Search Results

Multiple Regulatory Steps Control Mammalian Nonmuscle Myosin II Assembly in Live Cells

To better understand the mechanism controlling nonmuscle myosin II (NM-II) assembly in mammalian cells, mutant NM-IIA constructs were created to allow tests in live cells of two widely studied models for filament assembly control. A GFP-NM-IIA...

Authors: Breckenridge MT, Dulyaninova NG, Egelhoff TT

Regulation of cartilage-specific gene expression in human chondrocytes by SirT1 and NAMPT

SSirT1 is an NAD-dependent histone deacetylase that regulates gene expression, differentiation, development and organism life span. Here we investigate the function of SirT1 in human chondrocytes derived from osteoarthritic patients. Elevation of...

Authors: Dvir-Ginzberg M, Gagarina V, Lee EJ, Hall DJ

Different polycomb group CBX family proteins associate with distinct regions of chromatin using nonhomologous protein sequences

Polycomb group proteins are transcriptional repressors recruited to many developmental control genes. The specificity of polycomb group protein targeting is incompletely understood. Subunits of polycomb repressive complexes (PRC) are encoded by...

Authors: Vincenz C, Kerppola TK

Ca2+-regulated Pool of PtdIns(3)P Produced by PI3 kinase-C2alpha on Neurosecretory Vesicles

Phosphatidylinositol-3-phosphate (PtdIns(3)P) is a key player in early endosomal trafficking and is mainly produced by class III PI3-kinase. In neurosecretory cells, class II PI3-kinase C2alpha and its lipid product PtdIns(3)P have recently been...

Authors: Wen PJ, Osborne SL, Morrow IC, Parton RG, Domin J, Meunier FA

BAC transgenesis in human embryonic stem cells as a novel tool to define the human neural lineage.

Human embryonic stem cells (hESCs) have enormous potential for applications in basic biology and regenerative medicine. However, harnessing the potential of hESCs toward generating homogeneous populations of specialized cells remains challenging....

Authors: Placantonakis DG, Tomishima MJ, Lafaille F, Desbordes SC, Jia F, Socci ND, Viale A, Lee H, Harrison...

SST Peer Reviewed

Human hematopoietic stem/progenitor cells modified by zinc-finger nucleases targeted to CCR5 control HIV-1 in vivo

CCR5 is the major HIV-1 co-receptor, and individuals homozygous for a 32-bp deletion in CCR5 are resistant to infection by CCR5-tropic HIV-1. Using engineered zinc-finger nucleases (ZFNs), we disrupted CCR5 in human CD34(+) hematopoietic...

Authors: Holt N, Wang J, Kim K, Friedman G, Wang X, Taupin V, Crooks GM, Kohn DB, Gregory PD, Holmes MC,...

SST Peer Reviewed

Methods for siRNA-mediated reduction of mRNA and protein expression in human placental explants, isolated primary cells and cell lines.

The use of RNA interference (RNAi) to deplete individual proteins from cells or tissue has revolutionised our ability to characterise gene function. The placenta is an attractive target for studies in which the role of specific proteins can be...

Authors: Forbes K, Desforges M, Garside R, Aplin JD, Westwood M.

The control of Ca2+ influx and NFATc3 signaling in arterial smooth muscle during hypertension

Many excitable cells express L-type Ca(2+) channels (LTCCs), which participate in physiological and pathophysiological processes ranging from memory, secretion, and contraction to epilepsy, heart failure, and hypertension. Clusters of LTCCs can...

Authors: Nieves-Cintron M, Amberg GC, Navedo MF, Molkentin JD, Santana LF

Peptide-mediated disruption of calmodulin-cyclin E interactions inhibits proliferation of vascular smooth muscle cells and neointima formation.

RATIONALE: Cell cycle progression in vascular smooth muscle cells (VSMCs) is a therapeutic target for restenosis. OBJECTIVE: Having discovered that calmodulin (CaM)-dependent cyclin E/CDK2 activity underlies Ca(2+)-sensitive G(1)-to-S phase...

Authors: Hui S, Choi J, Zaidi S, Momen A, Steinbach SK, Sadi AM, Ban K, Husain M.

In vitro reprogramming of adult hepatocytes into insulin-producing cells without viral vectors.

The pancreas and the liver share the same endodermal origin. We have been studying whether mature hepatocytes can be induced to differentiate into pancreatic beta-cells by in vitro delivery of transcriptional factors using a non-viral approach. Here...

Authors: Motoyama H, Ogawa S, Kubo A, Miwa S, Nakayama J, Tagawa Y, Miyagawa S.

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