Search Results

CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo.

Mammalian spermatogenesis is a highly regulated multistage process of sperm generation. It is hard to uncover the real function of a testis specific gene in vitro since the in vitro model is not yet mature. With the development of the CRISPR/Cas9...

Authors: Minyan Li, Rui Huang, Xue Jiang, Yuxi Chen, Zhen Zhang, Xiya Zhang, Puping Liang, Shaoquan Zhan,...

SST Peer Reviewed

Genetic modification of the diarrheal pathogen Cryptosporidium parvum

Recent studies into the global causes of severe diarrhoea in young children have identified the protozoan parasite Cryptosporidium as the second most important diarrhoeal pathogen after rotavirus. Diarrhoeal disease is estimated to be responsible for...

Authors: Vinayak S, Pawlowic MC, Sateriale A, Brooks CF, Studstill CJ, Bar-Peled Y, Cipriano MJ, Striepen B.

SST Peer Reviewed

SWI/SNF chromatin-remodeling complexes function in noncoding RNA-dependent assembly of nuclear bodies

Paraspeckles are subnuclear structures that form around nuclear paraspeckle assembly transcript 1 (NEAT1) long noncoding RNA (lncRNA). Recently, paraspeckles were shown to be functional nuclear bodies involved in stress responses and the development...

Authors: Kawaguchi T, Tanigawa A, Naganuma T, Ohkawa Y, Souquere S, Pierron G, Hirose T

Single-cell analyses of regulatory network perturbations using enhancer-targeting TALEs suggest novel roles for PU.1 during haematopoietic specification

Transcription factors (TFs) act within wider regulatory networks to control cell identity and fate. Numerous TFs, including Scl (Tal1) and PU.1 (Spi1), are known regulators of developmental and adult haematopoiesis, but how they act within wider TF...

Authors: Adam C. Wilkinson, Viviane K. S. Kawata, Judith Schütte, Xuefei Gao, Stella Antoniou, Claudia...

The Activation-Induced Assembly of an RNA/Protein Interactome Centered on the Splicing Factor U2AF2 Regulates Gene Expression in Human CD4 T Cells

Activation of CD4 T cells is a reaction to challenges such as microbial pathogens, cancer and toxins that defines adaptive immune responses. The roles of T cell receptor crosslinking, intracellular signaling, and transcription factor activation are...

Authors: Thomas C. Whisenant, Eigen R. Peralta, Lauren D. Aarreberg, Nina J. Gao, Steven R. Head, Phillip...

SST Peer Reviewed

Expression pattern and function of Notch2 in different subtypes of first trimester cytotrophoblast

INTRODUCTION: Notch signalling has been shown to control cytotrophoblast (CTB) proliferation, differentiation and motility suggesting that the conserved signalling pathway could be critical for human placental development. Since individual Notch...

Authors: Plessl K, Haider S, Fiala C, Pollheimer J, Knöfler M

SST Peer Reviewed

A platform for rapid generation of single and multiplexed reporters in human iPSC lines

Induced pluripotent stem cells (iPSC) are important tools for drug discovery assays and toxicology screens. In this manuscript, we design high efficiency TALEN and ZFN to target two safe harbor sites on chromosome 13 and 19 in a widely available and...

Authors: Pei Y, Sierra G, Sivapatham R, Swistowski A, Rao MS, Zeng X

Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs

Molecular beacons (MBs) are dual-labeled oligonucleotides that fluoresce only in the presence of complementary mRNA. The use of MBs to target specific mRNAs allows sorting of specific cells from a mixed cell population. In contrast to existing...

Authors: Brian M Wile, Kiwon Ban, Young-Sup Yoon, Gang Bao

SST Peer Reviewed

Epidermal growth factor receptor inhibition reduces angiogenesis via hypoxia-inducible factor-1a and Notch1 in head neck squamous cell carcinoma.

Angiogenesis, a marker of cancer development, affects response to radiotherapy sensibility. This preclinical study aims to understand the receptor tyrosine kinase-mediated angiogenesis in head neck squamous cell carcinoma (HNSCC). The receptor...

Authors: Wang WM, Zhao ZL, Ma SR, Yu GT, Liu B, Zhang L, Zhang WF, Kulkarni AB, Sun ZJ, Zhao YF

SST Peer Reviewed

A Single CRISPR-Cas9 Deletion Strategy that Targets the Majority of DMD Patients Restores Dystrophin Function in hiPSC-Derived Muscle Cells.

Mutations in DMD disrupt the reading frame, prevent dystrophin translation, and cause Duchenne muscular dystrophy (DMD). Here we describe a CRISPR/Cas9 platform applicable to 60% of DMD patient mutations. We applied the platform to DMD-derived hiPSCs...

Authors: Young CS, Hicks MR, Ermolova NV, Nakano H, Jan M, Younesi S, Karumbayaram S, Kumagai-Cresse C, Wang...

SST Peer Reviewed

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CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo.

Genetic modification of the diarrheal pathogen Cryptosporidium parvum

SWI/SNF chromatin-remodeling complexes function in noncoding RNA-dependent assembly of nuclear bodies

Single-cell analyses of regulatory network perturbations using enhancer-targeting TALEs suggest novel roles for PU.1 during haematopoietic specification

The Activation-Induced Assembly of an RNA/Protein Interactome Centered on the Splicing Factor U2AF2 Regulates Gene Expression in Human CD4 T Cells

Expression pattern and function of Notch2 in different subtypes of first trimester cytotrophoblast

A platform for rapid generation of single and multiplexed reporters in human iPSC lines

Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs

Epidermal growth factor receptor inhibition reduces angiogenesis via hypoxia-inducible factor-1a and Notch1 in head neck squamous cell carcinoma.

A Single CRISPR-Cas9 Deletion Strategy that Targets the Majority of DMD Patients Restores Dystrophin Function in hiPSC-Derived Muscle Cells.