Search Results

Chemical Conversion of Human Fibroblasts into Functional Schwann Cells

Direct transdifferentiation of somatic cells is a promising approach to obtain patient-specific cells for numerous applications. However, conversion across germ-layer borders often requires ectopic gene expression with unpredictable side effects....

Authors: Thoma EC, Merkl C, Heckel T, Haab R, Knoflach F, Nowaczyk C, Flint N, Jagasia R, Zoffmann SJ, Truong...

SST Peer Reviewed

Validation of Endothelin B Receptor Antibodies Reveals Two Distinct Receptor-related Bands on Western Blot.

Antibodies are important tools for the study of protein expression, but are often used without full validation. In this study, we use Western blots to characterize antibodies targeted to the N- (NT) or C-termini (CT) and the second (IL2) or third...

Authors: Barr TP, Kornberg D, Montmayeur JP, Long M, Reichheld S, Strichartz GR.

SST Peer Reviewed

Transduction of Human Primitive Repopulating Hematopoietic Cells With Lentiviral Vectors Pseudotyped With Various Envelope Proteins

Lentiviral vectors are useful for transducing primitive hematopoietic cells. We examined four envelope proteins for their ability to mediate lentiviral transduction of mobilized human CD34+ peripheral blood cells. Lentiviral particles encoding green...

Authors: Kim YS, Wielgosz MM, Hargrove P, Kepes S, Gray J, Persons DA, Nienhuis AW

SST Peer Reviewed

DrCell – A Software Tool for the Analysis of Cell Signals Recorded with Extracellular Microelectrodes

Microelectrode arrays (MEAs) have been applied for in vivo and in vitro recording and stimulation of electrogenic cells, namely neurons and cardiac myocytes, for almost four decades. Extracellular recordings using the MEA technique inflict minimum...

Authors: Nick C, Goldhammer M, Bestel R, Steger F, Daus AW, Thielemann C

SST Peer Reviewed

ELF4 is critical for induction of type I interferon and the host antiviral response.

Induction of type I interferon is a central event of innate immunity, essential for host defense. Here we report that the transcription factor ELF4 is induced by type I interferon and upregulates interferon expression in a feed-forward loop. ELF4...

Authors: You F, Wang P, Yang L, Yang G, Zhao YO, Qian F, Walker W, Sutton R, Montgomery R, Lin R, Iwasaki A,...

SST Peer Reviewed

The effect of lung cancer on cytokine expression in peripheral blood mononuclear cells.

The purpose of this study is to evaluate cytokine expression by peripheral blood mononuclear cells (PBMC) from stage I lung cancer patients and to confirm these expression patterns by exposing PBMCs to lung cancer cells in vitro. Five altered...

Authors: Chang DH, Rutledge JR, Patel AA, Heerdt BG, Augenlicht LH, Korst RJ.

IL-2/B7.1 (CD80) fusagene transduction of AML blasts by a self-inactivating lentiviral vector stimulates T cell responses in vitro: a strategy to generate whole cell vaccines for AML.

Combined expression of costimulatory factors and proinflammatory cytokines stimulate effective immune-mediated tumor rejection in a variety of murine tumor models. Specifically, syngeneic tumor cells genetically modified to express B7.1 (CD80) have...

Authors: Chan L, Hardwick N, Darling D, Galea-Lauri J, Gäken J, Devereux S, Kemeny M, Mufti G, Farzaneh F.

SST Peer Reviewed

Activity of PLCe contributes to chemotaxis of fibroblasts towards PDGF.

Cell chemotaxis, such as migration of fibroblasts towards growth factors during development and wound healing, requires precise spatial coordination of signalling events. Phosphoinositides and signalling enzymes involved in their generation and...

Authors: Martins M, Warren S, Kimberley C, Margineanu A, Peschard P, McCarthy A, Yeo M, Marshall CJ, Dunsby...

SST Peer Reviewed

Inducible tumor necrosis factor (TNF) receptor-associated factor-1 expression couples the canonical to the non-canonical NF-?B pathway in TNF stimulation

The NF-?B transcription factor mediates the inflammatory response through distinct (canonical and non-canonical) signaling pathways. The mechanisms controlling utilization of either of these pathways are largely unknown. Here we observe that TNF...

Authors: Choudhary S, Kalita M, Fang L, Patel KV, Tian B, Zhao Y, Edeh CB, Brasier AR

Inhibition of protein phosphatase 2A activity by PI3K? regulates ß-adrenergic receptor function.

Phosphoinositide 3-kinase ? (PI3K?) is activated by G protein-coupled receptors (GPCRs). We show here that PI3K? inhibits protein phosphatase 2A (PP2A) at the ß-adrenergic receptor (ßAR, a GPCR) complex altering G protein coupling. PI3K? inhibition...

Authors: Vasudevan NT, Mohan ML, Gupta MK, Hussain AK, Naga Prasad SV.

SST Peer Reviewed

Data Type

Category

Research Area

Platform

Topic

Species

Characteristic

Format

Origin

Chemical Conversion of Human Fibroblasts into Functional Schwann Cells

Validation of Endothelin B Receptor Antibodies Reveals Two Distinct Receptor-related Bands on Western Blot.

Transduction of Human Primitive Repopulating Hematopoietic Cells With Lentiviral Vectors Pseudotyped With Various Envelope Proteins

DrCell – A Software Tool for the Analysis of Cell Signals Recorded with Extracellular Microelectrodes

ELF4 is critical for induction of type I interferon and the host antiviral response.

The effect of lung cancer on cytokine expression in peripheral blood mononuclear cells.

IL-2/B7.1 (CD80) fusagene transduction of AML blasts by a self-inactivating lentiviral vector stimulates T cell responses in vitro: a strategy to generate whole cell vaccines for AML.

Activity of PLCe contributes to chemotaxis of fibroblasts towards PDGF.

Inducible tumor necrosis factor (TNF) receptor-associated factor-1 expression couples the canonical to the non-canonical NF-?B pathway in TNF stimulation

Inhibition of protein phosphatase 2A activity by PI3K? regulates ß-adrenergic receptor function.