. Comparison between MSC studies, both in vitro and in vivo, are challenging due to the varied...

Authors: Curtis KM, Gomez LA, Rios C, Garbayo E, Raval AP, Perez-Pinzon MA, Schiller PC

SST Peer Reviewed

The genetic and biochemical basis of FANCD2 monoubiquitination

Fanconi anaemia (FA) is a cancer predisposition syndrome characterized by cellular sensitivity to DNA interstrand crosslinkers. The molecular defect in FA is an impaired DNA repair pathway. The critical event in activating this pathway is...

Authors: Rajendra E, Oestergaard VH1, Langevin F1, Wang M1, Dornan GL, Patel KJ, Passmore LA

Bronchial epithelial cells are rendered insensitive to glucocorticoid transactivation by transforming growth factor-ß1.

BACKGROUND: We have previously shown that transforming growth factor-beta (TGF-beta) impairs glucocorticoid (GC) function in pulmonary epithelial cell-lines. However, the signalling cascade leading to this impairment is unknown. In the present...

Authors: Keenan CR, Mok JS, Harris T, Xia Y, Salem S, Stewart AG

The nitrated fatty acid 10-nitro-oleate attenuates allergic airway disease.

Asthma is a serious, growing problem worldwide. Inhaled steroids, the current standard therapy, are not always effective in this chronic inflammatory disease and can cause adverse effects. We tested the hypothesis that nitrated fatty acids (NFAs) may...

Authors: Reddy AT, Lakshmi SP, Dornadula S, Pinni S, Rampa DR, Reddy RC.

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Survival, neuron-like differentiation and functionality of mesenchymal stem cells in neurotoxic environment: the critical role of erythropoietin.

Spleen tyrosine kinase mediates BEAS-2B cell migration and proliferation and human rhinovirus-induced expression of vascular endothelial growth factor and interleukin-8.

Rapid and direct microRNA quantification by an enzymatic luminescence assay

Evaluation of layers of the rat airway epithelial cell line RL-65 for permeability screening of inhaled drug candidates

Comparison of proliferative and multilineage differentiation potentials of cord matrix, cord blood, and bone marrow mesenchymal stem cells.

Molecular composition and ultrastructure of the caveolar coat complex

EF1alpha and RPL13a represent normalization genes suitable for RT-qPCR analysis of bone marrow derived mesenchymal stem cells

The genetic and biochemical basis of FANCD2 monoubiquitination

Bronchial epithelial cells are rendered insensitive to glucocorticoid transactivation by transforming growth factor-ß1.

The nitrated fatty acid 10-nitro-oleate attenuates allergic airway disease.