Search Results

Adenosine regulates bone metabolism via A1, A2A, and A2B receptors in bone marrow cells from normal humans and patients with multiple myeloma.

Multiple myeloma (MM) is characterized by osteolytic bone lesions with uncoupled bone remodeling. In this study, we examined the effects of adenosine and its receptors (A1R, A2AR, A2BR, and A3R) on osteoblast and osteoclast differentiation of cells...

Authors: He W, Mazumder A, Wilder T, Cronstein BN

SST Peer Reviewed

Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9

Genome editing via CRISPR/Cas9 has rapidly become the tool of choice by virtue of its efficacy and ease of use. However, CRISPR/Cas9-mediated genome editing in clinically relevant human somatic cells remains untested. Here, we report CRISPR/Cas9...

Authors: Mandal PK, Ferreira LM, Collins R, Meissner TB, Boutwell CL, Friesen M, Vrbanac V, Garrison BS,...

SST Peer Reviewed

Initial observations on the effect of medium composition on the differentiation of murine embryonic stem cells to alveolar type II cells.

The pluripotency and high proliferative index of embryonic stem (ES) cells make them a good potential source of cells for tissue engineering purposes. We have shown that ES cells can be induced to differentiate in vitro into pulmonary epithelial...

Authors: Rippon HJ, Ali NN, Polak JM, Bishop AE.

SOX4 mediates TGF-ß-induced expression of mesenchymal markers during mammary cell epithelial to mesenchymal transition.

The epithelial to mensenchymal transition program regulates various aspects of embryonic development and tissue homeostasis, but aberrant activation of this pathway in cancer contributes to tumor progression and metastasis. TGF-b potently induces an...

Authors: Vervoort SJ, Lourenço AR, van Boxtel R, Coffer PJ.

Targeted genome modification in mice using zinc-finger nucleases. Genetics

Homologous recombination-based gene targeting using Mus musculus embryonic stem cells has greatly impacted biomedical research. This study presents a powerful new technology for more efficient and less time-consuming gene targeting in mice using...

Authors: Carbery ID, Ji D, Harrington A, Brown V, Weinstein EJ, Liaw L, Cui X

Derivation of lung epithelium from human cord blood-derived mesenchymal stem cells

RATIONALE: Recent studies have suggested that both embryonic stem cells and adult bone marrow stem cells can participate in the regeneration and repair of diseased adult organs, including the lungs. However, the extent of airway epithelial...

Authors: Sueblinvong V, Loi R, Eisenhauer PL, Bernstein IM, Suratt BT, Spees JL, Weiss DJ.

Metabolism of melatonin and biological activity of intermediates of melatoninergic pathway in human skin cells

Indolic and kynuric pathways of skin melatonin metabolism were monitored by liquid chromatography mass spectrometry in human keratinocytes, melanocytes, dermal fibroblasts, and melanoma cells. Production of 6-hydroxymelatonin [6(OH)M],...

Authors: Kim TK1, Kleszczynski K, Janjetovic Z, Sweatman T, Lin Z, Li W, Reiter RJ, Fischer TW, Slominski...

Stimulation of Activin A/Nodal signaling is insufficient to induce definitive endoderm formation of cord blood-derived unrestricted somatic stem cells.

NTRODUCTION: Unrestricted somatic stem cells (USSC) derived from umbilical cord blood are an attractive alternative to human embryonic stem cells (hESC) for cellular therapy. USSC are capable of forming cells representative of all three germ line...

Authors: Filby CE, Williamson R, van Kooy P, Pébay A, Dottori M, Elwood NJ, Zaibak F.

Characterization of dystrophin deficient rats: a new model for Duchenne muscular dystrophy.

A few animal models of Duchenne muscular dystrophy (DMD) are available, large ones such as pigs or dogs being expensive and difficult to handle. Mdx (X-linked muscular dystrophy) mice only partially mimic the human disease, with limited chronic...

Authors: Larcher T, Lafoux A, Tesson L, Remy S, Thepenier V, François V, Le Guiner C, Goubin H, Dutilleul M,...

SST Peer Reviewed

Regulatory and functional connection of microphthalmia-associated transcription factor and anti-metastatic pigment epithelium derived factor in melanoma

Pigment epithelium-derived factor (PEDF), a member of the serine protease inhibitor superfamily, has potent anti-metastatic effects in cutaneous melanoma through its direct actions on endothelial and melanoma cells. Here we show that PEDF expression...

Authors: Fernández-Barral A, Orgaz JL, Baquero P, Ali Z, Moreno A, Tiana M, Gómez V, Riveiro-Falkenbach E,...

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Adenosine regulates bone metabolism via A1, A2A, and A2B receptors in bone marrow cells from normal humans and patients with multiple myeloma.

Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9

Initial observations on the effect of medium composition on the differentiation of murine embryonic stem cells to alveolar type II cells.

SOX4 mediates TGF-ß-induced expression of mesenchymal markers during mammary cell epithelial to mesenchymal transition.

Targeted genome modification in mice using zinc-finger nucleases. Genetics

Derivation of lung epithelium from human cord blood-derived mesenchymal stem cells

Metabolism of melatonin and biological activity of intermediates of melatoninergic pathway in human skin cells

Stimulation of Activin A/Nodal signaling is insufficient to induce definitive endoderm formation of cord blood-derived unrestricted somatic stem cells.

Characterization of dystrophin deficient rats: a new model for Duchenne muscular dystrophy.

Regulatory and functional connection of microphthalmia-associated transcription factor and anti-metastatic pigment epithelium derived factor in melanoma