Data Type
Cell Information
(858)
Citations
(4968)
FAQ
(515)
Culture Media
(61)
Category
Transfection
(3843)
Primary Cells and Media
(1963)
Serum-free and Speciality Media
(1170)
Classical Media and Reagents
(1054)
Cell Lines and Primary Cancer Cells
(644)
Bioassay
(117)
Electrophoreses and Analysis
(99)
3D Cell Culture
(91)
Laboratory Instrumentation
(75)
Endotoxin Detection
(66)
Mycoplasma Detection and Prevention
(36)
Protozoa
(16)
Live Cell Imaging
(7)
Cell Services
(4)
Uncategorized
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
+ Show All
Research Area
Cancer Research/Cell Biology
(2175)
Basic Research
(1648)
Immunotherapy / Hematology
(1590)
Neurobiology
(617)
Stem Cells
(385)
Cardiovascular
(318)
Gene Expression
(278)
Dermatology/Tissue Engineering
(265)
Molecular Biology
(179)
Gastroenterology
(139)
Regenerative medicine
(96)
Drug Discovery
(94)
Endotoxin Testing
(90)
Toxicology
(81)
Respiratory Research
(71)
Parasitology
(69)
Uncategorized
(48)
+ Show All
Platform
Nucleofector® I/II/2b
(3039)
4D-Nucleofector® X-Unit
(596)
4D-Nucleofector® 96-well Systems
(245)
4D-Nucleofector® Y-Unit
(41)
384-well HT Nucleofector® System
(17)
4D-Nucleofector® LV-Unit
(13)
Topic
Signal transduction
(1269)
Microscopy of nucleofected cells
(747)
Cellular Function
(614)
Diseases (e.g. HIV)
(601)
Cell morphology/cytoskeleton
(405)
Apoptosis
(363)
Luciferase
(330)
Genome Editing
(312)
Intercellular interaction/communication
(293)
Differentiation of nucleofected cells
(230)
Expression of dominant-negative protein
(198)
Gene therapy
(180)
Hereditary diseases
(100)
Comparison to other transfection methods
(78)
Promoter analysis
(74)
RNA transfection
(57)
Antisense
(56)
Transplantation of nucleofected cells
(32)
Virus production
(19)
Infection of Nucleofected cells
(17)
Tissue transfection
(7)
Cryopreservation
(5)
+ Show All
Species
human
(524)
mouse
(183)
rat
(66)
monkey
(15)
bovine
(11)
hamster
(10)
unicellular
(9)
porcine
(8)
chicken
(7)
canine
(6)
rabbit
(3)
baboon
(2)
cell hybrid
(2)
fish
(2)
spodoptera frugiperda (army fall worm)
(2)
Trichopulsia ni (Cabbage Looper)
(1)
drosophila melanogaster (fruit fly)
(1)
equine
(1)
feline
(1)
ferret
(1)
potoroo
(1)
sheep
(1)
+ Show All
Characteristic
Adherent
(457)
Suspension
(400)
Format
Bullet Kit
(46)
Lonza Specialty Media
(14)
Classical Media
(1)
Origin
Animal
(42)
Non-Animal
(10)
6402 results sorted by
relevance
alphabetical
newest first
oldest first
Export Citations
C
a
n
I
c
o
-
t
r
a
n
s
f
e
c
t
o
l
i
g
o
n
u
c
l
e
o
t
i
d
e
s
a
n
d
p
l
a
s
m
i
d
s
w
i
t
h
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
T
e
c
h
n
o
l
o
g
y
?
Yes. As the same protocol applies for any nucleic acid substrate (vectors or oligonucleotides) you can easily perform co-transfections either for transfection control or rescue experiments.
C
a
n
I
i
n
t
e
g
r
a
t
e
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
D
e
v
i
c
e
i
n
t
o
a
l
i
q
u
i
d
h
a
n
d
l
i
n
g
s
y
s
t
e
m
?
Yes. The 96-well Shuttle® Device is designed in such a way that it is addressable by robot grippers. Furthermore, the software contains the required interface to be connected with the LHS software. However, the practical implementation for each...
D
o
e
s
t
h
e
G
F
P
s
e
q
u
e
n
c
e
i
n
t
h
e
p
m
a
x
G
F
P
™
c
o
n
t
r
o
l
p
l
a
s
m
i
d
h
a
s
a
s
t
o
p
c
o
d
o
n
?
Yes. The coding sequence ends with a stop codon (TGA/UGA).
