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Where are Lonza’s Normal Human Neural Progenitor (NHNP) and Normal Human Astrocytes (NHA) cells isolated from?

Lonza’s Normal Human Neural Progenitor (NHNP) and Normal Human Astrocytes (NHA) cells are primarily isolated from the cerebral cortex (or forebrain from cerebral hemisphere) from fragmented fetal brain tissue (16-20 weeks old).

What is the difference between Lonza’s BEGM™ medium and the Growth Media portion of Lonza’s B-ALI BulletKit™ Medium?

Lonza’s BEGM™ medium and the Growth Media portion of Lonza’s B-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example, cells could be cultured in Lonza’s BEGM™ medium just prior to differentiation using the...

Can I use Nucleofection® Technology for transfecting Plasmodium falciparum?

Yes, but it depends on the platform. We would strongly recommend using the 4D-Nucleofector® or 96-well Shuttle™ System. The successful use of the 96-well Shuttle® System for transfection of P. falciparum has been published by Caro F, Miller...

What is the difference between Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium?

Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example, cells could be cultured in Lonza’s SAGM™ medium just prior to differentiation using the...

Where are Lonza’s aortic adventitial fibroblasts (AoAF) isolated from?

Lonza’s aortic adventitial fibroblasts (AoAF) are isolated from the outer connective tissue layer of the aorta. The aortia is divided into three layers. The tunica intima is the inner endothelial layer, the tunica media is the layers of the smooth...

Where are Lonza’s lung fibroblasts (NHLF and DHLF) isolated from?

Lonza’s lung fibroblasts are not taken from any specific area of the lung but instead are taken from an area that appears to be in good shape and is non-hemorrhagic. The pleural membrane is removed and the peripheral tissue is used. Enzymatic...

Where are Lonza’s dermal fibroblasts (NHDF) isolated from?

Lonza’s offers dermal fibroblasts from both adult and neonatal donors. Lonza’s adult dermal fibroblasts (NHDF-Ad) are usually isolated from tissue obtained from elective cosmetic surgical procedures. Generally, these procedures are abdominoplasties...

What is the purpose of the 5-bromo-2'-deoxyuridine (BrdU) when culturing rat cardiac myocytes?

5-bromo-2'-deoxyuridine (BrdU) is very efficient at inhibiting the proliferation of fibroblasts which are natively present at the time of isolation. Without the addition of the BrdU, the fibroblasts would overtake the culture.

Can an alternate to the 5-bromo-2'-deoxyuridine (BrdU) be used to control the growth of fibroblasts when culturing rat cardiac myocytes?

At this time, we can only recommend the use of BrdU for controlling the growth of fibroblasts.When developing this product, we tried using cytosine beta-arabino furanoside (CAF) to inhibit fibroblast growth. While CAF successfully inhibited the...

Can I still use Clonetics®/Poietics® Basal Media if it froze/partially froze during shipping?

Occasionally, though more frequently as outdoor temperatures begin to drop, media that Lonza recommends storing refrigerated will either completely freeze or partially freeze during shipping. While we do not suggest intentionally freezing and storing...

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