Search Results

PKCdelta acts upstream of SPAK in the activation of NKCC1 by hyperosmomtic stress in airway epithelial cells

Airway epithelial Na-K-2Cl (NKCC1) cotransport is activated through hormonal stimulation and hyperosmotic stress via a PKCdelta-mediated intracellular signalling pathway. Downregulation of PKCdeltaprevents activation of NKCC1 expressed in Calu-3...

Authors: Smith L, Smallwood N, Altman A, Liedtke CM

Molecular dissection of the human alpha 2-macroglobulin subunit reveals domains with antagonistic activities in cell signaling

alpha(2)-Macroglobulin (alpha(2)M) is a plasma protease inhibitor, which reversibly binds growth factors and, in its activated form, binds to low density lipoprotein receptor-related protein (LRP-1), an endocytic receptor with cell-signaling...

Authors: Mantuano E, Mukandala G, Li X, Campana WM, Gonias SL

Orphan nuclear receptor NR4A2 expressed in T cells from multiple sclerosis mediates production of inflammatory cytokines

Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS) mediated by Th17 and Th1 cells. DNA microarray analysis previously showed that NR4A2, an orphan nuclear receptor, is strongly up-regulated in the peripheral blood T...

Authors: Doi Y, Oki S, Ozawa T, Hohjoh H, Miyake S, Yamamura T

The benefit of docosahexanoic acid on the migration of vascular smooth muscle cells is partially dependent on Notch regulation of MMP-2/-9

The Notch pathway is involved in the regulation of the migratory/proliferative phenotype acquired by vascular smooth muscle cells (VSMCs) in the pro-inflammatory context of vascular diseases. Here, we investigated whether docosahexaenoic acid (DHA),...

Authors: Delbosc S, Glorian M, Le Port AS, Bereziat G, Andreani M, Limon I

Crosstalk between expression of the HTLV-1 Tax transactivator and the oncogenic bHLH transcription factor TAL1

The Human T cell Leukemia Virus type 1 (HTLV-1) Tax transactivator is known to induce or repress various cellular genes, several of them encoding transcription factors. As Tax is known to deregulate various bHLH factors, we looked more specifically...

Authors: Terme JM, Wencker M, Favre-Bonvin A, Bex F, Gazzolo L, Duc Dodon M, Jalinot P

Molecular and Genetic Analysis of Condensin Function in Vertebrate Cells

We engineered mutants into residues of SMC2 to dissect the role of ATPase function in the condensin complex. These residues are predicted to be involved in ATP binding or hydrolysis and in the Q-loop, which is thought to act as a mediator of...

Authors: Hudson DF, Ohta S, Freisinger T, Macisaac F, Sennels L, Alves F, Lai F, Kerr A, Rappsilber J,...

STAT5b, c-Src, and EGFR Signaling Play Integral Roles in Estrogen-Stimulated Proliferation of ER-Positive Breast Cancer Cells

17beta-estradiol (E2) acts through the estrogen receptor alpha (ERalpha) to stimulate breast cancer proliferation. Here, we investigated the functional relationship between ERalpha and STAT5b activity in ER+ MCF-7 and T47D human breast cancer cells...

Authors: Fox EM, Bernaciak TM, Wen J, Weaver AM, Shupnik MA, Silva CM

Molecular Dissection of the Structural Machinery Underlying the Tissue-invasive Activity of Membrane Type-1 Matrix Metalloproteinase

Membrane type-I matrix metalloproteinase (MT1-MMP) drives cell invasion through three-dimensional (3-D) extracellular matrix (ECM) barriers dominated by type I collagen or fibrin. Based largely on analyses of its impact on cell function under 2-D...

Authors: Li XY, Ota I, Yana I, Sabeh F, Weiss SJ

Mechanisms of antileukemic activity of the novel Bcl-2 homology domain-3 mimetic GX15-070 (obatoclax).

In this study, we investigated the mechanism of apoptosis induction of obatoclax (GX15-070), a novel Bcl-2 homology domain-3 (BH3) mimetic, in acute myeloid leukemia (AML) cell lines and primary AML samples. Obatoclax inhibited cell growth of HL-60,...

Authors: Konopleva M, Watt J, Contractor R, Tsao T, Harris D, Estrov Z, Bornmann W, Kantarjian H, Viallet J,...

Orexins stimulate steroidogenic acute regulatory (StAR) protein expression through multiple signalling pathways in human adrenal H295R cells

Orexins mediate a variety of physiological processes including feeding behaviour, the circadian pathway and cortisol secretion. Steroidogenesis is regulated by a variety of neuropeptides and one of the key rate-limiting steps is cholesterol transport...

Authors: Ramanjaneya M, Conner AC, Chen J, Stanfield PR, Randeva HS

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