Search Results

MARCKS and related chaperones bind to unconventional myosin V isoforms in airway epithelial cells

We have shown previously that myristoylated alanine-rich C kinase substrate (MARCKS) is a key regulatory molecule in the process of mucin secretion by airway epithelial cells, and that part of the secretory mechanism involves intracellular...

Authors: Lin KW, Fang S, Park J, Crews AL, Adler KB.

Efficient non-viral transfection of THP-1 cells

Macrophages are an important part of the cellular immune system and play a key role during immune responses. Thus, macrophages are interesting targets in basic and clinical research. Primary monocytes or monocyte-derived macrophages do not...

Authors: Schnoor M, Buers I, Sietmann A, Brodde MF, Hofnagel O, Robenek H, Lorkowski S.

SST Peer Reviewed

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant.

Viral infections cause morbidity and mortality in allogeneic hematopoietic stem cell transplant (HSCT) recipients. We and others have successfully generated and infused T-cells specific for Epstein Barr virus (EBV), cytomegalovirus (CMV) and...

Authors: Gerdemann U, Vera JF, Rooney CM, Leen AM.

SST Peer Reviewed

Characterization of extended co-culture of non-typeable Haemophilus influenzae with primary human respiratory tissues

Non-typeable Haemophilus influenzae (NTHi) are human-adapted Gram-negative bacteria that comprise part of the normal flora of the human upper airway, but are also responsible for a number of mucosal infections such as otitis media and bronchitis....

Authors: Ren D, Nelson KL, Uchakin PN, Smith AL, Gu XX, Daines DA.

Nucleofection of whole murine retinas.

The mouse retina constitutes an important research model for studies aiming to unravel the cellular and molecular mechanisms underlying ocular diseases. The accessibility of this tissue and its feasibility to directly obtain neurons from it has...

Authors: Gomez-Touriño IM, Senra A, Garcia F.

SST Peer Reviewed

Reassortment between Avian H5N1 and human influenza viruses is mainly restricted to the matrix and neuraminidase gene segments

Highly pathogenic avian influenza H5N1 viruses have devastated the poultry industry in many countries of the eastern hemisphere. Occasionally H5N1 viruses cross the species barrier and infect humans, sometimes with a severe clinical outcome. When...

Authors: Schrauwen EJ, Bestebroer TM, Rimmelzwaan GF, Osterhaus AD, Fouchier RA, Herfst S.

Concise review: putting a finger on stem cell biology: zinc finger nuclease-driven targeted genetic editing in human pluripotent stem cells.

Human pluripotent stem cells (hPSCs) encompassing human embryonic stem cells and human induced pluripotent stem cells (hiPSCs) have a wide appeal for numerous basic biology studies and for therapeutic applications because of their potential to give...

Authors: Collin J, Lako M.

SST Peer Reviewed

Evaluation of differentiated human bronchial epithelial cell culture systems for asthma research.

The aim of the current study was to evaluate primary (human bronchial epithelial cells, HBEC) and non-primary (Calu-3, BEAS-2B, BEAS-2B R1) bronchial epithelial cell culture systems as air-liquid interface- (ALI-) differentiated models for asthma...

Authors: Stewart CE, Torr EE, Mohd Jamili NH, Bosquillon C, Sayers I.

Improved protocol for efficient nonviral transfection of premature THP-1 macrophages.

INTRODUCTION: The human monocytic leukemia cell line THP-1 is a widely used model for investigating monocyte and macrophage biology. Successful transfection of THP-1 monocytes with subsequent phorbol 12-myristate 13-acetate (PMA)-induced...

Authors: Maess MB, Buers I, Robenek H, Lorkowski S.

SST Peer Reviewed

Cadmium increases HIF-1 and VEGF expression through ROS, ERK, and AKT signaling pathways and induces malignant transformation of human bronchial epithelial cells.

Cadmium is categorized as a human carcinogen especially involved in lung cancers. Angiogenesis is considered a fundamental requirement for tumorigenesis, but the mechanisms underlying the tumor angiogenesis induced by cadmium are poorly understood....

Authors: Jing Y, Liu LZ, Jiang Y, Zhu Y, Guo NL, Barnett J, Rojanasakul Y, Agani F, Jiang BH.

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Topic

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MARCKS and related chaperones bind to unconventional myosin V isoforms in airway epithelial cells

Efficient non-viral transfection of THP-1 cells

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant.

Characterization of extended co-culture of non-typeable Haemophilus influenzae with primary human respiratory tissues

Nucleofection of whole murine retinas.

Reassortment between Avian H5N1 and human influenza viruses is mainly restricted to the matrix and neuraminidase gene segments

Concise review: putting a finger on stem cell biology: zinc finger nuclease-driven targeted genetic editing in human pluripotent stem cells.

Evaluation of differentiated human bronchial epithelial cell culture systems for asthma research.

Improved protocol for efficient nonviral transfection of premature THP-1 macrophages.

Cadmium increases HIF-1 and VEGF expression through ROS, ERK, and AKT signaling pathways and induces malignant transformation of human bronchial epithelial cells.