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How to prolong the shelf-life of supplemented Nucleofector® Solution?

Basically, once the supplement has been added to the Nucleofector® Solution, the shelf-life of the Nucleofector®  Kit is 3 months. However, to prolong the shelf-life of the supplemented Nucleofector® Solution it is possible to mix only what...

Co-culture protocol for Keratinocytes and Fibroblasts (2D) 

If you are searching for a 2D co-culture protocol for matched donor Keratinocytes and Fibroblasts, please find our co-culture protocol on Matched Donor Keratinocytes and Fibroblasts 2D Co-Culture ProtocolFurthermore, here is our White Paper...

What is the diameter of the weldable tube of the LV cartridge (LV unit) ?

PVC tubing (inner diameter: 2.8 mm, outer diameter: 4.0 mm)

Co-culture protocol for Hepatocytes and Non-Parenchymal Cells/NPC (2D) 

If you are searching for a 2D co-culture protocol for Hepatocytes and Non-Parenchymal Cells (NPC), please find our co-culture protocol on Hepatocyte and Non-Parenchymal Cell (NPC) 2D Co-CultureAnd in case you want to work high-throughput, here is...

How do you ship the PyroCell® Monocyte Activation Test (MAT) Kits?

Depending on the scheduled transfer time, cryopreserved goods are either transported in a styrofoam box with dry ice, or in the vapor phase of liquid nitrogen (LN). Upon receiving, ensure immediate storage of components at = -80°C.If dry...

Would a test sample with a high or low pH impair the test results of the PyroCell® Monocyte Activation Test (MAT) Assays?

Yes, as it may impair the recovery of the Positive Product control (PPC control). The IMDM medium has some buffering capacity that might be sufficient to bring a more diluted sample into the correct pH range.

Is it possible to delete users from the 4D-Nucleofector LogWare software?

No. Users can be created and edited but they cannot be deleted. This software is  21CFR Part 11 compliant and those regulations do not permit user deletion.

DonorPlex Pooled Donor Human Hepatocytes: on the certificates of analysis, the percentage of male vs female donors in the pool or caucasian vs asian vs african american donors is indicated. Do the percentages refer to the numbers of donors in the lot or are those the percentage of cells in the pool?

The indicated percentages refer to the numbers of donors in the pool. When pooling, we are targeting multiple variables so the ratio of each donor may be different to achieve a pool that meets the targets specification (for example a specific...

What is the shelf life of the PyroCell® MAT System Kits?

Except for the pMAT cells, the shelf life of each component is indicated on the vial label or the kit box label. The pMAT cells can be used for up to 6 months as of receiving.PyroCell® MAT System Kits component should be used beyond its expiry...
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