Search Results

The Anti-Inflammatory Effects of Adiponectin Are Mediated via a Heme Oxygenase-1–Dependent Pathway in Rat Kupffer Cells

Altered expression and activity of immunomodulatory cytokines plays a major role in the pathogenesis of alcoholic liver disease. Chronic ethanol feeding increases the sensitivity of Kupffer cells, the resident hepatic macrophage, to...

Authors: Palash Mandal, Pil-Hoon Park, Megan R. McMullen, Brian T. Pratt, and Laura E. Nagy

The stargazin-related protein gamma 7 interacts with the mRNA-binding protein heterogeneous nuclear ribonucleoprotein A2 and regulates the stability of specific mRNAs, including CaV2.2

The role(s) of the novel stargazin-like gamma-subunit proteins remain controversial. We have shown previously that the neuron-specific gamma7 suppresses the expression of certain calcium channels, particularly Ca(V)2.2, and is therefore unlikely to...

Authors: Ferron L, Davies A, Page KM, Cox DJ, Leroy J, Waithe D, Butcher AJ, Sellaturay P, Bolsover S, Pratt...

Glucocorticoids sensitize the innate immune system through regulation of the NLRP3 inflammasome.

Glucocorticoids have long been recognized as powerful anti-inflammatory compounds that are one of the most widely prescribed classes of drugs in the world. However, their role in the regulation of innate immunity is not well understood. We sought to...

Authors: Busillo JM, Azzam KM, Cidlowski JA.

Severe impairment of IFN-? and IFN-? responses in cells of a patient with a novel STAT1 splicing mutation.

Subjects affected by Signal Transducer and Activator of Transcription 1 (STAT1) deficiency have lethal bacterial and viral infections. Complete STAT1 deficiency is inherited as an autosomal recessive disease; partial STAT1 deficiency is inherited as...

Authors: Vairo D, Tassone L, Tabellini G, Tamassia N, Gasperini S, Bazzoni F, Plebani A, Porta F, Notarangelo...

Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells.

We report here homologous recombination (HR)-mediated gene targeting of two different genes in human iPS cells (hiPSCs) and human ES cells (hESCs). HR-mediated correction of a chromosomally integrated mutant GFP reporter gene reaches efficiencies of...

Authors: Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, Chou BK, Chen G, Ye Z, Park IH,...

SST Peer Reviewed

Transcriptional restriction of human immunodeficiency virus type 1 gene expression in undifferentiated primary monocytes

Monocytes are critical precursors of dendritic cells and macrophages, which play an important role in the pathogenesis of human immunodeficiency virus type 1 (HIV-1). HIV-1 postentry infection is blocked in undifferentiated monocytes in vitro, while...

Authors: Dong C, Kwas C, Wu L

Validation of a NAT-based Mycoplasma assay according European Pharmacopoiea.

Eucaryotic expression systems are widely used to produce biologicals for human use, e.g. vaccines, recombinant proteins and monoclonal antibodies. As part of the safety testing the current U.S. Food and Drug Administration (FDA) regulatory guidelines...

Authors: Deutschmann SM, Kavermann H, Knack Y.

SST Peer Reviewed

A FOXO-Pak1 transcriptional pathway controls neuronal polarity

Neuronal polarity is essential for normal brain development and function. However, cell-intrinsic mechanisms that govern the establishment of neuronal polarity remain to be identified. Here, we report that knockdown of endogenous FOXO proteins in...

Authors: de la Torre-Ubieta L, Gaudillière B, Yang Y, Ikeuchi Y, Yamada T, DiBacco S, Stegmüller J, Schüller...

Evaluation of three commercial kits for mycoplasma NAT assays: selection and quality improvement.

Authors: Dorange F, Le Goff F, Dumey N

SST Peer Reviewed

Cross comparison of rapid mycoplasma detection platforms

The use of animal and plant derived raw materials in mammalian cell culture processes may provide a possible route of entry for adventitious contaminants such as mycoplasma. Mycoplasma contaminations of cell culture represent a serious challenge to...

Authors: Lawrence B, Bashiri H, Dehghani H

SST Peer Reviewed

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The Anti-Inflammatory Effects of Adiponectin Are Mediated via a Heme Oxygenase-1–Dependent Pathway in Rat Kupffer Cells

The stargazin-related protein gamma 7 interacts with the mRNA-binding protein heterogeneous nuclear ribonucleoprotein A2 and regulates the stability of specific mRNAs, including CaV2.2

Glucocorticoids sensitize the innate immune system through regulation of the NLRP3 inflammasome.

Severe impairment of IFN-? and IFN-? responses in cells of a patient with a novel STAT1 splicing mutation.

Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells.

Transcriptional restriction of human immunodeficiency virus type 1 gene expression in undifferentiated primary monocytes

Validation of a NAT-based Mycoplasma assay according European Pharmacopoiea.

A FOXO-Pak1 transcriptional pathway controls neuronal polarity

Evaluation of three commercial kits for mycoplasma NAT assays: selection and quality improvement.

Cross comparison of rapid mycoplasma detection platforms