Search Results

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair

Repair of double strand DNA breaks (DSBs) can result in gene disruption or gene modification via homology directed repair (HDR) from donor DNA. Altering cellular responses to DSBs may rebalance editing outcomes towards HDR and away from other repair...

Authors: Wienert B, Nguyen DN, Guenther A, Feng SJ, Locke MN, Wyman SK, Shin J, Kazane KR, Gregory GL, Carter...

Changes in the function of angiotensin II type 1 receptor due to cholesterol depletion from cell membrane

Blockers of G-protein coupled receptors (GPCRs), angiotensin II (Ang II) type 1 (AT1) receptor and ß1-adrenergic (Ad) receptor, have been shown to improve the prognosis of cardiovascular disease. Cholesterol molecules in the cell membrane are needed...

Authors: Matsuo Y, Suematsu Y, Idemoto Y, Kuwano T, Kitajima K, Miura SI

CRISPR-based functional genomics in human dendritic cells

Dendritic cells (DCs) regulate processes ranging from antitumor and antiviral immunity to host-microbe communication at mucosal surfaces. It remains difficult, however, to genetically manipulate human DCs, limiting our ability to probe how DCs elicit...

Authors: Marco Jost, Amy N. Jacobson, Jeffrey A. Hussmann, Giana Cirolia, Michael A. Fischbach, Jonathan S....

Efficient C• G-to-G• C base editors developed using CRISPRi screens, target-library analysis, and machine learning

Programmable C•G-to-G•C base editors (CGBEs) have broad scientific and therapeutic potential, but their editing outcomes have proved difficult to predict and their editing efficiency and product purity are often low. We describe a suite of engineered...

Authors: Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, Newby GA, Yang D, Mok B, Replogle...

Imbalanced host response to SARS-CoV-2 drives development of COVID-19

Viral pandemics, such as the one caused by SARS-CoV-2, pose an imminent threat to humanity. Because of its recent emergence, there is a paucity of information regarding viral behavior and host response following SARS-CoV-2 infection. Here, we offer...

Authors: Daniel Blanco-Melo, Benjamin E. Nilsson-Payant, Wen-Chun Liu, Skyler Uhl, Daisy Hoagland, Rasmus...

CRISPR-Cas9-mediated nuclear transport and genomic integration of nanostructured genes in human primary cells

DNA nanostructures are a promising tool to deliver molecular payloads to cells. DNA origami structures, where long single-stranded DNA is folded into a compact nanostructure, present an attractive approach to package genes; however, effective...

Authors: Lin-Shiao E, Pfeifer WG, Shy BR, Saffari Doost M, Chen E, Vykunta VS, Hamilton JR, Stahl EC, Lopez...

CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells

Prime editors have been delivered using DNA or RNA vectors. Here we demonstrate prime editing with purified ribonucleoprotein complexes. We introduced somatic mutations in zebrafish embryos with frequencies as high as 30% and demonstrate germline...

Authors: Petri K, Zhang W, Ma J, Schmidts A, Lee H, Horng JE, Kim DY, Kurt IC, Clement K, Hsu JY, Pinello L,...

SST Peer Reviewed

Highly efficient genome editing in primary bronchial epithelial cells establishes FOXJ1 as essential for ciliation in human airways

The structure and composition of the bronchial epithelium is altered in respiratory diseases such as COPD and asthma, in which goblet cell hyperplasia and reduced numbers of ciliated cells impair mucociliary clearance. We describe a robust genome...

Authors: Radu Rapiteanu??1, Tina Karagyozova1, Natalie Zimmermann2, Gareth Wayne1, MatteoMartufi1, Nikolai N...

SST Peer Reviewed

Efficient gene knockout in primary human and murine myeloid cells by non-viral delivery of CRISPR-Cas9.

Myeloid cells play critical and diverse roles in mammalian physiology, including tissue development and repair, innate defense against pathogens, and generation of adaptive immunity. As cells that show prolonged recruitment to sites of injury or...

Authors: Freund EC, Lock JY, Oh J, Maculins T, Delamarre L, Bohlen CJ, Haley B, Murthy A.

SST Peer Reviewed

c-Jun overexpression in CAR T cells induces exhaustion resistance

Chimeric antigen receptor (CAR) T cells mediate anti-tumour efects in a small subset of patients with cancer , but dysfunction due to T cell exhaustion is an important barrier to progress. To investigate the biology of exhaustion in human...

Authors: Lynn RC, Weber EW, Sotillo E, Gennert D, Xu P, Good Z, Anbunathan H, Lattin J, Jones R, Tieu V,...

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Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair

Changes in the function of angiotensin II type 1 receptor due to cholesterol depletion from cell membrane

CRISPR-based functional genomics in human dendritic cells

Efficient C• G-to-G• C base editors developed using CRISPRi screens, target-library analysis, and machine learning

Imbalanced host response to SARS-CoV-2 drives development of COVID-19

CRISPR-Cas9-mediated nuclear transport and genomic integration of nanostructured genes in human primary cells

CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells

Highly efficient genome editing in primary bronchial epithelial cells establishes FOXJ1 as essential for ciliation in human airways

Efficient gene knockout in primary human and murine myeloid cells by non-viral delivery of CRISPR-Cas9.

c-Jun overexpression in CAR T cells induces exhaustion resistance