Search Results

Defining suitable reference genes for RT-qPCR analysis on human sertoli cells after 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure.

Quantitative real-time RT-PCR (qPCR) has proven to be a valuable molecular technique to quantify gene expression. There are few studies in the literature that describe suitable reference genes to normalize gene expression data. Studies of...

Authors: Ribeiro MA, dos Reis MB, de Moraes LN, Briton-Jones C, Rainho CA, Scarano WR

SST Peer Reviewed

Engineered composite tissue as a bioartificial limb graft.

The loss of an extremity is a disastrous injury with tremendous impact on a patient's life. Current mechanical prostheses are technically highly sophisticated, but only partially replace physiologic function and aesthetic appearance. As a biologic...

Authors: Jank BJ, Xiong L, Moser PT, Guyette JP, Ren X, Cetrulo CL, Leonard DA, Fernandez L, Fagan SP, Ott...

SST Peer Reviewed

Diploid chromosome number and chromosomal variation in the white rhinoceros (Ceratotherium simum).

Chromosomal studies were conducted on 38 white rhinoceroses representing both the northern and southern subspecies and one subspecies hybrid. Improvements in tissue culture methods and harvesting techniques have made it possible to obtain a highly...

Authors: Houck ML, Ryder OA, Váhala J, Kock RA, Oosterhuis JE

Interactions between aCaMKII and calmodulin in living cells: conformational changes arising from CaM-dependent and -independent relationships

BACKGROUND: aCaMKII plays central and essential roles in long-term potentiation (LTP), learning and memory. aCaMKII is activated via binding with Ca²?/CaM in response to elevated Ca²? concentration. Furthermore, prolonged increase in Ca²?...

Authors: Kato K, Iwamoto T, Kida S.

Assessment of an Efficient Xeno-Free Culture System of Human Periodontal Ligament Stem Cells

The possibility of transplanting adult stem cells into damaged organs has opened new prospects for the treatment of several human pathologies. The purpose of this study was to develop a culture system for the expansion and production of human...

Authors: Trubiani O, Piattelli A, Gatta V, Marchisio M, Diomede F, D\\\'Aurora M, Merciaro I, Pierdomenico L,...

SST Peer Reviewed

Characterization of age-related changes of tendon stem cells from adult human tendons.

PURPOSE: The present study evaluated the presence of stem cells in hamstring tendons from adult subjects of different ages. The aim was to isolate, characterize and expand these cells in vitro, and to evaluate whether cell activities are influenced...

Authors: Ruzzini L, Abbruzzese F, Rainer A, Longo UG, Trombetta M, Maffulli N, Denaro V

SST Peer Reviewed

The excimer lamp induces cutaneous nerve degeneration and reduces scratching in a dry-skin mouse model.

Epidermal hyperinnervation, which is thought to underlie intractable pruritus, has been observed in patients with atopic dermatitis (AD). The epidermal expression of axonal guidance molecules has been reported to regulate epidermal hyperinnervation....

Authors: Kamo A, Tominaga M, Kamata Y, Kaneda K, Ko K2, Matsuda H, Kimura U, Ogawa H, Takamori K.

Fast and Efficient Neural Conversion of Human Hematopoietic Cells

Neurons obtained directly from human somatic cells hold great promise for disease modeling and drug screening. Available protocols rely on overexpression of transcription factors using integrative vectors and are often slow, complex, and inefficient....

Authors: Castaño J, Menendez P, Bruzos-Cidon C, Straccia M, Sousa A5, Zabaleta L, Vazquez N, Zubiarrain A,...

SST Peer Reviewed

Characterization of the CD14++CD16+ monocyte population in human bone marrow.

Numerous studies have divided blood monocytes according to their expression of the surface markers CD14 and CD16 into following subsets: classical CD14(++)CD16(-), intermediate CD14(++)CD16(+) and nonclassical CD14(+)CD16(++) monocytes. These subsets...

Authors: Mandl M, Schmitz S, Weber C, Hristov M

SST Peer Reviewed

Competitive Fitness in Coronaviruses Is Not Correlated with Size or Number of Double-Membrane Vesicles under Reduced-Temperature Growth Conditions

Positive-stranded viruses synthesize their RNA in membrane-bound organelles, but it is not clear how this benefits the virus or the host. For coronaviruses, these organelles take the form of double-membrane vesicles (DMVs) interconnected by a...

Authors: Hawaa M. N. Al-Mulla, Lauren Turrell, Nicola M. Smith, Luke Payne, Surendranath Baliji, Roland...

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Defining suitable reference genes for RT-qPCR analysis on human sertoli cells after 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure.

Engineered composite tissue as a bioartificial limb graft.

Diploid chromosome number and chromosomal variation in the white rhinoceros (Ceratotherium simum).

Interactions between aCaMKII and calmodulin in living cells: conformational changes arising from CaM-dependent and -independent relationships

Assessment of an Efficient Xeno-Free Culture System of Human Periodontal Ligament Stem Cells

Characterization of age-related changes of tendon stem cells from adult human tendons.

The excimer lamp induces cutaneous nerve degeneration and reduces scratching in a dry-skin mouse model.

Fast and Efficient Neural Conversion of Human Hematopoietic Cells

Characterization of the CD14++CD16+ monocyte population in human bone marrow.

Competitive Fitness in Coronaviruses Is Not Correlated with Size or Number of Double-Membrane Vesicles under Reduced-Temperature Growth Conditions