Search Results

Mincle Activation and the Syk/Card9 Signaling Axis are Central to the Development of the Autoimmune Disease of the Eye

Uveitis, which occurs in association with systemic immunological diseases, presents a considerable medical challenge because of incomplete understanding of its pathogenesis. The signals that initiate T cells to target the eye, which may be of...

Authors: Ellen J. Lee, Brieanna R. Brown, Emily E. Vance, Paige E. Snow, Phyllis B. Silver, David...

Establishment of Humanized Mice from Peripheral Blood Mononuclear Cells or Cord Blood CD34+ Hematopoietic Stem Cells for Immune-Oncology Studies Evaluating New Therapeutic Agents

The clinical success of immune checkpoint modulators and the development of next-generation immune-oncology (IO) agents underscore the need for robust preclinical models to evaluate novel IO therapeutics. Human immune system (HIS) mouse models enable...

Authors: Bhavna Verma , Amy Wesa

SST Peer Reviewed

Proteoglycan-Induced Arthritis and Recombinant Human Proteoglycan Aggrecan G1 Domain–Induced Arthritis in BALB/c Mice Resembling Two Subtypes of Rheumatoid Arthritis

Objective. To develop a simplified and relatively inexpensive version of cartilage proteoglycan–induced arthritis (PGIA), an autoimmunity model of rheumatoid arthritis (RA), and to evaluate the extent to which this new model replicates the disease...

Authors: Tibor T. Glant, Marianna Radacs, Gyorgy Nagyeri, Katalin Olasz, Anna Laszlo, Ferenc Boldizsar, Akos...

Genome-wide CRISPR Screening to Identify Drivers of TGF-ß-Induced Liver Fibrosis in Human Hepatic Stellate Cells

Liver fibrosis progression in chronic liver disease leads to cirrhosis, liver failure, or hepatocellular carcinoma and often ends in liver transplantation. Even with an increased understanding of liver fibrogenesis and many attempts to generate...

Authors: Shan Yu,*† Matthew Ericson,† Andrea Fanjul,† Derek M. Erion,‡ Maria Paraskevopoulou,‡ Erin N....

SST Peer Reviewed

Identification and Characterization of a TranscribedDistal Enhancer Involved in Cardiac Kcnh2Regulation

The human ether-a-go-go-related gene KCNH2encodes the voltage-gated potassium channelunderlying IKr, a current critical for the repolarizationphase of the cardiac action potential. Mutations inKCNH2 that cause a reduction of the repolarizingcurrent...

Authors: Malou van den Boogaard,Jan Hendrik van Weerd,Amira C. Bawazeer, ..., Phil Barnett,Jeroen Bakkers,...

Generation and maturation of bone marrow-derived DCs under serum-free conditions

Standard protocols for the generation of murine dendritic cells (DCs) employ medium supplemented with heat-inactivated fetalcalf serum (FCS). Recently, several attempts have been made to avoid serum exposure during DC culture. The impetus for...

Authors: Sung Jung Kim, Betty Diamond

Point-of-use, automated fabrication of a 3D human liver model supplemented with human adipose microvessels

Advanced in vitro tissue models better reflect healthy and disease tissue conditions in the body. However, complex tissue models are often manufactured using custom solutions and can be challenging to manufacture to scale. Here, we describe the...

Authors: Sarah M Moss , Jillian Schilp, Maya Yaakov , Madison Cook , Erik Schuschke , Brandon Hanke , Hannah...

CARAMBA: a first-in-human clinical trial with SLAMF7 CAR-T cells prepared by virus-free Sleeping Beauty gene transfer to treat multiple myeloma

Clinical development of chimeric antigen receptor (CAR)-T-cell therapy has been enabled by advances in synthetic biology, genetic engineering, clinical-grade manufacturing, and complex logistics to distribute the drug product to treatment sites. A...

Authors: Sabrina Prommersberger , Michael Reiser , Julia Beckmann , Sophia Danhof , Maximilian Amberger ,...

SST Peer Reviewed

POTENTIAL ROLE OF CULTURE MEDIUMS FOR SUCCESSFUL ISOLATION ANDNEURONAL DIFFERENTIATION OF AMNIOTIC FLUID STEM CELLS

In recent years, the use of stem cells has generated increasing interest in regenerative medicineand cancer therapies. The most potent stem cells derive from the inner cell mass during embryonicdevelopment and their use yields serious ethical and...

Authors: M. ORCIANI, M. EMANUELLII, C. MARTIN0, A. PUGNALONI,A.L. TRANQUILLI and R. DI PRIMIO

Tracking keratinocytes and melanocytes usingcarboxyfluorescein hydroxysuccinimidyl esterstaining

IntroductionThe treatment of burn wounds and hypopigmentation conditions often require autologoustransplantation of keratinocytes and melanocytes. Tracking transplanted cells to ascertaintheir contribution to tissue recapitulation presents a...

Authors: Susanna Lo¨ nnqvist, Johan P. E. JunkerID, Maria Sedell1, Erika Nyman,Gunnar KratzID

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Mincle Activation and the Syk/Card9 Signaling Axis are Central to the Development of the Autoimmune Disease of the Eye

Establishment of Humanized Mice from Peripheral Blood Mononuclear Cells or Cord Blood CD34+ Hematopoietic Stem Cells for Immune-Oncology Studies Evaluating New Therapeutic Agents

Proteoglycan-Induced Arthritis and Recombinant Human Proteoglycan Aggrecan G1 Domain–Induced Arthritis in BALB/c Mice Resembling Two Subtypes of Rheumatoid Arthritis

Genome-wide CRISPR Screening to Identify Drivers of TGF-ß-Induced Liver Fibrosis in Human Hepatic Stellate Cells

Identification and Characterization of a TranscribedDistal Enhancer Involved in Cardiac Kcnh2Regulation

Generation and maturation of bone marrow-derived DCs under serum-free conditions

Point-of-use, automated fabrication of a 3D human liver model supplemented with human adipose microvessels

CARAMBA: a first-in-human clinical trial with SLAMF7 CAR-T cells prepared by virus-free Sleeping Beauty gene transfer to treat multiple myeloma

POTENTIAL ROLE OF CULTURE MEDIUMS FOR SUCCESSFUL ISOLATION ANDNEURONAL DIFFERENTIATION OF AMNIOTIC FLUID STEM CELLS

Tracking keratinocytes and melanocytes usingcarboxyfluorescein hydroxysuccinimidyl esterstaining