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Do mesenchymal stem cells cultured in TheraPEAK™ Mesenchymal Stem Cell Growth Medium (TheraPeak™ MSCGM™) require an extracellular matrix/plate coating?

While, cells may be able to be cultured in TheraPEAK™ Mesenchymal Stem Cell Growth Medium (TheraPeak™ MSCGM™) without an extracellular matrix/plate coating, for peak performance, a 5ug/mL fibronectin coating should be used. Alternative plate...
Missing: stable linesGuideline

What can I do if there is no Nucleofection® optimized protocol available for my cell of interest?

Lonza is constantly developing new optimized kits and protocols for an increasing number of primary cells and cell lines. In order to obtain the latest information, check our website or contact our Scientific Support Team.We are offering Primary...
Missing: stable linesGuideline

Are ciliated cells present in the SAEC’s?

There are usually ciliated cells present in the SAEC isolations, which shows that the tissue is good and healthy and that everything went well in the isolation process.  However, the cilia do not persist during submers culture...
Missing: stable linesGuideline

Can mycoplasma grow outside of a cell?

This is an area that is up for debate. There is evidence that mycoplasma can survive in the absence of cells, and some may proliferate, but as a rule they are much happier if cells are present.
Missing: stable linesGuideline

Can mycoplasma grow in the absence of cells?

There is evidence to suggest that mycoplasma can survive in the absence of cells, some may even proliferate, but as a rule they are much more viable if cells are present. 
Missing: stable linesGuideline

Are there any recommendations to avoid excessive mortality during T cell Nucleofection®?

Try to keep the DNA amount for Nucleofection® quite low (e.g. 1 µg plasmid DNA per 100 µl reaction) since higher DNA amounts might cause increasing toxicity and mortality. It is also important to use highly purified DNA, e.g. by using Endofree™...
Missing: stable linesGuideline

Can heparin be excluded when culturing endothelial cells? What is the purpose of heparin in the endothelial cell culture media?

...be noted that the Bovine Brain Extract vial in both the EGM™ Media and the EGM™-MV Media contains a small amount of heparin. Please note that Lonza cannot guarantee cell performance if any of the recommended supplements are excluded....
Missing: stable linesGuideline

How are Lonza's mononuclear cells isolated?

Lonza's mononuclear cells are isolated from bone marrow, cord blood, or peripheral blood via density gradient centrifugation
Missing: stable linesGuideline

Where are Lonza's Bronchial Smooth Muscle Cells isolated from?

Lonza’s Bronchial Smooth Muscle Cells (both normal and diseased) are isolated from the bronchial tubes. The region used is around the bifurcation of the bronchial tubes to the lungs and small sections of the bronchia and trachea just off...
Missing: stable linesGuideline

Can calcium influx issues affect viability or differentiation in Stem Cells after Nucleofection®?

When cells are transfected by Nucleofection®, transient pores are generated in the cell membrane. Generally, these pores disappear within 15 minutes of transfection, but if cells are plated immediately after Nucleofection® in medias...
Missing: stable linesGuideline
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