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Methods for siRNA-mediated Reduction of mRNA and Protein Expression in Human Placental Explants, Isolated Primary Cells and Cell Lines

The use of RNA interference (RNAi) to deplete individual proteins from cells or tissue has revolutionised our ability to characterise gene function. The placenta is an attractive target for studies in which the role of specific proteins can be...

Authors: K. Forbes, M. Desforges, R. Garside, J.D. Aplin, and M. Westwood

Distinct roles of heterogenous nuclear ribonuclear protein K and microrna-16 in cyclooxygenase-2 RNA stability induced by S100B, a ligand of the receptor for advanced glycation end products

Advanced glycation end products play major roles in diabetic complications. They act via their receptor RAGE to induce inflammatory genes such as Cycloxygenase-2 (COX-2). We examined the molecular mechanisms by which the RAGE ligand, S100b, induces...

Authors: Shanmugam N, Reddy MA, Natarajan R

Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection

Background: Nucleofection is an emerging technology for delivery of nucleic acids into both the cytoplasm and nucleus of eukaryotic cells with high efficiency. This makes it an ideal technology for gene delivery and siRNA applications. A 96-well...

Authors: Bradburne C, Robertson K, Thach D

SST Peer Reviewed

Preparation of reference strains for validation and comparison of mycoplasma testing methods

AIMS: To optimize growth conditions for preparation of stocks of mycoplasma reference strains to obtain highly viable and disperse samples with low ratios of genomic copy (GC) number to that of colony forming units (CFU). These stocks are required...

Authors: Dabrazhynetskaya A, Volokhov DV, David SW, Ikonomi P, Brewer A, Chang A, Chizhikov V.

SST Peer Reviewed

Immune depletion with cellular mobilization imparts immunoregulation and reverses autoimmune diabetes in nonobese diabetic mice

OBJECTIVE: The autoimmune destruction of beta-cells in type 1 diabetes results in a loss of insulin production and glucose homeostasis. As such, an immense interest exists for the development of therapies capable of attenuating this destructive...

Authors: Parker MJ, Xue S, Alexander JJ, Wasserfall CH, Campbell-Thompson ML, Battaglia M, Gregori S, Mathews...

Optimization of adult sensory neuron electroporation to study mechanisms of neurite growth

The development of eukaryotic transfection technologies has been rapid in recent years, providing the opportunity to better analyze cell-autonomous mechanisms influencing various cellular processes, including cell-intrinsic regulators of regenerative...

Authors: McCall J, Nicholson L, Weidner N, Blesch A.

SST Peer Reviewed

Human induced pluripotent stem cells free of vector and transgene sequences.

Reprogramming differentiated human cells to induced pluripotent stem (iPS) cells has applications in basic biology, drug development, and transplantation. Human iPS cell derivation previously required vectors that integrate into the genome, which can...

Authors: Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA.

SST Peer Reviewed

Vesicular trafficking through cortical actin during exocytosis is regulated by the Rab27a effector JFC1/Slp1 and the RhoA-GAP GMIP

Cytoskeleton remodeling is important for the regulation of vesicular transport associated with exocytosis but a direct association between granular secretory proteins and actin remodeling molecules has not been shown and this mechanism remains...

Authors: Johnson JL, Monfregola J, Napolitano G, Kiosses WB, Catz SD

SST Peer Reviewed

Modulation of Kv3.4 channel N-type inactivation by protein kinase C shapes the action potential in dorsal root ganglion neurons.

Fast inactivation of heterologously expressed Kv3.4 channels is dramatically slowed upon phosphorylation of the channel's N-terminal (N-type) inactivation gate by protein kinase C (PKC). However, the presence and physiological importance of this...

Authors: Ritter DM, Ho C, O'Leary ME, Covarrubias M.

SST Peer Reviewed

Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector.

Induced pluripotent stem (iPS) cells have generated keen interest due to their potential use in regenerative medicine. They have been obtained from various cell types of both mice and humans by exogenous delivery of different combinations of Oct4,...

Authors: Gonzalez F, Barragan Monasterio M, Tiscornia G, Montserrat Pulido N, Vassena R, Batlle Morera L,...

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Methods for siRNA-mediated Reduction of mRNA and Protein Expression in Human Placental Explants, Isolated Primary Cells and Cell Lines

Distinct roles of heterogenous nuclear ribonuclear protein K and microrna-16 in cyclooxygenase-2 RNA stability induced by S100B, a ligand of the receptor for advanced glycation end products

Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection

Preparation of reference strains for validation and comparison of mycoplasma testing methods

Immune depletion with cellular mobilization imparts immunoregulation and reverses autoimmune diabetes in nonobese diabetic mice

Optimization of adult sensory neuron electroporation to study mechanisms of neurite growth

Human induced pluripotent stem cells free of vector and transgene sequences.

Vesicular trafficking through cortical actin during exocytosis is regulated by the Rab27a effector JFC1/Slp1 and the RhoA-GAP GMIP

Modulation of Kv3.4 channel N-type inactivation by protein kinase C shapes the action potential in dorsal root ganglion neurons.

Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector.