Search Results

Dual biofunctional polymer modifications to address endothelialization and smooth muscle cell integration of ePTFE vascular grafts.

Expanded polytetrafluoroethylene (ePTFE) grafts were coated on the luminal surface with a cell-adhesive fluorosurfactant (FSP) polymer to promote endothelialization, followed by ethanol hydration to degas the pores and subsequent cell-adhesive,...

Authors: Bastijanic JM, Kligman FL, Marchant RE, Kottke-Marchant K

SST Peer Reviewed

Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells

Sequence-specific nucleases such as TALEN and the CRISPR/Cas9 system have so far been used to disrupt, correct or insert transgenes at precise locations in mammalian genomes. We demonstrate efficient ‘knock-in’ targeted replacement of multi-kilobase...

Authors: Susan M. Byrne, Luis Ortiz1, Prashant Mali, John Aach, George M. Church

EBV noncoding RNA binds nascent RNA to drive host PAX5 to viral DNA

EBER2 is an abundant nuclear noncoding RNA expressed by the Epstein-Barr virus (EBV). Probing its possible chromatin localization by CHART revealed EBER2's presence at the terminal repeats (TRs) of the latent EBV genome, overlapping previously...

Authors: Lee N, Moss WN, Yario TA, Steitz JA

SST Peer Reviewed

Engineering a 3D microfluidic culture platform for tumor-treating field application.

The limitations of current cancer therapies highlight the urgent need for a more effective therapeutic strategy. One promising approach uses an alternating electric field; however, the mechanisms involved in the disruption of the cancer cell cycle as...

Authors: Pavesi A, Adriani G, Tay A, Warkiani ME, Yeap WH5, Wong SC5, Kamm RD

SST Peer Reviewed

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system

Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P....

Authors: Mehdi Ghorbal, Molly Gorman, Cameron Ross Macpherson, Rafael Miyazawa Martins, Artur Scherf,...

SST Peer Reviewed

Site- and allele-specific polycomb dysregulation in T-cell leukaemia

T-cell acute lymphoblastic leukaemias (T-ALL) are aggressive malignant proliferations characterized by high relapse rates and great genetic heterogeneity. TAL1 is amongst the most frequently deregulated oncogenes. Yet, over half of the TAL1(+) cases...

Authors: Navarro JM1, Touzart A2, Pradel LC1, Loosveld M3, Koubi M4, Fenouil R1, Le Noir S2, Maqbool MA1,...

Angiogenic and anti-inflammatory properties of micro-fragmented fat tissue and its derived mesenchymal stromal cells.

BACKGROUND: Adipose-derived mesenchymal stromal cells (Ad-MSCs) are a promising tool for advanced cell-based therapies. They are routinely obtained enzymatically from fat lipoaspirate (LP) as SVF, and may undergo prolonged ex vivo expansion, with...

Authors: Ceserani V, Ferri A, Berenzi A, Benetti A, Ciusani E, Pascucci L, Bazzucchi C, Coccè V, Bonomi A,...

Designed transcription activator-like effector proteins efficiently induced the expression of latent HIV-1 in latently infected cells

HIV latency is the foremost barrier to clearing HIV infection from patients. Reactivation of latent HIV-1 represents a promising strategy to deplete these viral reservoirs. Here, we report a novel approach to reactivate latent HIV-1 provirus using...

Authors: Xiaohui Wang, Pengfei Wang, Zheng Fu, Haiyan Ji, Xiying Qu, Hanxian Zeng, Xiaoli Zhu, Junxiao Deng,...

Human lymphoblastoid B-cell lines reprogrammed to EBV-free induced pluripotent stem cells

Generation of patient-specific induced pluripotent cells (iPSCs) holds great promise for regenerative medicine. Epstein-Barr virus immortalized lymphoblastoid B-cell lines (LCLs) can be generated from a minimal amount of blood and are banked...

Authors: Deepika Rajesh, Sarah J. Dickerson, Junying Yu, Matthew E. Brown, James A. Thomson, and Nicholas J....

SST Peer Reviewed

3D Reconstruction of the Human Airway Mucosa In Vitro as an Experimental Model to Study NTHi Infections.

We have established an in vitro 3D system which recapitulates the human tracheo-bronchial mucosa comprehensive of the pseudostratified epithelium and the underlying stromal tissue. In particular, we reported that the mature model, entirely...

Authors: Marrazzo P, Maccari S, Taddei A, Bevan L, Telford J, Soriani M, Pezzicoli A

SST Peer Reviewed

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Dual biofunctional polymer modifications to address endothelialization and smooth muscle cell integration of ePTFE vascular grafts.

Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells

EBV noncoding RNA binds nascent RNA to drive host PAX5 to viral DNA

Engineering a 3D microfluidic culture platform for tumor-treating field application.

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system

Site- and allele-specific polycomb dysregulation in T-cell leukaemia

Angiogenic and anti-inflammatory properties of micro-fragmented fat tissue and its derived mesenchymal stromal cells.

Designed transcription activator-like effector proteins efficiently induced the expression of latent HIV-1 in latently infected cells

Human lymphoblastoid B-cell lines reprogrammed to EBV-free induced pluripotent stem cells

3D Reconstruction of the Human Airway Mucosa In Vitro as an Experimental Model to Study NTHi Infections.