Search Results

Regulation of RKIP binding to the N-region of the Raf-1 kinase

The Raf kinase inhibitory protein (RKIP) binds to Raf-1 interfering with binding of the MEK substrate and potentially also Raf-1 activation. In response to mitogen stimulation RKIP dissociates from Raf-1 and later re-associates. Here, using a...

Authors: Park S, Rath O, Beach S, Xiang X, Kelly SM, Luo Z, Kolch W, Yeung KC

A novel CCCH-Zinc finger protein family regulates proinflammatory activation of macrophages

Activated macrophages play an important role in many inflammatory diseases. However, the molecular mechanisms controlling macrophage activation are not completely understood. Here we report that a novel CCCH zinc finger protein family MCPIP1, 2, 3...

Authors: Liang J, Wang J, Azfer A, Song W, Tromp G, Kolattukudy PE, Fu M

The constitutively active orphan G-protein coupled receptor GPR39 protects from cell death by increasing secretion of pigment-epithelial derived growth factor PEDF

GPR39 is a constitutively active orphan G-protein-coupled receptor capable of increasing serum-response-element mediated transcription. We found GPR39 to be upregulated in a hippocampal cell line resistant against diverse stimulators of cell death...

Authors: Dittmer S, Sahin M, Pantlen A, Saxena A, Toutzaris D, Pina AL, Geerts A, Golz S, Methner A

Regulating gene expression in human leukemia cells using light-activated oligodeoxynucleotides

Light-activated antisense oligodeoxynucleotides (asODNs) were developed to control the degradation of target mRNA in living cells by RNase H. A 20-mer asODN previously shown to target c-myb, a hematopoietic transcription factor, was covalently...

Authors: Tang X, Swaminathan J, Gewirtz AM, Dmochowski IJ

Identification of a novel sn-glycerol-3-phosphate acyltransferase isoform, GPAT4 as the enzyme deficient in Agpat6-/- mice

Elucidation of the metabolic pathways of triacylglycerol synthesis is critical to the understanding of chronic metabolic disorders such as obesity, cardiovascular disease, and diabetes. Glycerol-sn-3-phosphate acyltransferase (GPAT) and...

Authors: Nagle CA, Vergnes L, Dejong H, Wang S, Lewin TM, Reue K, Coleman RA

Lyn-coupled LacCer-enriched lipid rafts are required for CD11b/CD18-mediated neutrophil phagocytosis of nonopsonized microorganisms

The integrin CD11b/CD18 plays a central role in neutrophil phagocytosis. Although CD11b/CD18 binds a wide range of ligands, including C3bi and beta-glucan, and transmits outside-in signaling, the mechanism of this signaling responsible for...

Authors: Nakayama H, Yoshizaki F, Prinetti A, Sonnino S, Mauri L, Takamori K, Ogawa H, Iwabuchi K

How cells tiptoe on adhesive surfaces before sticking

Cell membranes are studded with protrusions that were thoroughly analyzed with electron microscopy. However, the nanometer-scale three-dimensional motions generated by cell membranes to fit the topography of foreign surfaces and initiate adhesion...

Authors: Pierres A, Benoliel AM, Touchard D, Bongrand P

Pyramidal neuron polarity axis is defined at the bipolar stage

In situ observations of the development of hippocampal and cortical neurons indicate that final axon-dendrite identity is defined at the time of generation of the first two, oppositely positioned, neurites. Quite differently, in vitro studies...

Authors: Calderon de Anda F, Gärtner A, Tsai LH, Dotti CG

HIV-1 Nef disrupts the podocyte actin cytoskeleton by interacting with diaphanous interacting protein (DIP)

The HIV-1 protein Nef's ability to induce cytoskeleton changes in infected host cells is a key event in viral replication. In renal podocytes, we found that Nef induced loss of stress fibers and increased lamellipodia, pathological changes leading to...

Authors: Lu TC, He JC, Wang Z, Feng X, Fukumi-Tominaga T, Chen N, Xu J, Iyengar R, Klotman PE

A function retained by the common mutant CLN3 protein is responsible for the late onset of juvenile neuronal ceroid lipofuscinosis

The neuronal ceroid lipofuscinoses (NCLs) are common neurodegenerative disorders of childhood and are classified as lysosomal storage diseases since affected cells exhibit lysosomes containing ceroid and lipofuscin-like material. CLN3 is the most...

Authors: Kitzmuller C, Haines RL, Codlin S, Cutler DF, Mole SE

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