Search Results

Molecular mechanisms controlling CFTR gene expression in the airway.

The low levels of CFTR gene expression and paucity of CFTR protein in human airway epithelial cells are not easily reconciled with the pivotal role of the lung in cystic fibrosis pathology. Previous data suggested that the regulatory mechanisms...

Authors: Zhang Z, Ott CJ, Lewandowska MA, Leir SH, Harris A.

Thiazolidinediones induce proliferation of human bronchial epithelial cells through the GPR40 receptor

Thiazolidinediones (TZDs) are synthetic peroxisome proliferator-activated receptor-gamma (PPARgamma) ligands that are widely used in type II diabetes treatment. In addition to their ability to improve glucose homeostasis, TZDs possess...

Authors: Gras D, Chanez P, Urbach V, Vachier I, Godard P, Bonnans C

Nucleosome mapping across the CFTR locus identifies novel regulatory factors

Nucleosome positioning on the chromatin strand plays a critical role in regulating accessibility of DNA to transcription factors and chromatin modifying enzymes. Hence, detailed information on nucleosome depletion or movement at cis-acting regulatory...

Authors: Yigit E, Bischof JM, Zhang Z, Ott CJ, Kerschner JL, Leir SH, Buitrago-Delgado E, Zhang Q, Wang JP,...

Comparison of a high-throughput high-content intracellular Leishmania donovani assay with an axenic amastigote assay.

Visceral leishmaniasis is a neglected tropical disease with significant health impact. The current treatments are poor, and there is an urgent need to develop new drugs. Primary screening assays used for drug discovery campaigns have typically used...

Authors: De Rycker M, Hallyburton I, Thomas J, Campbell L, Wyllie S, Joshi D, Cameron S, Gilbert IH, Wyatt...

SST Peer Reviewed

Tobacco smoke induces polycomb-mediated repression of Dickkopf-1 in lung cancer cells

Limited information is available about epigenetic mechanisms by which cigarette smoke enhances the initiation and progression of lung cancer. To examine this issue, A549 and Calu-6 lung cancer cells were cultured in normal media with or without...

Authors: Hussain M, Rao M, Humphries AE, Hong JA, Liu F, Yang M, Caragacianu D, Schrump DS

Long-lived microRNA-Argonaute complexes in quiescent cells can be activated to regulate mitogenic responses.

Cellular proliferation depends on the integration of mitogenic stimuli with environmental conditions. Increasing evidence suggests that microRNAs play a regulatory role in this integration. Here we show that during periods of cellular quiescence,...

Authors: Olejniczak SH, La Rocca G, Gruber JJ, Thompson CB

SST Peer Reviewed

Nucleosome occupancy reveals regulatory elements of the CFTR promoter.

Access to regulatory elements of the genome can be inhibited by nucleosome core particles arranged along the DNA strand. Hence, sites that are accessible by transcription factors may be located by using nuclease digestion to identify the relative...

Authors: Ott CJ, Bischof JM, Unti KM, Gillen AE, Leir SH, Harris A.

Phospholipid scramblase 1 mediates type i interferon-induced protection against staphylococcal a-toxin

The opportunistic gram-positive pathogen Staphylococcus aureus is a leading cause of pneumonia and sepsis. Staphylococcal a-toxin, a prototypical pore-forming toxin, is a major virulence factor of S. aureus clinical isolates, and lung epithelial...

Authors: Lizak M, Yarovinsky TO

Efficient gene delivery to primary alveolar epithelial cells by nucleofection.

Primary alveolar epithelial cells play a pivotal role in lung research, particularly when focusing on gas exchange, barrier function and transepithelial transport processes. However, efficient transfection of primary alveolar epithelial cells...

Authors: Grzesik BA, Vohwinkel CU, Morty RE, Mayer K, Herold S, Seeger W, Vadász I.

Staphylococcus aureus activates type I IFN signaling in mice and humans through the Xr repeated sequences of protein A

The activation of type I IFN signaling is a major component of host defense against viral infection, but it is not typically associated with immune responses to extracellular bacterial pathogens. Using mouse and human airway epithelial cells, we have...

Authors: Martin FJ, Gomez MI, Wetzel DM, Memmi G, O\'Seaghdha M, Soong G, Schindler C, Prince A

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