Search Results

Easy stable transfection of a human cancer cell line by electrogene transfer with an Epstein-Barr virus-based plasmid vector

We report an easy and stable transfection technique using electrogene transfer with a nonviral Epstein-Barr (EB) virus-based vector. To achieve stable transfection of human breast cancer cells, we conducted electrogene transfer of an EB virus-based...

Authors: Shibata MA, Miwa Y, Morimoto J, Otsuki Y

SST Peer Reviewed

Lymphatic reprogramming of micro vascular endothelial cells by CEA-related Cell Adhesion Molecule-1 via interaction with VEGFR-3 and Prox1

Here, we demonstrate that CEACAM1 is expressed and co-localized with podoplanin in lymphatic endothelial cells (LECs) of tumor but not of normal tissue. CEACAM1 overexpression in HDMECs results in a significant increase of podoplanin positive cells...

Authors: Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, Loges S, Kilic E,...

Prominin-2 is a cholesterol-binding protein associated with apical and basolateral plasmalemmal protrusions in polarized epithelial cells and released into urine

Prominin-2 is a pentaspan membrane glycoprotein structurally related to the cholesterol-binding protein prominin-1, which is expressed in epithelial and non-epithelial cells. Although prominin-1 expression is widespread throughout the organism, the...

Authors: Florek M, Bauer N, Janich P, Wilsch-Braeuninger M, Fargeas CA, Marzesco AM, Ehninger G, Thiele C,...

Cutting Edge: Vitamin D-Mediated Human Antimicrobial Activity against Mycobacterium tuberculosis Is Dependent on the Induction of Cathelicidin

Host defense against intracellular pathogens depends upon innate and adaptive antimicrobial effector pathways. TLR2/1-activation of monocytes leads to the vitamin D-dependent production of cathelicidin and, at the same time, an antimicrobial activity...

Authors: Liu PT, Stenger S, Tang DH, Modlin RL

Target proteins of C/EBPp30 in AML: C/EBPp30 enhances sumoylation of C/EBPp42 via up-regulation of Ubc9

CCAAT/enhancer-binding protein alpha (C/EBPalpha) is a critical regulator for early myeloid differentiation. Mutations in C/EBPalpha occur in 10% of AML patients, leading to the expression of a 30kDa dominant negative isoform (C/EBPalphap30). In the...

Authors: Geletu M, Balkhi MY, Peer Zada AA, Christopeit M, Anto Pulikkan J, Kumar Trivedi A, Tenen DG, Behre...

SST Peer Reviewed

Essential role for Rap1 GTPase and its guanine exchange factor CalDAG-GEFI in LFA-1 but not VLA-4 integrin-mediated human T cell adhesion

Regulated adhesion of T cells by the integrins LFA-1 and VLA-4 is essential for T cell trafficking. The small GTPase Rap1 is a critical activator of both integrins in murine lymphocytes and T cell lines. Here we examined the contribution of the Rap1...

Authors: Ghandour H, Cullere X, Luscinskas FW, Mayadas TN

Commitment to apoptosis in CD4(+) T lymphocytes productively infected with human immunodeficiency virus type 1 is initiated by lysosomal membrane permeabilization, itself induced by the isolated expression of the viral protein Nef

Primary CD4(+) T lymphocytes, supporting in vitro human immunodeficiency virus type 1 (HIV-1) replication, are destined to die by apoptosis. We explored the initial molecular events that act upstream from mitochondrial dysfunction in CD4(+) T...

Authors: Laforge M, Petit F, Estaquier J, Senik A

SST Peer Reviewed

Foxo3 is required for the regulation of oxidative stress in erythropoiesis

Erythroid cells accumulate hemoglobin as they mature and as a result are highly prone to oxidative damage. However, mechanisms of transcriptional control of antioxidant defense in erythroid cells have thus far been poorly characterized. We observed...

Authors: Marinkovic D, Zhang X, Yalcin S, Luciano JP, Brugnara C, Huber T, Ghaffari S

Genetic variants of chemokine receptor CCR7 in patients with systemic lupus erythematosus, Sjogren's syndrome and systemic sclerosis

BACKGROUND: The chemokine receptor CCR7 is a key organizer of the immune system. Gene targeting in mice revealed that Ccr7-deficient animals are severely impaired in the induction of central and peripheral tolerance. Due to these defects,...

Authors: Kahlmann D, Davalos-Misslitz AC, Ohl L, Stanke F, Witte T, Förster R

Clusterin expression can be modulated by changes in TCF1-mediated Wnt signaling

BACKGROUND: Clusterin (CLU) is an enigmatic molecule associated with various physiological processes and disease states. Different modes of cellular stress lead to increased CLU levels, and additionally numerous growth factors and cytokines affect...

Authors: Schepeler T, Mansilla F, Christensen LL, Orntoft TF, Andersen CL

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Easy stable transfection of a human cancer cell line by electrogene transfer with an Epstein-Barr virus-based plasmid vector

Lymphatic reprogramming of micro vascular endothelial cells by CEA-related Cell Adhesion Molecule-1 via interaction with VEGFR-3 and Prox1

Prominin-2 is a cholesterol-binding protein associated with apical and basolateral plasmalemmal protrusions in polarized epithelial cells and released into urine

Cutting Edge: Vitamin D-Mediated Human Antimicrobial Activity against Mycobacterium tuberculosis Is Dependent on the Induction of Cathelicidin

Target proteins of C/EBPp30 in AML: C/EBPp30 enhances sumoylation of C/EBPp42 via up-regulation of Ubc9

Essential role for Rap1 GTPase and its guanine exchange factor CalDAG-GEFI in LFA-1 but not VLA-4 integrin-mediated human T cell adhesion

Commitment to apoptosis in CD4(+) T lymphocytes productively infected with human immunodeficiency virus type 1 is initiated by lysosomal membrane permeabilization, itself induced by the isolated expression of the viral protein Nef

Foxo3 is required for the regulation of oxidative stress in erythropoiesis

Genetic variants of chemokine receptor CCR7 in patients with systemic lupus erythematosus, Sjogren's syndrome and systemic sclerosis

Clusterin expression can be modulated by changes in TCF1-mediated Wnt signaling