Search Results

DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated Breaks.

The repair outcomes at site-specific DNA double-strand breaks (DSBs) generated by the RNA-guided DNA endonuclease Cas9 determine how gene function is altered. Despite the widespread adoption of CRISPR-Cas9 technology to induce DSBs for genome...

Authors: van Overbeek M, Capurso D, Carter MM, Thompson MS, Frias E, Russ C, Reece-Hoyes JS, Nye C, Gradia S,...

SST Peer Reviewed

Astrocyte elevated gene-1 is a novel modulator of HIV-1-associated neuroinflammation via regulation of nuclear factor-?B signaling and excitatory amino acid transporter-2 repression.

Astrocyte elevated gene-1 (AEG-1), a novel human immunodeficiency virus (HIV)-1 and tumor necrosis factor (TNF)-a-inducible oncogene, has generated significant interest in the field of cancer research as a therapeutic target for many metastatic...

Authors: Vartak-Sharma N, Gelman BB, Joshi C, Borgamann K, Ghorpade A

SST Peer Reviewed

Differential cell adhesion of breast cancer stem cells on biomaterial substrate with nanotopographical cues.

Cancer stem cells are speculated to have the capability of self-renewal and re-establishment of tumor heterogeneity, possibly involved in the potential relapse of cancer. CD44+CD24-/lowESA+ cells have been reported to possess tumorigenic properties,...

Authors: Tan KK, Giam CS, Leow MY, Chan CW, Yim EK1

Dengue virus sero-cross-reactivity drives antibody-dependent enhancement of infection with zika virus.

Zika virus (ZIKV) was discovered in 1947 and was thought to lead to relatively mild disease. The recent explosive outbreak of ZIKV in South America has led to widespread concern, with reports of neurological sequelae ranging from Guillain Barré...

Authors: Dejnirattisai W, Supasa P, Wongwiwat W, Rouvinski A, Barba-Spaeth G, Duangchinda T, Sakuntabhai A,...

Alterations in cholesterol metabolism restrict HIV-1 trans infection in nonprogressors

HIV-1-infected nonprogressors (NP) inhibit disease progression for years without antiretroviral therapy. Defining the mechanisms for this resistance to disease progression could be important in determining strategies for controlling HIV-1 infection....

Authors: Rappocciolo G, Jais M, Piazza P, Reinhart TA, Berendam SJ, Garcia-Exposito L, Gupta P, Rinaldo CR

SST Peer Reviewed

Bromodeoxyuridine does not contribute to sister chromatid exchange events in normal or Bloom syndrome cells.

Sister chromatid exchanges (SCEs) are considered sensitive indicators of genome instability. Detection of SCEs typically requires cells to incorporate bromodeoxyuridine (BrdU) during two rounds of DNA synthesis. Previous studies have suggested that...

Authors: van Wietmarschen N, Lansdorp PM

Cooperation between BRCA1 and vitamin D is critical for histone acetylation of the p21waf1 promoter and for growth inhibition of breast cancer cells and cancer stem-like cells.

Carriers of germline mutations in the BRCA1 gene have a significant increased lifetime risk for being diagnosed with breast cancer. The incomplete penetrance of BRCA1 suggests that environmental and/or genetic factors modify the risk and incidence...

Authors: Pickholtz I, Saadyan S, Keshet GI, Wang VS, Cohen R, Bouwman P, Jonkers J, Byers SW6, Papa MZ,...

Diverse epigenetic strategies interact to control epidermal differentiation.

It is becoming clear that interconnected functional gene networks, rather than individual genes, govern stem cell self-renewal and differentiation. To identify epigenetic factors that impact on human epidermal stem cells we performed siRNA-based...

Authors: Mulder KW, Wang X, Escriu C, Ito Y, Schwarz RF, Gillis J, Sirokmány G, Donati G, Uribe-Lewis S,...

SST Peer Reviewed

TP53 oncomorphic mutations predict resistance to platinum- and taxane-based standard chemotherapy in patients diagnosed with advanced serous ovarian carcinoma

Individual mutations in the tumor suppressor TP53 alter p53 protein function. Some mutations create a non-functional protein, whereas others confer oncogenic activity, which we term 'oncomorphic'. Since mutations in TP53 occur in nearly all ovarian...

Authors: Brachova P, Mueting SR, Carlson MJ1, Goodheart MJ, Button AM, Mott SL, Dai D, Thiel KW, Devor EJ,...

An optimized multiplex flow cytometry protocol for the analysis of intracellular signaling in peripheral blood mononuclear cells.

Phosphoflow cytometry is increasingly being used as a tool for the discovery of biomarkers used in the treatment and monitoring of disease and therapy. The ability to measure numerous phospho-protein targets simultaneously at a single cell level...

Authors: Davies R, Vogelsang P, Jonsson R, Appel S.

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DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated Breaks.

Astrocyte elevated gene-1 is a novel modulator of HIV-1-associated neuroinflammation via regulation of nuclear factor-?B signaling and excitatory amino acid transporter-2 repression.

Differential cell adhesion of breast cancer stem cells on biomaterial substrate with nanotopographical cues.

Dengue virus sero-cross-reactivity drives antibody-dependent enhancement of infection with zika virus.

Alterations in cholesterol metabolism restrict HIV-1 trans infection in nonprogressors

Bromodeoxyuridine does not contribute to sister chromatid exchange events in normal or Bloom syndrome cells.

Cooperation between BRCA1 and vitamin D is critical for histone acetylation of the p21waf1 promoter and for growth inhibition of breast cancer cells and cancer stem-like cells.

Diverse epigenetic strategies interact to control epidermal differentiation.

TP53 oncomorphic mutations predict resistance to platinum- and taxane-based standard chemotherapy in patients diagnosed with advanced serous ovarian carcinoma

An optimized multiplex flow cytometry protocol for the analysis of intracellular signaling in peripheral blood mononuclear cells.