Search Results

Modeling retinitis pigmentosa through patient-derived retinal organoids

Human-induced pluripotent stem cells (hiPSCs) can be differentiated into well-structured retinal organoids. In this protocol, we successfully established 3D retinae from patient-derived hiPSCs and built the retinitis pigmentosa model in vitro....

Authors: Yan-Ping Li , Wen-Li Deng , Zi-Bing Jin

SST Peer Reviewed

A functional genetic toolbox for human tissue-derived organoids

Human organoid systems recapitulate key features of organs offering platforms for modelling developmental biology and disease. Tissue-derived organoids have been widely used to study the impact of extrinsic niche factors on stem cells. However, they...

Authors: Dawei Sun , Lewis Evans , Francesca Perrone , Vanesa Sokleva , Kyungtae Lim , Saba Rezakhani ,...

Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4 + T cells allows complex functional analyses

CD4+ T cells are central mediators of adaptive and innate immune responses and constitute a major reservoir for human immunodeficiency virus (HIV) in vivo. Detailed investigations of resting human CD4+ T cells have been precluded by the absence of...

Authors: Manuel Albanese, Adrian Ruhle, Jennifer Mittermaier, Ernesto Mejías-Pérez, Madeleine Gapp, Andreas...

SST Peer Reviewed

CYP450 drug inducibility in NAFLD via an in vitro hepatic model:Understanding drug-drug interactions in the fatty liver

Drug-drug-interactions (DDIs) occur when a drug alters the metabolic rate, efficacy, and toxicity of concurrently used drugs. While almost 1 in 4 adults now use at least 3 concurrent prescription drugs in the United States, the Non-alcoholic fatty...

Authors: Camilo Rey-Bedon , Peony Banik , Aslihan Gokaltun, O Hofheinz , Martin L Yarmush , M Korkut...

In vitro and in vivo evaluation of discogenic cells, an investigational cell therapy for disc degeneration

BACKGROUND/CONTEXT: Disc degeneration (DD) is a significant driver of low back painand few treatments exist to treat the pain and disability associated with the disease.PURPOSE: Our group has developed a method to generate therapeutic discogenic...

Authors: Lara Ionescu Silverman, Galina Dulatova,Terry Tandeski, Isaac E. Erickson, Beverly Lundell, David...

Large-scale GMP-compliant CRISPR-Cas9–mediated deletion of the glucocorticoid receptor in multivirus-specific T cells

Virus-specific T cells have proven highly effective for the treatment of severe and drug-refractory infections after hematopoietic stem cell transplant (HSCT). However, the efficacy of these cells is hindered by the use of glucocorticoids, often...

Authors: Basar R, Daher M, Uprety N, Gokdemir E, Alsuliman A, Ensley E, Ozcan G, Mendt M, Hernandez Sanabria...

Sperm DNA damage causes genomic instability in early embryonic development

Genomic instability is common in human embryos, but the underlying causes are largely unknown. Here, weexamined the consequences of sperm DNA damage on the embryonic genome by single-cell whole-genomesequencing of individual blastomeres from bovine...

Authors: Sjors Middelkamp, Helena T. A. van Tol, Diana C. J. Spierings, Sander Boymans,Victor Guryev, Bernard...

Generation of hepatobiliary organoids from human induced pluripotent stem cells

To date, most of the pluripotent stem cell (PSC)-based liver modeling systems have initiated differentiation from highly purified definitive endoderm (DE) and have generated monolayer hepatocyte-like cells. However, preclinical predictions from such...

Authors: Fenfang Wu , Di Wu , Yong Ren , Yuhua Huang , Bo Feng , Nan Zhao , Taotao...

Functional Genomics: Arrayed CRISPR KO Screens

Functional genomics is the discipline in molecular and cellular biology field that studies how genes and pathways contribute to a particular disease phenotype. Functional genomic screens are key in target discovery and hold the promise to enable the...

Authors: Davide Gianni and Leire Escudero-Ibarz

SST Peer Reviewed

a2-HS Glycoprotein in Plasma Extracellular Vesicles Inhibits the Osteogenic Differentiation of Human Mesenchymal Stromal Cells In Vitro

Extracellular vesicles (Evs) contain diverse functional proteins, mRNAs, miRNAs, and DNA fragments, are secreted by various types of cells, and play important roles in cellular communication. Here, we show for the first time that plasma Evs inhibited...

Authors: Xiaohua Wu , Mengjun Ma , Peng Wang , Zhongyu Xie , Shan Wang , Hongjun Su , Wen Deng , Pei...

Data Type

Category

Research Area

Platform

Topic

Species

Characteristic

Format

Origin

Modeling retinitis pigmentosa through patient-derived retinal organoids

A functional genetic toolbox for human tissue-derived organoids

Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4 + T cells allows complex functional analyses

CYP450 drug inducibility in NAFLD via an in vitro hepatic model:Understanding drug-drug interactions in the fatty liver

In vitro and in vivo evaluation of discogenic cells, an investigational cell therapy for disc degeneration

Large-scale GMP-compliant CRISPR-Cas9–mediated deletion of the glucocorticoid receptor in multivirus-specific T cells

Sperm DNA damage causes genomic instability in early embryonic development

Generation of hepatobiliary organoids from human induced pluripotent stem cells

Functional Genomics: Arrayed CRISPR KO Screens

a2-HS Glycoprotein in Plasma Extracellular Vesicles Inhibits the Osteogenic Differentiation of Human Mesenchymal Stromal Cells In Vitro