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Is the classical Lucetta® Luminometer compatible with MycoAlert® PLUS Assay?

Yes. You can use the MycoAlert® mode of the classical Lucetta® Luminometer AAL-1001 for running MycoAlert® PLUS Assay. However, as the MycoAlert® mode was programmed based on standard MycoAlert® Assay and has not be adapted...

Where are Lonza's normal Adipose Derived Stem Cells isolated from?

Lonza's normal (non-diabetic) Adipose Derived Stem Cells (ADSCs) are isolated from lipoaspirate obtained from liposuction surgeries. As liposuction surgeries typically pool lipoaspirate from multiple locations, a single isolation source is not able...

Do your ProCHO™ Media contain hypoxanthine and thymidine?

All of our ProCHO™ Media, doe not contain Thymidine and Hypoxanthine. So if the customer has a CHO Clone which is dhfr-negativ, he will need to add Thymidine and Hypoxanthine or otherwise the cells will not grow. If the customer has a CHO clone...

What are Silensomes™ HLM?

Silensomes™ HLM are pooled human liver microsomes (HLM) chemically and irreversibly inactivated for one specific cytochrome P450 (CYP450) activity (1A2, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4) using a mechanism based inhibitor (MBI). Silensomes™ HLM...

What is the principle behind the ViaLight™ Plus Cell Proliferation and Cytotoxicity Assay?

ViaLight™ Plus BioAssay Kits use bioluminescence catalysed by the firefly luciferase enzyme to measure intracellular ATP. Since ATP is central to the metabolism of all cells, the intracellular concentration is very precisely...

Do intracellular ATP levels differ between different cell types?

Healthy cells in culture contain maximal amounts of ATP and a wide range of metabolic stimuli do not increase these ATP levels. Any increase in the ATP concentration of a culture can therefore be associated with an increase in the number of...

What can Silensomes™ HLM be used for?

Silensomes™ HLM can be used in cytochrome P450 screening and phenotyping assays, to identify enzymes responsible for the metabolism of drug candidates. Silensomes™ HLM can be used to predict directly the in-vitro fm for a particular CYP450 enzyme (fm...

Can the ViaLight™ Plus Kits be used for both proliferation and cytotoxicity assay

Yes. Since the ATP levels are precisely maintained in healthy cells, the level of ATP accurately reflects the number of viable cells in a culture. In response to a proliferative signal (e.g. a growth factor) the number of cells in a...

What is CYP450 phenotyping?

In vitro identification and measurement of the contribution of the major cytochrome P450 enzymes (CYP450s) involved in the metabolism of a new drug candidate is called “CYP450 phenotyping”. Information from these assays is used to predict the impact...

Does Lonza have a suggested TRAP kit for testing Osteoclast differentiation/functionality?

The TRAP kit Lonza used in the past when testing Osteoclast differentiation/functionality is the Acid Phosphatase, Leukocyte (TRAP) Kit from Sigma-Aldrich (Sigma-Aldrich catalog no. 386A). Now, we are using our OsteoLyse Assay Kit PA-1500 for testing...

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