C
a
n
I
u
s
e
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
2
b
S
o
l
u
t
i
o
n
s
a
n
d
O
p
t
i
m
i
z
e
d
P
r
o
t
o
c
o
l
s
w
i
t
h
t
h
e
4
D
-
N
u
c
l
e
o
f
e
c
t
o
r
®
X
-
U
n
i
t
o
r
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
S
y
s
t
e
m
?
No. The 4D-Nucleofector and 96-well Shuttle® System uses specifically optimized electrical parameters and solutions. Five different 4D-Nucleofector or 96-well Shuttle® Solutions are available for primary cells. For cell lines, three...
W
h
a
t
a
n
t
i
b
o
d
i
e
s
c
a
n
b
e
u
s
e
d
a
g
a
i
n
s
t
t
h
e
G
F
P
p
r
o
d
u
c
e
d
b
y
p
m
a
x
G
F
P
™
a
n
d
p
m
a
x
F
P
™
-
G
r
e
e
n
v
e
c
t
o
r
s
?
W
h
a
t
a
n
t
i
b
o
d
i
e
s
c
a
n
b
e
u
s
e
d
t
o
d
e
t
e
c
t
d
e
n
a
t
u
r
e
d
G
F
P
?
W
h
a
t
a
n
t
i
b
o
d
i
e
s
c
a
n
b
e
u
s
e
d
t
o
d
e
t
e
c
t
t
h
e
R
F
P
e
x
p
r
e
s
s
e
d
b
y
p
m
a
x
F
P
-
R
e
d
?
Most of the commercial antibodies for GFP do not detect GFP expressed from pmaxGFP or pmaxFP-Green vectors. The origin of the protein expressed by the pmaxGFP control plasmid and pmaxFP-Green is from the green fluorescent protein CopGFP, cloned from...
W
h
a
t
k
i
n
d
o
f
c
u
v
e
t
t
e
s
c
a
n
o
n
e
u
s
e
f
o
r
t
h
e
t
r
a
n
s
f
o
r
m
a
t
i
o
n
o
f
b
a
c
t
e
r
i
a
u
s
i
n
g
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
I
/
I
I
/
2
b
?
We recommend using cuvettes with the 0.1 cm gap width.
C
a
n
I
u
s
e
a
N
u
c
l
e
o
f
e
c
t
o
r
®
I
o
r
N
u
c
l
e
o
f
e
c
t
o
r
®
2
b
D
e
v
i
c
e
t
o
g
e
t
h
e
r
w
i
t
h
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
D
e
v
i
c
e
?
No, the 96-well Shuttle® only works in conjunction with the Nucleofector® IIs Device or our 4D-Nucleofector® Device.
W
h
y
d
o
e
s
L
o
n
z
a
r
e
c
o
m
m
e
n
d
u
s
i
n
g
n
o
n
-
v
e
n
t
i
l
a
t
e
d
c
a
p
s
w
h
e
n
c
u
l
t
u
r
i
n
g
i
n
s
e
c
t
c
e
l
l
s
?
The reason is that insect cells are often incubated in very simple incubators that are not humidified (and without CO2 at 24°C to 28°C). Insect media typically do not need CO2 for buffering pH, so gas exchange is not required. To avoid evaporation of...
W
h
a
t
i
s
t
h
e
a
d
v
a
n
t
a
g
e
o
f
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
T
e
c
h
n
o
l
o
g
y
o
v
e
r
c
o
m
m
o
n
e
l
e
c
t
r
o
p
o
r
a
t
i
o
n
(
s
y
s
t
e
m
s
)
?
High cell viability and high transfection efficiency with the Nucleofection® Technology are the most prominent features. Nucleofection®, i.e., the transfer of DNA into the nucleus and not only the cytosol, is essential when working with...
W
h
a
t
i
s
t
h
e
r
a
t
i
o
o
f
S
u
p
p
l
e
m
e
n
t
t
o
N
u
c
l
e
o
f
e
c
t
o
r
®
S
o
l
u
t
i
o
n
?
The ratio is 1:4,5 (500 µL of supplement is added to 2.25 mL of Nucleofector® Solution).For a single 100 µL reaction, use 18 µL of supplement plus 82 µL of solution to make the 100 µL.
PREVIOUS
PAGE
476
PAGE
477
PAGE
478
PAGE
479
PAGE
480
PAGE
481
PAGE
482
PAGE
483
PAGE
484
NEXT