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Different skeletal effects of the peroxisome proliferator activated receptor (PPAR)alpha agonist fenofibrate and the PPARgamma agonist pioglitazone.

BACKGROUND: All the peroxisome proliferator activated receptors (PPARs) are found to be expressed in bone cells. The PPARgamma agonist rosiglitazone has been shown to decrease bone mass in mice and thiazolidinediones (TZDs) have recently been found...

Authors: Syversen U, Stunes AK, Gustafsson BI, Obrant KJ, Nordsletten L, Berge R, Thommesen L, Reseland JE

SST Peer Reviewed

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

CRISPR-Cas-mediated genome editing relies on guide RNAs that direct site-specific DNA cleavage facilitated by the Cas endonuclease. Here we report that chemical alterations to synthesized single guide RNAs (sgRNAs) enhance genome editing efficiency...

Authors: Ayal Hendel, Rasmus O Bak, Joseph T Clark, Andrew B Kennedy, Daniel E Ryan, Subhadeep Roy, Israel...

SST Peer Reviewed

Potentiation of osteoclastogenesis by adipogenic conversion of bone marrow-derived mesenchymal stem cells.

Bone marrow-derived mesenchymal stem cells (BMSCs) are the indispensable component of the bone marrow, being the common precursors for adipocytes and osteoblasts. We show here that adipogenic differentiation resulted in increase in the production of...

Authors: Mori K, Suzuki K, Hozumi A, Goto H, Tomita M, Koseki H, Yamashita S, Osaki M

SST Peer Reviewed

Reprogramming LCLs to iPSCs Results in Recovery of Donor-Specific Gene Expression Signature.

Renewable in vitro cell cultures, such as lymphoblastoid cell lines (LCLs), have facilitated studies that contributed to our understanding of genetic influence on human traits. However, the degree to which cell lines faithfully maintain differences...

Authors: Thomas SM, Kagan C, Pavlovic BJ, Burnett J, Patterson K, Pritchard JK, Gilad Y

SST Peer Reviewed

Sleeping Beauty Transposition of Chimeric Antigen Receptors Targeting Receptor Tyrosine Kinase-Like Orphan Receptor-1 (ROR1) into Diverse Memory T-Cell Populations.

T cells modified with chimeric antigen receptors (CARs) targeting CD19 demonstrated clinical activity against some B-cell malignancies. However, this is often accompanied by a loss of normal CD19+ B cells and humoral immunity. Receptor tyrosine...

Authors: Deniger DC, Yu J, Huls MH, Figliola MJ, Mi T, Maiti SN, Widhopf GF 2nd, Hurton LV, Thokala R, Singh...

SST Peer Reviewed

JAK inhibition with tofacitinib suppresses arthritic joint structural damage through decreased RANKL production

OBJECTIVE: The mechanistic link between Janus kinase (JAK) signaling and structural damage to arthritic joints in rheumatoid arthritis (RA) is poorly understood. This study was undertaken to investigate how selective inhibition of JAK with...

Authors: LaBranche TP, Jesson MI, Radi ZA, Storer CE, Guzova JA, Bonar SL, Thompson JM, Happa FA, Stewart ZS,...

SST Peer Reviewed

Regulation of normal and cystic fibrosis airway epithelial repair processes by TNF-a after injury.

Chronic infection and inflammation have been associated with progressive airway epithelial damage in patients with cystic fibrosis (CF). However, the effect of inflammatory products on the repair capacity of respiratory epithelia is unclear. Our...

Authors: Maillé E, Trinh NT, Privé A, Bilodeau C, Bissonnette E, Grandvaux N, Brochiero E.

Metal-sulfate induced generation of ROS in human brain cells: detection using an isomeric mixture of 5- and 6-carboxy-2\\\',7\\\'-dichlorofluorescein diacetate (carboxy-DCFDA) as a cell permeant tracer.

Evolution of reactive oxygen species (ROS), generated during the patho-physiological stress of nervous tissue, has been implicated in the etiology of several progressive human neurological disorders including Alzheimer's disease (AD) and amylotrophic...

Authors: Pogue AI, Jones BM, Bhattacharjee S, Percy ME, Zhao Y, Lukiw WJ

SST Peer Reviewed

Production and Assessment of Decellularized Pig and Human Lung Scaffolds

The authors have previously shown that acellular (AC) trachea-lung scaffolds can (1) be produced from natural rat lungs, (2) retain critical components of the extracellular matrix (ECM) such as collagen-1 and elastin, and (3) be used to produce lung...

Authors: Joan E. Nichols, PhD, Jean Niles, MA, Michael Riddle, MD, Gracie Vargas, PhD, Tuya Schilagard,...

Piperine Congeners as Inhibitors of Vascular Smooth Muscle Cell Proliferation.

Successful vascular healing after percutaneous coronary interventions is related to the inhibition of abnormal vascular smooth muscle cell proliferation and efficient re-endothelialization. In the search for vascular smooth muscle cell...

Authors: Mair CE, Liu R, Atanasov AG, Wimmer L, Nemetz-Fiedler D, Sider N, Heiss EH1, Mihovilovic MD, Dirsch...

SST Peer Reviewed

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Different skeletal effects of the peroxisome proliferator activated receptor (PPAR)alpha agonist fenofibrate and the PPARgamma agonist pioglitazone.

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

Potentiation of osteoclastogenesis by adipogenic conversion of bone marrow-derived mesenchymal stem cells.

Reprogramming LCLs to iPSCs Results in Recovery of Donor-Specific Gene Expression Signature.

Sleeping Beauty Transposition of Chimeric Antigen Receptors Targeting Receptor Tyrosine Kinase-Like Orphan Receptor-1 (ROR1) into Diverse Memory T-Cell Populations.

JAK inhibition with tofacitinib suppresses arthritic joint structural damage through decreased RANKL production

Regulation of normal and cystic fibrosis airway epithelial repair processes by TNF-a after injury.

Metal-sulfate induced generation of ROS in human brain cells: detection using an isomeric mixture of 5- and 6-carboxy-2\\\',7\\\'-dichlorofluorescein diacetate (carboxy-DCFDA) as a cell permeant tracer.

Production and Assessment of Decellularized Pig and Human Lung Scaffolds

Piperine Congeners as Inhibitors of Vascular Smooth Muscle Cell Proliferation.