Data Type


Category

+ Show All

Research Area

+ Show All
523 results sorted by

What factors interfere with the ViaLight Plus Assay?

Certain compounds will quench the light emission in bioluminescent reactions.  Typically these are strong red dyes (however, the ViaLight ™ Plus Assay has been designed to function in all phenol red containing media commonly used in...

Will Lonzas HSMM cells de-differentiate after they have been differentiated to form myotubes?

The differentiation of Lonza’s Human Skeletal Muscle Myoblasts (HSMM) to myotubes is a terminal differentiation and, therefore, once differentiated, these cells will not de-differentiate to myoblasts. Additionally, differentiation is not...

Can Lonzas HSMM or SkMC cells be maintained/subcultured/cryopreserved as myotubes after they have been differentiated?

The differentiation of Lonza’s Human Skeletal Muscle Myoblasts (HSMM) to myotubes is a terminal differentiation and, therefore, once differentiated, these cells will not de-differentiate to myoblasts. Differentiated HSMM myotubes can be maintained in...

Can I store my cryopreserved primary cell ampule at 80°C?

No, cryopreserved cells should be stored in liquid or vapor phase nitrogen. Storing the cells at higher temperatures can irreversibly damage the cells.

How do you calculate a population doubling?

A population doubling is the total number of population doublings of a cell type since its initiation in vitro. The number of population doubling is calculated using the following equations:Cell count at day of passage /Cell count 24 hours after...

Why does Lonza suggest multiple concentrations of Hydrocortisone-21-hemisuccinate and ß-Glycerophosphate when differentiating Normal Human Osteoblasts (NHOst)?

While both hydrocortisone-21-hemisuccinate and ß-glycerophosphate are necessary when differentiating Lonza’s Normal Human Osteoblasts (NHOst), Lonza has noted that some lots of NHOst may be more sensitive to the concentration of...

Why must Lonzas Normal Human Articular Chondrocytes (NHAC-kn) be re-differentiated to express collagen?

Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) are differentiated cells which express collagen when they are initially isolated, however, they lose their differentiated phenotype after serial expansion in monolayer culture so that by the 2nd...

How do you calculate doubling time of a cell culture?

The doubling time is the number of hours it takes for a cell population to double in number. Doubling time is calculated using the following equation:Number of hours from 24 hours after seeding until passage/Population Doublings = Doubling Time...

Do your ProCHO Media contain hypoxanthine and thymidine?

All of our ProCHO™ Media, doe not contain Thymidine and Hypoxanthine. So if the customer has a CHO Clone which is dhfr-negativ, he will need to add Thymidine and Hypoxanthine or otherwise the cells will not grow. If the customer has a CHO clone...

Can serum be used with Lonza's SAECs?

No. Serum will induce squamous differentiation of primary airway epithelial cells. Our SAGM™ Small Airway Epithelial Cell Growth Medium BulletKit CC-3118 does not contain FBS or any other serum.
PAGE 40