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Local Transplantation of Granulocyte Colony-Stimulating Factor-Mobilized Human Peripheral Blood Mononuclear Cells for Unhealing Bone Fractures

We previously reported the therapeutic potential of human peripheral blood (hPB) CD34(+) cells for bone fracture healing via vasculogenesis/angiogenesis and osteogenesis. Transplantation of not only hPB CD34(+) cells but also hPB total mononuclear...

Authors: Fukui T, Matsumoto T, Mifune Y, Shoji T, Kuroda T, Kawakami Y, Kawamoto A, Ii M, Kawamata S,...

Expression pattern and function of Notch2 in different subtypes of first trimester cytotrophoblast

INTRODUCTION: Notch signalling has been shown to control cytotrophoblast (CTB) proliferation, differentiation and motility suggesting that the conserved signalling pathway could be critical for human placental development. Since individual Notch...

Authors: Plessl K, Haider S, Fiala C, Pollheimer J, Knöfler M

SST Peer Reviewed

Insights into the role of sulfated glycans in cancer cell adhesion and migration through use of branched peptide probe

The tetra-branched peptide NT4 selectively binds to different human cancer cells and tissues. NT4 specifically binds to sulfated glycosaminoglycans on cancer cell membranes. Since sulfated glycosaminoglycans are involved in cancer cell interaction...

Authors: Brunetti J, Depau L, Falciani C, Gentile M, Mandarini E, Riolo G, Lupetti P, Pini A, Bracci L

SST Peer Reviewed

Differential Release of Heterogeneous Human Mesenchymal Stem Cell Populations from Haemarthrotic Traumatic Knee Injury

Surgical reconstruction of the anterior cruciate ligament (ACL) has a protracted healing phase due to poor osseous tissue integration at the graft/host interface. Intervention with an autologous cell-based therapy using human mes-enchymal stem cells...

Authors: David J. Deehan1,2, Daniel J. Dowen2, Andrew P. Sprowson, Linda C. Ferguson, Nilendran S....

Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells.

Targeted nucleases, including zinc-finger nucleases (ZFNs), transcription activator-like (TAL) effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9), have provided...

Authors: Jia Liu, Thomas Gaj, Yifeng Yang, Nan Wang, Sailan Shui, Sojung Kim, Chidananda Nagamangala...

SST Peer Reviewed

Manufacture of clinical-grade CD19-specific T cells stably expressing chimeric antigen receptor using Sleeping Beauty system and artificial antigen presenting cells.

Adoptive transfer of T cells expressing a CD19-specific chimeric antigen receptor (CAR) is being evaluated in multiple clinical trials. Our current approach to adoptive immunotherapy is based on a second generation CAR (designated CD19RCD28) that...

Authors: Singh H, Figliola MJ, Dawson MJ, Olivares S, Zhang L, Yang G, Maiti S, Manuri P, Senyukov V, Jena B,...

SST Peer Reviewed

Inactivation of GDP-fucose transporter gene (Slc35c1) in CHO cells by ZFNs, TALENs and CRISPR-Cas9 for production of fucose-free antibodies.

Removal of core fucose from N-glycans attached to human IgG1 significantly enhances its affinity for the receptor Fc?RIII and thereby dramatically improves its antibody-dependent cellular cytotoxicity (ADCC) activity. While previous works have shown...

Authors: Kah Fai Chan, Wahyu Shahreel, Corrine Wan, Gavin Teo, Noor Hayati, Shi Jie Tay, Wen Han Tong,...

SST Peer Reviewed

High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs.

Targeted endonucleases including zinc finger nucleases (ZFNs) and clustered regularly interspaced short palindromic repeats (CRISPRs)/Cas9 are increasingly being used for genome editing in higher species. We therefore devised a broadly applicable and...

Authors: Duda K, Lonowski LA, Kofoed-Nielsen M, Ibarra A, Delay CM, Kang Q, Yang Z, Pruett-Miller SM, Bennett...

SST Peer Reviewed

Dendritic cell SIRT1–HIF1a axis programs the differentiation of CD4+ T cells through IL-12 and TGF-ß1

The differentiation of naive CD4(+) T cells into distinct lineages plays critical roles in mediating adaptive immunity or maintaining immune tolerance. In addition to being a first line of defense, the innate immune system also actively instructs...

Authors: Liu G1, Bi Y2, Xue L3, Zhang Y4, Yang H4, Chen X4, Lu Y4, Zhang Z4, Liu H4, Wang X4, Wang R5, Chu...

A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells.

Conventional affinity purification followed by mass spectrometry (AP-MS) analysis is a broadly applicable method used to decipher molecular interaction networks and infer protein function. However, it is sensitive to perturbations induced by...

Authors: Mathieu Dalvai, Jeremy Loehr, Karine Jacquet, Caroline C. Huard, Céline Roques, Pauline...

SST Peer Reviewed

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Local Transplantation of Granulocyte Colony-Stimulating Factor-Mobilized Human Peripheral Blood Mononuclear Cells for Unhealing Bone Fractures

Expression pattern and function of Notch2 in different subtypes of first trimester cytotrophoblast

Insights into the role of sulfated glycans in cancer cell adhesion and migration through use of branched peptide probe

Differential Release of Heterogeneous Human Mesenchymal Stem Cell Populations from Haemarthrotic Traumatic Knee Injury

Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells.

Manufacture of clinical-grade CD19-specific T cells stably expressing chimeric antigen receptor using Sleeping Beauty system and artificial antigen presenting cells.

Inactivation of GDP-fucose transporter gene (Slc35c1) in CHO cells by ZFNs, TALENs and CRISPR-Cas9 for production of fucose-free antibodies.

High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs.

Dendritic cell SIRT1–HIF1a axis programs the differentiation of CD4+ T cells through IL-12 and TGF-ß1

A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells.