Search Results

Involvement of periostin in regression of hyaloidvascular system during ocular development

PURPOSE: A timely regression of the hyaloid vascular system (HVS) is required for the normal ocular development. Although macrophages have a critical role in this process, the exact mechanism remains undetermined. Periostin is a matricellular...

Authors: Arima M, Yoshida S, Nakama T, Ishikawa K, Nakao S, Yoshimura T, Asato R, Sassa Y, Kita T, Enaida H,...

DICE, an efficient system for iterative genomic editing in human pluripotent stem cells.

To reveal the full potential of human pluripotent stem cells, new methods for rapid, site-specific genomic engineering are needed. Here, we describe a system for precise genetic modification of human embryonic stem cells (ESCs) and induced...

Authors: Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schüle B, Chen-Tsai Y, Calos MP.

SST Peer Reviewed

Genome-wide binding and transcriptome analysis of human farnesoid x receptor in primary human hepatocytes

BACKGROUND & AIMS: Farnesoid X receptor (FXR, NR1H4) is a ligand-activated transcription factor, belonging to the nuclear receptor superfamily. FXR is highly expressed in the liver and is essential in regulating bile acid homeostasis. FXR...

Authors: Zhan L, Liu HX, Fang Y, Kong B, He Y, Zhong XB, Fang J, Wan YJ, Guo GL.

Identification and functional characterization of the iron-dependent regulator (IdeR) of Mycobacterium avium subsp. paratuberculosis

Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of Johne's disease in cattle and sheep, has unique iron requirements in that it is mycobactin-dependent for cultivation in vitro. The iron-dependent regulator (IdeR) is a...

Authors: Janagama HK, Senthilkumar TM, Bannantine JP, Rodriguez GM, Smith I, Paustian ML, McGarvey JA,...

Calcitriol May Down-Regulate mRNA Over-Expression of Toll-Like Receptor-2 and -4, LL-37 and Proinflammatory Cytokines in Cultured Human Keratinocytes

Although vitamin D analogs have been used in the topical treatment of psoriasis, their mechanisms of action are not well understand. Calcitriol, the hormonally active vitamin D3 metabolite, has been demonstrated to exert immunomodulatory effects in...

Authors: Jeong MS, Kim JY, Lee HI, Seo SJ.

Optimization of scarless human stem cell genome editing.

Efficient strategies for precise genome editing in human-induced pluripotent cells (hiPSCs) will enable sophisticated genome engineering for research and clinical purposes. The development of programmable sequence-specific nucleases such as...

Authors: Yang L, Guell M, Byrne S, Yang JL, De Los Angeles A, Mali P, Aach J, Kim-Kiselak C, Briggs AW, Rios...

SST Peer Reviewed

Comprehensive measurement of respiratory activity in permeabilized cells using extracellular flux analysis.

Extracellular flux (XF) analysis has become a mainstream method for measuring mitochondrial function in cells and tissues. Although this technique is commonly used to measure bioenergetics in intact cells, we outline here a detailed XF protocol for...

Authors: Salabei JK, Gibb AA, Hill BG

SST Peer Reviewed

Stem/progenitor cells in closed suction drainage fluid after hip arthroplasty

BACKGROUND AND PURPOSE: Drainage after surgery is commonly used, and the contents are generally discarded as clinical waste. We analyzed closed suction drainage fluid from hip arthroplasty patients to determine whether any multipotent stem cells...

Authors: Sera S, Ishikawa M, Adachi N, Yasunaga Y, Ochi M.

Intracellular Carbonic Anhydrase Activity Sensitizes Cancer Cell pH-signaling to Dynamic Changes in CO2 Partial Pressure

Carbonic anhydrase (CA) enzymes catalyze chemical equilibration between CO2, HCO3 - and H+. Intracellular CAs (CAi) are present in certain types of cancer and some evidence suggests that low levels correlate with disease severity. However, their...

Authors: Hulikova A, Aveyard N, Harris A, Vaughan-Jones R, Swietach P

Hypoxia-mediated Up-regulation of Metastatic Genes in Human Tongue Oral Squamous Cell Carcinoma: [Part I] Urokinase Plasminogen Activator [uPA]

Background: Most human tumors develops regions of chronically or transient hypoxic cells during growth. The effect of hypoxia on tumor tissue has a strong impact on tumor cell migration and metastatic behavior. uPA a major member plasminogen...

Authors: Khaled M. Mohamed, BDS, MDS, PhD.

Data Type

Category

Research Area

Platform

Topic

Species

Characteristic

Format

Origin

Involvement of periostin in regression of hyaloidvascular system during ocular development

DICE, an efficient system for iterative genomic editing in human pluripotent stem cells.

Genome-wide binding and transcriptome analysis of human farnesoid x receptor in primary human hepatocytes

Identification and functional characterization of the iron-dependent regulator (IdeR) of Mycobacterium avium subsp. paratuberculosis

Calcitriol May Down-Regulate mRNA Over-Expression of Toll-Like Receptor-2 and -4, LL-37 and Proinflammatory Cytokines in Cultured Human Keratinocytes

Optimization of scarless human stem cell genome editing.

Comprehensive measurement of respiratory activity in permeabilized cells using extracellular flux analysis.

Stem/progenitor cells in closed suction drainage fluid after hip arthroplasty

Intracellular Carbonic Anhydrase Activity Sensitizes Cancer Cell pH-signaling to Dynamic Changes in CO2 Partial Pressure

Hypoxia-mediated Up-regulation of Metastatic Genes in Human Tongue Oral Squamous Cell Carcinoma: [Part I] Urokinase Plasminogen Activator [uPA]