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Can I download the Software Install Instructions for the FlashGel® Camera from your website?  

Please check the serial number of your camera.For cameras shipped after June 2025 (serial # >3814), Install Instructions and User’s Guide for Software version 3.5.0.0 is available for download...

PyroCell® MAT System: How do I store the cell culture medium before use in an MAT assay?

We recommend to store the unopened bottle of cell culture medium (IMDM) as stipulated by the manufacturer. An opened bottle shall be stored cooled (2-8°C).Before use with MAT assays, the cell culture medium should be equilibrated to room...

How many product samples can I test with one PyroCell® MAT System Kit?

The PyroCell™ MAT System kits contains 3 vials of MAT cells (pMAT cells) and 3 vials of a dedicated MAT Culture Medium Supplement, as well as PeliKine compact ELISA assay components sufficient to run 3x 96-well plates.One vial of pMAT cells...

PyroCell® MAT System Kit: Can I use a different cell culture supplement during the stimulation of pMAT cells?

Alternative supplements may impact the reactivity of the pMAT cells towards endotoxin and Non-endotoxin Pyrogens (NEPs). If an alternative supplement is used, a new qualification of the MAT test is required. The optimized MAT cell culture...


When performing the PyroCell® Monocyte Activation Test, can I use a different cell culture medium than the IMDM (Iscoves modified Dulbeccos medium) recommended by Lonza?

Yes. However, the recommended medium has been validated with the PeliKine Human IL-6 ELISA Rapid Kit included in the PyroCell® MAT System. Please note that experience with alternative cell culture medium is limited. The IMDM 12-722F from Lonza...

Why do I need endotoxin-free materials to conduct the PyroCell® Monocyte Activation Test (MAT)?

For monocyte stimulation it is mandatory to use materials that do not contain assayable levels of endotoxin to ensure that interactions with the TLRs and subsequent release of e.g. IL-6 is due to a contaminant in the test sample only. The assay...

When using the PyroCell® MAT System Kit: Can I store remaining culture supernatant from the MAT stimulation?

Yes, for the IL-6 cytokine measurement. Remaining cell culture supernatant may be frozen at -20°C or -80°C for future use, e.g. retesting. Storage time of the frozen supernatant may be also dependent on the test substance used for stimulation of...

Does the reported Log4j vulnerability threat affect our WinKQCL software (Catalog # 25-611E)?

Thank you for using Lonza Endotoxin Detection Products.Lonza has been providing endotoxin detection products and services since the 1970s, leading the way in innovation of products, methods and software.Our dedicated WinKQCL software is on the market...

The Nucleofector Kits are shipped at room temperature, does this affect the performance?

We certify that the quality of the Nucleofector™ Solution and Kits distributed by Lonza Cologne GmbH is not impaired by shipping the goods at room temperature. Quality is guaranteed at room temperature 4 weeks at least. We recommend keeping...
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