Search Results

Modulation of the Surface Proteome through Multiple Ubiquitylation Pathways in African Trypanosomes.

Recently we identified multiple suramin-sensitivity genes with a genome wide screen in Trypanosoma brucei that includes the invariant surface glycoprotein ISG75, the adaptin-1 (AP-1) complex and two deubiquitylating enzymes (DUBs) orthologous to...

Authors: Zoltner M, Leung KF, Alsford S, Horn D, Field MC

SST Peer Reviewed

Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells

Sequence-specific nucleases such as TALEN and the CRISPR/Cas9 system have so far been used to disrupt, correct or insert transgenes at precise locations in mammalian genomes. We demonstrate efficient ‘knock-in’ targeted replacement of multi-kilobase...

Authors: Susan M. Byrne, Luis Ortiz1, Prashant Mali, John Aach, George M. Church

Regulation of the Flt3 Gene in Haematopoietic Stem and Early Progenitor Cells

The MYB transcription factor plays critical roles in normal and malignant haematopoiesis. We previously showed that MYB was a direct activator of FLT3 expression within the context of acute myeloid leukaemia. During normal haematopoiesis, increasing...

Authors: Giacomo Volpe, Mary Clarke, Paloma Garcìa, David Scott Walton, Alexandros Vegiopoulos, Walter Del...

SST Peer Reviewed

Current Protocols in Stem Cell Biology: CRISPR-Mediated Gene Targeting of Human Induced Pluripotent Stem Cells.

CRISPR/Cas9 nuclease systems can create double-stranded DNA breaks at specific sequences to efficiently and precisely disrupt, excise, mutate, insert, or replace genes. However, human embryonic stem cells and induced pluripotent stem cells (iPSCs)...

Authors: SM Byrne, GM Church

SST Peer Reviewed

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system

Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P....

Authors: Mehdi Ghorbal, Molly Gorman, Cameron Ross Macpherson, Rafael Miyazawa Martins, Artur Scherf,...

SST Peer Reviewed

ROS detoxification and proinflammatory cytokines are linked by p38 MAPK signaling in a model of mature astrocyte activation.

Astrocytes are the most abundant glial cell in the retinal nerve fiber layer (NFL) and optic nerve head (ONH), and perform essential roles in maintaining retinal ganglion cell (RGC) detoxification and homeostasis. Mature astrocytes are relatively...

Authors: Nahirnyj A, Livne-Bar I, Guo X, Sivak J.

Efficient generation of integration-free ips cells from human adult peripheral blood using BCL-XL together with Yamanaka factors.

The ability to efficiently generate integration-free induced pluripotent stem cells (iPSCs) from the most readily available source-peripheral blood-has the potential to expedite the advances of iPSC-based therapies. We have successfully generated...

Authors: Su RJ, Baylink DJ, Neises A, Kiroyan JB, Meng X, Payne KJ, Tschudy-Seney B, Duan Y, Appleby N,...

SST Peer Reviewed

A co-culture genome-wide RNAi screen with mammary epithelial cells reveals transmembrane signals required for growth and differentiation

INTRODUCTION: The extracellular signals regulating mammary epithelial cell growth are of relevance to understanding the pathophysiology of mammary epithelia, yet they remain poorly characterized. In this study, we applied an unbiased approach to...

Authors: Burleigh A, McKinney S, Brimhall J, Yap D, Eirew P, Poon S, Ng V, Wan A, Prentice L, Annab L,...

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing.

Monomeric CRISPR-Cas9 nucleases are widely used for targeted genome editing but can induce unwanted off-target mutations with high frequencies. Here we describe dimeric RNA-guided FokI nucleases (RFNs) that can recognize extended sequences and edit...

Authors: Shengdar Q. Tsai, Nicolas Wyvekens, Cyd Khayter, Jennifer A. Foden, Vishal Thapar, Deepak Reyon,...

SST Peer Reviewed

Tuning cytokine receptor signaling by re-orienting dimer geometry with surrogate ligands

Most cell-surface receptors for cytokines and growth factors signal as dimers, but it is unclear whether remodeling receptor dimer topology is a viable strategy to "tune" signaling output. We utilized diabodies (DA) as surrogate ligands in a...

Authors: Moraga I, Wernig G, Wilmes S, Gryshkova V, Richter CP, Hong WJ, Sinha R, Guo F, Fabionar H, Wehrman...

SST Peer Reviewed

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Modulation of the Surface Proteome through Multiple Ubiquitylation Pathways in African Trypanosomes.

Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells

Regulation of the Flt3 Gene in Haematopoietic Stem and Early Progenitor Cells

Current Protocols in Stem Cell Biology: CRISPR-Mediated Gene Targeting of Human Induced Pluripotent Stem Cells.

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system

ROS detoxification and proinflammatory cytokines are linked by p38 MAPK signaling in a model of mature astrocyte activation.

Efficient generation of integration-free ips cells from human adult peripheral blood using BCL-XL together with Yamanaka factors.

A co-culture genome-wide RNAi screen with mammary epithelial cells reveals transmembrane signals required for growth and differentiation

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing.

Tuning cytokine receptor signaling by re-orienting dimer geometry with surrogate ligands