Search Results

Tribbles-2 is a novel regulator of inflammatory activation of monocytes

Inflammatory activation of monocytes is an essential part of both innate immune responses and the pathogenesis of conditions such as atherosclerosis. However, the mechanisms which modulate the response of monocytes to inflammatory stimuli are still...

Authors: Eder K, Guan H, Sung HY, Ward J, Angyal A, Janas M, Sarmay G, Duda E, Turner M, Dower SK, Francis...

Efficient non-viral transfection of primary human adult chondrocytes in a high-throughput format

OBJECTIVE: The development of a reliable high-throughput transfection protocol for primary human articular chondrocytes. METHODS: Primary human chondrocytes were isolated from adult knee cartilage by an optimized enzymatic digestion protocol and...

Authors: Haag J, Voigt R, Soeder S, Aigner T

SST Peer Reviewed

Regulation of Myogenic Differentiation by Androgens: Cross-Talk between Androgen Receptor/beta-Catenin and Follistatin/TGF-beta Signaling Pathways

Androgens are important regulators of body composition and promote myogenic differentiation and inhibit adipogenesis of mesenchymal, multipotent cells. Here, we investigated the mechanisms by which androgens induce myogenic differentiation of...

Authors: Singh R, Bhasin S, Braga M, Artaza JN, Pervin S, Taylor WE, Krishnan V, Sinha SK, Rajavashisth TB,...

An efficient method for the long-term and specific expression of exogenous cDNAs in cultured Purkinje neurons.

We present a simple and efficient method for expressing cDNAs in Purkinje neurons (PNs) present in heterogeneous mouse cerebellar cultures. The method combines the transfection of freshly dissociated cerebellar cells via nucleofection with the use of...

Authors: Wagner W, McCroskery S, Hammer JA 3rd.

Nonviral gene delivery of erythropoietin by mesenchymal stromal cells.

Erythropoietin (EPO) acts on erythroblasts in the bone marrow (BM) to stimulate the formation of red blood cells. In this study, we wanted to determine whether BM-derived mesenchymal stromal cells (MSCs) can be used as cellular vehicles to deliver...

Authors: Scheibe F, Gladow N, Mergenthaler P, Tucker AH, Meisel A, Prockop DJ, Priller J.

Proteins involved in cell migration from glioblastoma neurospheres analyzed by overexpression and siRNA-mediated knock-down.

Glioblastoma multiforme (GBM) are the most common malignant brain tumours in adults, characterized by short survival periods of patients. Their aggressive local growth pattern and increased invasiveness, due to a high motility of the tumour cells,...

Authors: Hagemann C, Said HM, Flentje M, Roosen K, Vince GH.

Nucleofection of DCs to generate Multivirus-specific T cells for prevention or treatment of viral infections in the immunocompromised host.

Viral infections cause morbidity and mortality in allogeneic hematopoietic stem cell transplant (HSCT) recipients. To prevent and treat these, we have produced and infused cytotoxic T lymphocytes (CTLs) with specificity for Epstein-Barr virus (EBV),...

Authors: Gerdemann U, Christin AS, Vera JF, Ramos CA, Fujita Y, Liu H, Dilloo D, Heslop HE, Brenner MK,...

c-myb supports erythropoiesis through the transactivation of KLF1 and LMO2 expression.

The c-myb transcription factor is highly expressed in immature hematopoietic cells and down-regulated during differentiation. To define its role during the hematopoietic lineage commitment, we silenced c-myb in human CD34(+) hematopoietic...

Authors: Bianchi E, Zini R, Salati S, Tenedini E, Norfo R, Tagliafico E, Manfredini R, Ferrari S.

Virus-free induction of pluripotency and subsequent excision of reprogramming factors

Reprogramming of somatic cells to pluripotency, thereby creating induced pluripotent stem (iPS) cells, promises to transform regenerative medicine. Most instances of direct reprogramming have been achieved by forced expression of defined factors...

Authors: Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, Woltjen K

SST Peer Reviewed

Stable gene transfer and expression in cord blood-derived CD34+ hematopoietic stem and progenitor cells by a hyperactive Sleeping Beauty transposon system.

Here we report stable gene transfer in cord blood-derived CD34(+) hematopoietic stem cells using a hyperactive nonviral Sleeping Beauty (SB) transposase (SB100X). In colony-forming assays, SB100X mediated the highest efficiency (24%) of stable...

Authors: Xue X, Huang X, Nodland SE, Mátés L, Ma L, Izsvák Z, Ivics Z, LeBien TW, McIvor RS, Wagner JE, Zhou...

SST Peer Reviewed

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Topic

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Tribbles-2 is a novel regulator of inflammatory activation of monocytes

Efficient non-viral transfection of primary human adult chondrocytes in a high-throughput format

Regulation of Myogenic Differentiation by Androgens: Cross-Talk between Androgen Receptor/beta-Catenin and Follistatin/TGF-beta Signaling Pathways

An efficient method for the long-term and specific expression of exogenous cDNAs in cultured Purkinje neurons.

Nonviral gene delivery of erythropoietin by mesenchymal stromal cells.

Proteins involved in cell migration from glioblastoma neurospheres analyzed by overexpression and siRNA-mediated knock-down.

Nucleofection of DCs to generate Multivirus-specific T cells for prevention or treatment of viral infections in the immunocompromised host.

c-myb supports erythropoiesis through the transactivation of KLF1 and LMO2 expression.

Virus-free induction of pluripotency and subsequent excision of reprogramming factors

Stable gene transfer and expression in cord blood-derived CD34+ hematopoietic stem and progenitor cells by a hyperactive Sleeping Beauty transposon system.