Search Results

Increased yields and biological potency of knob into-hole-based soluble MHC class II molecules

Assembly of soluble peptide-major histocompatibility complex class II (pMHCII) monomersinto multimeric structures enables the detection of antigen-specific CD4+ T cells in biologicalsamples and, in some configurations, their reprogramming in vivo....

Authors: Pau Serra, Nahir Garabatos, Santiswarup Singha, César Fandos, Josep Garnica, Patricia Solé,Daniel...

RNAi Transfection Optimized in Primary Naïve B Cells for the Targeted Analysis of Human Plasma Cell Differentiation

Upon antigen recognition, naïve B cells undergo rapid proliferation followed by differentiation to specialized antibody secreting cells (ASCs), called plasma cells. Increased circulating plasma cells are reported in patients with B cell-associated...

Authors: Shih T, De S, Barnes BJ

High cell density transient transfection of CHO cellsfor TGF-beta1 expression

High cell densities for transient transfection with polyethyleneimine (PEI) can be usedfor rapid and maximal production of recombinant proteins. High cell densities can beobtained by different cultivation systems, such as batch or perfusion systems....

Authors: Abdalla A. Elshereef, André Jochums Antonina Lavrentieva,Lena Stuckenberg, Thomas Scheper Dörte...

Orthotopic T-cell receptor replacement in primary human T cells using CRISPR-Cas9-mediatedhomology-directed repair

Adoptive T cell therapy using T-cell receptor (TCR)-engineered T cells allows to redirect T cell specificity and to target any antigen of interest. Here, we apply advanced genetic engineering using clustered regularly interspaced short palindromic...

Authors: Carolin Moosmann,* Thomas R. Mueller, Dirk H. Busch, and Kilian Schober

High temporal resolution proteome and phosphoproteome profiling of stem cell-derived hepatocyte development

Primary human hepatocytes are widely used to evaluate liver toxicity of drugs, but they are scarce and demanding to culture. Stem cell-derived hepatocytes are increasingly discussed as alternatives. To obtain a better appreciation of the molecular...

Authors: Johannes Krumm, Keisuke Sekine , Patroklos Samaras, Agnieska Brazovskaja , Markus Breunig , Ryota...

Sialic Acid-Engineered IL4–10 Fusion Protein is Bioactiveand Rapidly Cleared from the Circulation

Purpose Modulating sialylation of therapeutic glycoproteinsmay be used to influence their clearance and systemic exposure.We studied the effect of low and high sialylated IL4–10fusion protein (IL4–10 FP) on in vitro and in vivo bioactivity...

Authors: Cristine Steen-Louws & Peter Boross1 & Judith Prado & Jan Meeldijk1 & Jurgen B. Langenhorst &Alwin...

Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies

Adoptive transfer of genetically modified immune cells holds great promise for cancer immunotherapy. CRISPR knockin targeting can improve cell therapies, but more high-throughput methods are needed to test which knockin gene constructs most potently...

Authors: Roth TL, Li PJ, Blaeschke F, Nies JF, Apathy R, Mowery C, Yu R, Nguyen MLT, Lee Y, Truong A, Hiatt...

SST Peer Reviewed

Targeted T cell receptor gene editing provides predictable T cell product function for immunotherapy

Adoptive transfer of T cells expressing a transgenic T cell receptor (TCR) has the potential to revolutionize immunotherapy of infectious diseases and cancer. However, the generation of defined TCR-transgenic T cell medicinal products with...

Authors: Thomas R Müller , Sebastian Jarosch 1, Monika Hammel , Justin Leube , Simon Grassmann , Bettina...

SST Peer Reviewed

Application of in- vitro-cultured primary hepatocytes to evaluate species translatability and AAV transduction mechanisms of action

Recombinant adeno-associated virus (AAV) is an effective platform for therapeutic gene transfer; however, tissue-tropism differences between species are a challenge for successful translation of preclinical results to humans. We evaluated the use of...

Authors: Su Liu , Lisa Razon , Olivia Ritchie , Choong-Ryoul Sihn , Britta Handyside , Geoffrey Berguig ,...

Candidate Markers for Stratification and Classification in Rheumatoid Arthritis

Rheumatoid arthritis (RA) is a chronic autoimmune, inflammatory disease, characterizedby synovitis in small- and medium-sized joints and, if not treated early and efficiently, jointdamage, and destruction. RA is a heterogeneous disease with a...

Authors: Lucius Bader, Stein-Erik Gullaksen, Nello Blaser, Morten Brun,Gerd Haga Bringeland, André Sulen,...

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