Search Results

Isoelectric focusing in agarose gel for detection of oligoclonal bands in cerebrospinal and other biological fluids

Isoelectric focusing (IEF) coupled with immunodetection (immunofixation or immunoblotting) has become the leading technique for the detection and study of oligoclonal bands (OCBs) in cerebrospinal fluid (CSF) and also is increasingly used in other...

Authors: Csako G.

Widespread deregulation of phosphorylation-based signaling pathways in multiple myeloma cells: opportunities for therapeutic intervention.

Multiple myeloma (MM) is a neoplasm of plasma cell origin that is largely confined to the bone marrow (BM). Chromosomal translocations and other genetic events are known to contribute to deregulation of signaling pathways that lead to transformation...

Authors: Fuhler GM, Diks SH, Peppelenbosch MP, Kerr WG.

SST Peer Reviewed

Relevant shaking stress conditions for antibody preformulation development.

In protein formulation development, shaking stress is often employed to assess the physical stability of antibody formulations against aggregation. Since there are currently no guidelines describing suitable test conditions, very different shaking...

Authors: Eppler A, Weigandt M, Hanefeld A, Bunjes H.

Human neutrophil defensins selectively chemoattract naive T and immature dendritic cells.

Defensins, a family of cationic, structurally related, antimicrobial peptides, contribute to host defense by disrupting the cytoplasmic membrane of microbes. Here we show that human neutrophil defensins selectively induce the migration of human...

Authors: Yang D, Chen Q, Chertov O, Oppenheim JJ.

SST Peer Reviewed

Circulating progenitor cells can be reliably identified on the basis of aldehyde dehydrogenase activity.

OBJECTIVES: Our objective was to develop and assess a novel endogenous progenitor cell (EPC) assay based on aldehyde dehydrogenase (ALDH) activity, and to define the relationship of ALDH-bright (ALDH(br)) cells with previously defined EPCs,...

Authors: Povsic TJ, Zavodni KL, Kelly FL, Zhu S, Goldschmidt-Clermont PJ, Dong C, Peterson ED.

SST Peer Reviewed

An innovative approach for the characterization of the isoforms of a monoclonal antibody product

Protein biopharmaceuticals, such as monoclonal antibodies (mAbs) are widely used for the prevention and treatment of various diseases. The complex and lengthy upstream and downstream production methods of the antibodies make them susceptible to...

Authors: Sundaram S1, Matathia A, Qian J, Zhang J, Hsieh MC, Liu T, Crowley R, Parekh B, Zhou Q.

Acceleration of skeletal muscle regeneration in a rat skeletal muscle injury model by local injection of human peripheral blood-derived CD133-positive cells.

Muscle injuries in sport activities can pose challenging problems in traumatology and sports medicine. The best treatment for muscle injury has not been clearly established except for the conservative treatment that is routinely performed. We...

Authors: Shi M, Ishikawa M, Kamei N, Nakasa T, Adachi N, Deie M, Asahara T, Ochi M.

SST Peer Reviewed

Osteopontin and the C-terminal peptide of thrombospondin-4 compete for CD44 binding and have opposite effects on CD133+ cell colony formation.

BACKGROUND: C21, the C-terminal peptide of thrombospondin-4, has growth promoting activity and was discovered as one of several erythropoietin-dependent endothelial proteins. C21 stimulates red cell formation in anemic mice and is a growth factor...

Authors: Sadvakassova G, Dobocan MC, Congote LF

SST Peer Reviewed

Development of a two-part strategy to identify a therapeutic human bispecific antibody that inhibits IgE receptor signaling

The development of bispecific antibodies as therapeutic agents for human diseases has great clinical potential, but broad application has been hindered by the difficulty of identifying bispecific antibody formats that exhibit favorable...

Authors: Jackman J, Chen Y, Huang A, Moffat B, Scheer JM, Leong SR, Lee WP, Zhang J, Sharma N, Lu Y, Iyer S,...

Construction of a Three-Dimensional Human Skin Model Consisting of Keratinocytes, Dendritic Cells and Fibroblasts and Application of This Model for Alternative Animal Testing of Immune-Sensitizing Compounds

In order to establish in vitro evaluation of the sensitization of human skin, we attempted to make a three-dimensional human skin model consisting of three different cells, dendritic cells, keratinocytes and fibroblasts. The viability of the cells in...

Authors: Uchino T, Ikarashi Y, Tokunaga H

SST Peer Reviewed

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Isoelectric focusing in agarose gel for detection of oligoclonal bands in cerebrospinal and other biological fluids

Widespread deregulation of phosphorylation-based signaling pathways in multiple myeloma cells: opportunities for therapeutic intervention.

Relevant shaking stress conditions for antibody preformulation development.

Human neutrophil defensins selectively chemoattract naive T and immature dendritic cells.

Circulating progenitor cells can be reliably identified on the basis of aldehyde dehydrogenase activity.

An innovative approach for the characterization of the isoforms of a monoclonal antibody product

Acceleration of skeletal muscle regeneration in a rat skeletal muscle injury model by local injection of human peripheral blood-derived CD133-positive cells.

Osteopontin and the C-terminal peptide of thrombospondin-4 compete for CD44 binding and have opposite effects on CD133+ cell colony formation.

Development of a two-part strategy to identify a therapeutic human bispecific antibody that inhibits IgE receptor signaling

Construction of a Three-Dimensional Human Skin Model Consisting of Keratinocytes, Dendritic Cells and Fibroblasts and Application of This Model for Alternative Animal Testing of Immune-Sensitizing Compounds