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Efficient oligonucleotide-mediated degradation of nuclear noncoding RNAs in mammalian cultured cells

Recent large-scale transcriptome analyses have revealed that large numbers of noncoding RNAs (ncRNAs) are transcribed from mammalian genomes. They include small nuclear RNAs (snRNAs), small nucleolar RNAs (snoRNAs), and longer ncRNAs, many of which...

Authors: Ideue T, Hino K, Kitao S, Yokoi T, Hirose T

SST Peer Reviewed

Negative regulators of insulin signaling revealed in a genome-wide functional screen

BACKGROUND: Type 2 diabetes develops due to a combination of insulin resistance and beta-cell failure and current therapeutics aim at both of these underlying causes. Several negative regulators of insulin signaling are known and are the subject of...

Authors: Huang SM, Hancock MK, Pitman JL, Orth AP, Gekakis N

Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi.

BACKGROUND: Trypanosoma cruzi, a kinetoplastid protozoan parasite that causes Chagas disease, infects approximately 15 million people in Central and South America. In contrast to the substantial in silico studies of the T. cruzi genome,...

Authors: Xu D, BrandÜ¡n CP, BasombrÜo MA, Tarleton RL.

SST Peer Reviewed

Enhancer of Zeste Homolog 2 Induces Pulmonary Artery Smooth Muscle Cell Proliferation

Introduction Pulmonary Arterial Hypertension (PAH) is a progressively devastating disease characterized by excessive proliferation of the Pulmonary Arterial Smooth Muscle Cells (PASMCs). Studies suggest that PAH and cancers share an...

Authors: Salman A. Aljubran, Ruan Cox, Jr, Prasanna Tamarapu Parthasarathy, Gurukumar Kollongod Ramanathan,...

SST Peer Reviewed

A novel phosphatase cascade regulates differentiation in Trypanosoma brucei via a glycosomal signaling pathway.

In the mammalian bloodstream, the sleeping sickness parasite Trypanosoma brucei is held poised for transmission by the activity of a tyrosine phosphatase, TbPTP1. This prevents differentiation of the transmissible "stumpy forms" until entry into the...

Authors: Szöor B, Ruberto I, Burchmore R, Matthews KR.

Selective regulation of heme oxygenase-1 expression and function by insulin through IRS1/PI3-kinase/AKT-2 pathway

Heme oxygenase 1 (HO-1) is a representative mediator of antioxidants and cytoprotectants against various stress stimuli including oxidants in vascular cells. Intensive insulin treatment can delay the onset and progression of diabetic retinopathy and...

Authors: Geraldes P, Yagi K, Ohshiro Y, He Z, Maeno Y, Yamamoto-Hiraoka J, Rask-Madsen C, Chung SW, Perrella...

Site-specific DNA double-strand breaks greatly increase stable transformation efficiency in Trypanosoma brucei.

Genetic manipulation in African trypanosomes typically relies upon electroporation with chromosomal integration of DNA constructs by homologous recombination. Relatively little is known about chromosomal recombination and repair in these organisms...

Authors: Glover L, Horn D.

SST Peer Reviewed

Comparative transfection of DNA into primary and transformed mammalian cells from different lineages

BACKGROUND: The delivery of DNA into human cells has been the basis of advances in the understanding of gene function and the development of genetic therapies. Numerous chemical and physical approaches have been used to deliver the DNA, but their...

Authors: Maurisse R, De Semir D, Emamekhoo H, Bedayat B, Abdolmohammadi A, Parsi H, Gruenert DC

SST Peer Reviewed

Attenuation of HIV-associated human B cell exhaustion by siRNA downregulation of inhibitory receptors.

Chronic immune activation in HIV-infected individuals leads to accumulation of exhausted tissue-like memory B cells. Exhausted lymphocytes display increased expression of multiple inhibitory receptors, which may contribute to the inefficiency of...

Authors: Kardava L, Moir S, Wang W, Ho J, Buckner CM, Posada JG, O'Shea MA, Roby G, Chen J, Sohn HW, Chun TW,...

SST Peer Reviewed

Transcriptome comparison of human neurons generated using induced pluripotent stem cells derived from dental pulp and skin fibroblasts.

Induced pluripotent stem cell (iPSC) technology is providing an opportunity to study neuropsychiatric disorders through the capacity to grow patient-specific neurons in vitro. Skin fibroblasts obtained by biopsy have been the most reliable source of...

Authors: Chen J, Lin M, Foxe JJ, Pedrosa E, Hrabovsky A, Carroll R, Zheng D, Lachman HM.

SST Peer Reviewed

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Efficient oligonucleotide-mediated degradation of nuclear noncoding RNAs in mammalian cultured cells

Negative regulators of insulin signaling revealed in a genome-wide functional screen

Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi.

Enhancer of Zeste Homolog 2 Induces Pulmonary Artery Smooth Muscle Cell Proliferation

A novel phosphatase cascade regulates differentiation in Trypanosoma brucei via a glycosomal signaling pathway.

Selective regulation of heme oxygenase-1 expression and function by insulin through IRS1/PI3-kinase/AKT-2 pathway

Site-specific DNA double-strand breaks greatly increase stable transformation efficiency in Trypanosoma brucei.

Comparative transfection of DNA into primary and transformed mammalian cells from different lineages

Attenuation of HIV-associated human B cell exhaustion by siRNA downregulation of inhibitory receptors.

Transcriptome comparison of human neurons generated using induced pluripotent stem cells derived from dental pulp and skin fibroblasts.