Search Results

MicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells

While all forms of tobacco exposure have negative health effects, the significance of exposure to electronic cigarettes (eCig) is not fully understood. Here, we studied the global effects of eCig on the micro RNA (miRNA) transcriptome in human lung...

Authors: Solleti SK, Bhattacharya S, Ahmad A, Wang Q, Mereness J, Rangasamy T, Mariani TJ

CRISPR/Cas9-Based Gene Engineering of Human Natural Killer Cells: Protocols for Knockout and Readouts to Evaluate Their Efficacy

Natural killer (NK) cells are cytotoxic lymphocytes of our immune system with the ability to identify and kill certain virally infected and tumor-transformed cells. During the past 15 years, it has become increasingly clear that NK cells are involved...

Authors: Mélanie Lambert , Caroline Leijonhufvud , Filip Segerberg , J Joseph Melenhorst , Mattias Carlsten

Quantitative analysis of the CD4+ T cell response to therapeutic antibodies in healthy donors using a novel T cell:PBMC assay.

Many biopharmaceuticals (BPs) are known to be immunogenic in the clinic, which can result in modified pharmacokinetics, reduced efficacy, allergic reactions and anaphylaxis. During recent years, several technologies to predict immunogenicity have...

Authors: Schultz HS1, Reedtz-Runge SL2, Bäckström BT1, Lamberth K1, Pedersen CR3, Kvarnhammar AM1; ABIRISK...

SST Peer Reviewed

PU.1 Controls Fibroblast Polarization and Tissue Fibrosis

Fibroblasts are polymorphic cells with pleiotropic roles in organ morphogenesis, tissue homeostasis and immune responses. In fibrotic diseases, fibroblasts synthesize abundant amounts of extracellular matrix, which induces scarring and organ failure....

Authors: Thomas Wohlfahrt , Simon Rauber , Steffen Uebe , Markus Luber , Alina Soare , Arif Ekici , Stefanie...

Inhibition of IL-13-induced periostin in airway epithelium attenuates cellular protein expression of MUC5AC.

BACKGROUND AND OBJECTIVE:Serum periostin is increased in asthma and serves as a surrogate marker for IL-13 activity in the lung. Serum levels of periostin are the most robust biomarker predicting a favourable response to the anti-IL-13 drug,...

Authors: Suzaki I, Kawano S, Komiya K, Tanabe T, Akaba T, Asano K, Suzaki H, Izuhara K, Rubin BK

Entry, Intracellular Survival, and Multinucleated-Giant-Cell-Forming Activity of Burkholderia pseudomallei in Human Primary Phagocytic and Nonphagocytic Cells.

The human pathogen Burkholderia pseudomallei and the related species Burkholderia thailandensis are facultative intracellular bacteria characterized by the ability to escape into the cytosol of the host cell and to stimulate the formation of...

Authors: Whiteley L1, Meffert T1, Haug M1, Weidenmaier C1, Hopf V2, Bitschar K3, Schittek B3, Kohler C2,...

microRNA-184 Is Induced by Store-Operated Calcium Entry and Regulates Early Keratinocyte Differentiation

Extracellular calcium (Ca2+ ) and store-operated Ca2+ entry (SOCE) govern homoeostasis in the mammalian epidermis. Multiple microRNAs (miRNA) also regulate epidermal differentiation, and raised external Ca2+ modulates the expression of several such...

Authors: Adam Richardson , Andrew K Powell , Darren W Sexton , Jason L Parsons , Nick J Reynolds , Kehinde...

Inhibition of Replication and Infection of Severe Acute Respiratory Syndrome-Associated Coronavirus With Plasmid-Mediated Interference RNA

Severe acute respiratory syndrome (SARS) is a newly emerged infectious disease caused by a novel coronavirus (SARS-CoV), which spread to over 30 countries in early 2003. Until recently, no specific vaccines and effective drugs have been available to...

Authors: Bing Ni 1 , Xinfu Shi, Yang Li, Wenda Gao, Xiliang Wang, Yuzhang Wu

SST Peer Reviewed

Successfully Transfected Primary Peripherally Mobilized Human CD34+ Hematopoietic Stem and Progenitor Cells (HSPCs) Demonstrate Increased Susceptibility to Retroviral Infection

Transfection, the process of introducing purified nucleic acids into cells, and viral transduction, viral-mediated nucleic acid transfer, are two commonly utilized techniques for gene delivery in the research setting. Transfection allows purified...

Authors: Jeffrey Sebrow , Stephen P Goff, Daniel O Griffin

SST Peer Reviewed

IL-13 regulates IL-17C expression by suppressing NF-?B-mediated transcriptional activation in airway epithelial cells.

The cytokine interleukin (IL)-17C is highly expressed in epithelial tissues and involved in innate immune responses; however, the regulation of IL-17C expression in the airways remains poorly understood. Here, we show that IL-1ß strongly induces both...

Authors: Yamanaka K1, Fujisawa T2, Kusagaya H3, Mori K3, Niwa M3, Furuhashi K4, Kono M4, Hamada E1, Suda T3,...

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MicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells

CRISPR/Cas9-Based Gene Engineering of Human Natural Killer Cells: Protocols for Knockout and Readouts to Evaluate Their Efficacy

Quantitative analysis of the CD4+ T cell response to therapeutic antibodies in healthy donors using a novel T cell:PBMC assay.

PU.1 Controls Fibroblast Polarization and Tissue Fibrosis

Inhibition of IL-13-induced periostin in airway epithelium attenuates cellular protein expression of MUC5AC.

Entry, Intracellular Survival, and Multinucleated-Giant-Cell-Forming Activity of Burkholderia pseudomallei in Human Primary Phagocytic and Nonphagocytic Cells.

microRNA-184 Is Induced by Store-Operated Calcium Entry and Regulates Early Keratinocyte Differentiation

Inhibition of Replication and Infection of Severe Acute Respiratory Syndrome-Associated Coronavirus With Plasmid-Mediated Interference RNA

Successfully Transfected Primary Peripherally Mobilized Human CD34+ Hematopoietic Stem and Progenitor Cells (HSPCs) Demonstrate Increased Susceptibility to Retroviral Infection

IL-13 regulates IL-17C expression by suppressing NF-?B-mediated transcriptional activation in airway epithelial cells.