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Export Citations
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For the cultivation of Natural Killer (NK) cells we recommend using the X-VIVO 15 medium.In regard to cytokines, this varies depending upon the application, but many cited publications indicate using IL-2 for activation and longer term culture.
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Publications indicate that IL-2 and IL-21 can stimulate NK cell proliferation in addition to using a human serum supplemented medium.
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Most Clonetics™ Cells do not require an extracellular matrix when grown on tissue culture treated plastic – flasks, dishes, well plates (with the exception of hepatocytes, and the rat/mouse neuronal and dorsal root ganglion neurons, rat cardiac...
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We have seen on multiple occasions that the effects of centrifugation can be significantly more damaging than the effects of residual DMSO on primary cells. As such, unless specifically noted in the recommended culture protocol, instead of...
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Resting Natural Killer cells grow in suspension, however, when IL-2 is added to the culture, they will adhere.
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Clonetics™ Cells will not be confluent on day one. After 24 hours, you will see few attached cells per field under the microscope. Aspirate off the media and the floating cells and replace with fresh, pre-warmed media. Those attached cells will begin...
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All Clonetics™ Cells are primary cells with a finite life span. Most Clonetics™ Cells are guaranteed for 10 – 15 additional population doublings, which equates to about 2 – 3 passages. As cell death begins to occur, morphology and cell performance...
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TGF-B3 has worked best in our hands so that is what we recommend.
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Cryopreservation may compromise cell quality and performance. Lonza CANNOT guarantee performance of Clonetics™ & Poietics™ Cells that have been cryopreserved outside of Lonza. To avoid loss of cells and forfeiture of your warranty, we recommend...
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That will depend on the type of medium used. If you are using our MSCGM™ BulletKit™ PT-3001, we do not recommend using a matrix with the cells as it might induce differentiation and make the cells difficult to detach from the culture vessel. If you...
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