Search Results

Airway epithelial cells synthesize the lipid mediator 5-oxo-ETE in response to oxidative stress

5-Oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE) is a potent eosinophil chemoattractant that is synthesized from the 5-lipoxygenase product 5S-hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE) by the NADP+-dependent enzyme 5-hydroxyeicosanoid...

Authors: Erlemann KR, Cossette C, Gravel S, Lesimple A, Lee GJ, Saha G, Rokach J, Powell WS

Maturation of tumor vasculature by interferon-beta disrupts the vascular niche of glioma stem cells

BACKGROUND: The vascular niche necessary for cancer stem cell maintenance is a potential target for cancer therapy. MATERIALS AND METHODS: Human glioma xenografts were treated with IFN-ß delivered systemically via a liver-targeted,...

Authors: Williams RF, Sims TL, Tracey L, Myers AL, Ng CY, Poppleton H, Nathwani AC, Davidoff AM.

Preclinical development of CAT-354, an IL-13 neutralizing antibody, for the treatment of severe uncontrolled asthma

BACKGROUND AND PURPOSE: IL-13 is a pleiotropic Th2 cytokine considered likely to play a pivotal role in asthma. Here we describe the preclinical in vitro and in vivo characterization of CAT-354, an IL-13-neutralizing IgG4 monoclonal antibody (mAb),...

Authors: May RD, Monk PD, Cohen ES, Manuel D, Dempsey F, Davis NH, Dodd AJ, Corkill DJ, Woods J,...

Isoform-specific differences between Rap1A and Rap1B GTPases in the formation of endothelial cell junctions

Rap1 is a Ras-like GTPase that has been studied with respect to its role in cadherin-based cell adhesion. Rap1 exists as two separate isoforms, Rap1A and Rap1B, which are 95% identical and yet the phenotype of the isoform-specific knockout mice is...

Authors: Wittchen ES, Aghajanian A, Burridge K.

Exposure of endothelial cells to physiological levels of myeloperoxidase-modified LDL delays pericellular fibrinolysis.

BACKGROUND: Blood fluidity is maintained by a delicate balance between coagulation and fibrinolysis. The endothelial cell surface is a key player in this equilibrium and cell surface disruptions can upset the balance. We investigated the role of...

Authors: Zouaoui Boudjeltia K, Daher J, Van Antwerpen P, Moguilevsky N, Delree P, Ducobu J, Raes M, Badran B,...

Combination of oncolytic herpes simplex viruses armed with angiostatin and IL-12 enhances antitumor efficacy in human glioblastoma models.

Oncolytic herpes simplex virus (oHSV) can potentially spread throughout the tumor, reach isolated infiltrating cells, kill them, and deliver anticancer agents. However, the host responds to oHSV by inducing intratumoral infiltration of macrophages...

Authors: Zhang W, Fulci G, Wakimoto H, Cheema TA, Buhrman JS, Jeyaretna DS, Stemmer Rachamimov AO, Rabkin SD,...

TNF-a-mediated proliferation of vascular smooth muscle cells involves Raf-1-mediated inactivation of Rb and transcription of E2F1-regulated genes.

Atherosclerosis is characterized by hyperplastic neointima and an inflammatory response with cytokines such as TNFa. TNFa is a pleiotropic cytokine that mediates inflammatory, proliferative, cytostatic and cytotoxic effects in a variety of cell...

Authors: Davis R, Pillai S, Lawrence N, Sebti S, Chellappan SP.

Proteomic identification of protein targets for 15-deoxy-?(12,14)-prostaglandin J2 in neuronal plasma membrane

15-deoxy-?(12,14)-prostaglandin J(2) (15d-PGJ(2)) is one of factors contributed to the neurotoxicity of amyloid ß (Aß), a causative protein of Alzheimer's disease. Type 2 receptor for prostaglandin D(2) (DP2) and peroxysome-proliferator activated...

Authors: Yamamoto Y, Takase K, Kishino J, Fujita M, Okamura N, Sakaeda T, Fujimoto M, Yagami T

First siRNA library screening in hard-to-transfect HUVEC cells.

Meaningful RNAi-based data for target gene identification are strongly dependent on the use of a biologically relevant cell type and efficient delivery of highly functional siRNA reagents into the selected cell type. Here we report the use of the...

Authors: Zumbansen M, Altrogge LM, Spottke NU, Spicker S, Offizier SM, Domzalski SB, St Amand AL, Toell A,...

SST Peer Reviewed

Differential susceptibility of human primary aortic and coronary artery vascular cells to RNA interference.

BACKGROUND: RNAi technology is a promising tool for gene therapy of vascular disease. However, the biological heterogeneity between endothelial (EC) and vascular smooth muscle cells (SMC) and within different vascular beds make them differentially...

Authors: Nabzdyk CS, Chun M, Pradhan Nabzdyk L, Yoshida S, LoGerfo FW

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