Search Results

The clustering of GABA(A) receptor subtypes at inhibitory synapses is facilitated via the direct binding of receptor alpha 2 subunits to gephyrin

Classical benzodiazepine sensitive GABA(A) receptor subtypes, the major mediators of fast synaptic inhibition in the brain are heteropentamers that can be assembled from alpha1-3/5, beta1-3, and gamma2 subunits, but how neurons orchestrate their...

Authors: Tretter V, Jacob TC, Mukherjee J, Fritschy JM, Pangalos MN, Moss SJ

p21 Waf1/Cip1 expression by curcumin in U-87MG human glioma cells: role of early growth response-1 expression

Curcumin, a natural compound, is a well-known chemopreventive agent with potent anticarcinogenic activity in a wide variety of tumor cells. Curcumin inhibits cancer cell proliferation in part by suppressing cyclin D1 and inducing expression of the...

Authors: Choi BH, Kim CG, Bae YS, Lim Y, Lee YH, Shin SY

Survival signaling by C-RAF: mitochondrial ROS and Ca2+ are critical targets

Survival signaling by RAF occurs through largely unknown mechanisms. Here we provide evidence for the first time that RAF controls cell survival by maintaining permissive levels of mitochondrial reactive oxygen species (ROS) and Ca(2+). IL-3...

Authors: Kuznetsov AV, Smigelskaite J, Doblander C, Janakiraman M, Hermann M, Wurm M, Scheidl S, Sucher R,...

Selective inhibition of histone deacetylase 2 silences progesterone receptor-mediated signaling

Several histone deacetylases (HDAC) are involved in estrogen receptor (ER)-mediated gene transactivation, and HDAC inhibitors have been reported to restore sensitivity to antihormonal therapy. The modulation of ER is the most promising approach to...

Authors: Bicaku E, Marchion DC, Schmitt ML, Münster PN

Nucleofection is a highly effective gene transfer technique for human melanoma cell lines

Despite the increasing use of gene transfer strategies in the study of cellular and molecular biology, melanoma cells have remained difficult to transfect in a safe, efficient, and reproducible manner. In the present study, we report the successful...

Authors: Han SY, Gai W, Yancovitz M, Osman I, Di Como CJ, Polsky D

SST Peer Reviewed

Etk/BMX, a Btk Family Tyrosine Kinase, and Mal Contribute to the Cross-Talk between MyD88 and FAK Pathways

MyD88 and focal adhesion kinase (FAK) are key adaptors involved in signaling downstream of TLR2, TLR4, and integrin alpha(5)beta(1), linking pathogen-associated molecule detection to the initiation of proinflammatory response. The MyD88 and integrin...

Authors: Semaan N, Alsaleh G, Gottenberg JE, Wachsmann D, Sibilia J

Nucleofection Mediates High-efficiency Stable Gene Knockdown and Transgene Expression in Human Embryonic Stem Cells

High-efficiency genetic modification of human embryonic stem (hES) cells would enable manipulation of gene activity, routine gene targeting, and development of new human disease models and treatments. Chemical transfection, nucleofection, and...

Authors: Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ

SST Peer Reviewed

Ceramide triggers budding of exosome vesicles into multivesicular endosomes

Intraluminal vesicles of multivesicular endosomes are either sorted for cargo degradation into lysosomes or secreted as exosomes into the extracellular milieu. The mechanisms underlying the sorting of membrane into the different populations of...

Authors: Trajkovic K, Hsu C, Chiantia S, Rajendran L, Wenzel D, Wieland F, Schwille P, Brügger B, Simons M

Restricted collision coupling of the A2A-receptor revisited: Evidence for physical separation of two signaling cascades

The A(2A)-adenosine receptor is a prototypical G(s) protein-coupled receptor, but stimulates MAP-kinase/erk in a Gs-independent way. The A(2A)-receptor has long been known to undergo restricted collision coupling with G(s); the mechanistic basis for...

Authors: Charalambous C, Gsandtner I, Keuerleber S, Milan-Lobo L, Kudlacek O, Freissmuth M, Zezula J

Interleukin-1 (IL-1) induces the Lys63-linked polyubiquitination of IL-1 receptor-associated kinase 1 to facilitate NEMO binding and the activation of IkappaBalpha kinase

Interleukin 1 (IL-1) has been reported to stimulate the polyubiquitination and disappearance of IL-1 receptor-associated kinase 1 (IRAK1) within minutes. It has been thought that the polyubiquitin chains attached to IRAK1 are linked via Lys48 of...

Authors: Windheim M, Stafford M, Peggie M, Cohen P

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