Search Results

DICE, an efficient system for iterative genomic editing in human pluripotent stem cells.

To reveal the full potential of human pluripotent stem cells, new methods for rapid, site-specific genomic engineering are needed. Here, we describe a system for precise genetic modification of human embryonic stem cells (ESCs) and induced...

Authors: Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schüle B, Chen-Tsai Y, Calos MP.

SST Peer Reviewed

Optimization of scarless human stem cell genome editing.

Efficient strategies for precise genome editing in human-induced pluripotent cells (hiPSCs) will enable sophisticated genome engineering for research and clinical purposes. The development of programmable sequence-specific nucleases such as...

Authors: Yang L, Guell M, Byrne S, Yang JL, De Los Angeles A, Mali P, Aach J, Kim-Kiselak C, Briggs AW, Rios...

SST Peer Reviewed

A simplified method for the generation of human osteoclasts in vitro

Osteoclasts are large, multinucleated cells responsible for the resorption of mineralized bone matrix. These cells are critical players in the bone turnover involved in bone homeostasis. Osteoclast activity is connected to the establishment and...

Authors: Cody JJ, Rivera AA, Liu J, Liu JM, Douglas JT, Feng X.

A novel strategy for enrichment and isolation of osteoprogenitor cells from induced pluripotent stem cells based on surface marker combination

In this study, we developed a new method to stimulate osteogenic differentiation in tissue-nonspecific alkaline phosphatase (TNAP)-positive cells liberated from human induced pluripotent stem cells (hiPSCs)-derived embryoid bodies (EBs) with 14 days...

Authors: Ochiai-Shino H, Kato H, Sawada T, Onodera S, Saito A, Takato T, Shibahara T, Muramatsu T, Azuma T

Comprehensive measurement of respiratory activity in permeabilized cells using extracellular flux analysis.

Extracellular flux (XF) analysis has become a mainstream method for measuring mitochondrial function in cells and tissues. Although this technique is commonly used to measure bioenergetics in intact cells, we outline here a detailed XF protocol for...

Authors: Salabei JK, Gibb AA, Hill BG

SST Peer Reviewed

Phosphoinositide 3-Kinase (PI3K) Activation is Differentially Regulated during Osteogenesis induced by TGF- 1 and BMP-2/BMP-7

Periodontal ligament (PDL) cells are comprised of heterogenous cell populations including mesenchymal stem cells and osteogenic progenitor cells. We previously reported that differentiation of human PDL cells into osteoblasts requires...

Authors: Haruto Yamashita, Hiromi Ochiai, Akiko Saito, Seikou Shintani and Toshifumi Azuma

Characterization of autologous mesenchymal stem cell-derived neural progenitors as a feasible source of stem cells for central nervous system applications in multiple sclerosis

Bone marrow mesenchymal stem cell-derived neural progenitors (MSC-NPs) are a potential therapeutic source of cells that have been shown to be efficacious in a preclinical model of multiple sclerosis (MS). To examine the feasibility of using MSC-NPs...

Authors: Harris VK, Faroqui R, Vyshkina T, Sadiq SA.

Intracellular Carbonic Anhydrase Activity Sensitizes Cancer Cell pH-signaling to Dynamic Changes in CO2 Partial Pressure

Carbonic anhydrase (CA) enzymes catalyze chemical equilibration between CO2, HCO3 - and H+. Intracellular CAs (CAi) are present in certain types of cancer and some evidence suggests that low levels correlate with disease severity. However, their...

Authors: Hulikova A, Aveyard N, Harris A, Vaughan-Jones R, Swietach P

Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane

In the case of melanoma, advances in therapies are slow, which raises the need to evaluate new therapeutic strategies and natural products with potential cancer cell inhibiting effect. Pseudofactin II (PFII), a novel cyclic lipopeptide biosurfactant...

Authors: Janek T, Krasowska A, Radwanska A, Lukaszewicz M.

Bone progenitors produced by direct osteogenic differentiation of the unprocessed bone marrow demonstrate high osteogenic potential in vitro and in vivo

Tissue-engineered bone grafts seeded with mesenchymal stem cells (MSCs) have been sought as a replacement for bone grafts currently used for bone repair. For production of osteogenic constructs, MSCs are isolated from bone marrow (BM) or other...

Authors: Ginis I, Weinreb M, Abramov N, Shinar D, Merchav S, Schwartz A, Shirvan M.

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DICE, an efficient system for iterative genomic editing in human pluripotent stem cells.

Optimization of scarless human stem cell genome editing.

A simplified method for the generation of human osteoclasts in vitro

A novel strategy for enrichment and isolation of osteoprogenitor cells from induced pluripotent stem cells based on surface marker combination

Comprehensive measurement of respiratory activity in permeabilized cells using extracellular flux analysis.

Phosphoinositide 3-Kinase (PI3K) Activation is Differentially Regulated during Osteogenesis induced by TGF- 1 and BMP-2/BMP-7

Characterization of autologous mesenchymal stem cell-derived neural progenitors as a feasible source of stem cells for central nervous system applications in multiple sclerosis

Intracellular Carbonic Anhydrase Activity Sensitizes Cancer Cell pH-signaling to Dynamic Changes in CO2 Partial Pressure

Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane

Bone progenitors produced by direct osteogenic differentiation of the unprocessed bone marrow demonstrate high osteogenic potential in vitro and in vivo