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What are your recommendations for minimum and maximum cell numbers for Nucleofection™ ?

The recommended cell number will vary depending on which Optimized protocol is being used. In general, using less than 2x10exp5cells per reaction causes a major increase in cell mortality. For some cell lines we have tried cell numbers up...
Missing: stable linesGuideline

Are there any contaminating glial cells in your Clonetics™ rat or mouse embryonic neuronal cells?

There will be a small amount of glial cells in the culture, these are not considered a contaminating cell type. The glial cells are necessary to help the neurons form their axons and dendrites, synchronize the activity of the axons, and remove...
Missing: stable linesGuideline

Is there a special recommendation on Human T cell enrichment before Nucleofection®?

For Nucleofectio® it is preferable to use Human T cell populations enriched by magnetic separation. For example, MACS™ Microbeads (Miltenyi) or by a rosetting method. For magnetic separation we recommend negative selection or detaching beads...
Missing: stable linesGuideline

Is the age of the mice important for my mouse T cell Nucleofection®?

Yes. We recommend using mice between 6-12 weeks. Using mouse T cells isolated from younger or older animals for Nucleofection® may result in much lower transfection efficiencies and/or viabilities.
Missing: stable linesGuideline

Is the viability of cells from diseased or diabetic donors less than cells from normal donors?

We guarantee > 70 % viability for cells from both diseased/diabetic donors and normal donors.
Missing: stable linesGuideline

Where are Lonza’s Pulmonary Artery Smooth Muscle Cells (PASMC) cells isolated from?

...tissue source to properly isolate a sufficient number of cells. Lonza production estimates that 95% of the time, the pulmonary artery that is used for our PASMCs comes directly off the heart. It is rarely, if ever that we get the branched sections located...
Missing: stable linesGuideline

What is the typical cell distribution for peripheral blood mononuclear cells (PBMC) catalog number CC-2702? 

We don't characterize each lot of these cells. Therefore, we don't make any guarantee regarding cell type distribution.Typically, you would expect to see approximately 60-80% T Cells, 10-20% monocytes, and the remaining cells would...
Missing: stable linesGuideline

What is the difference between Lonza’s ALI screened airway epithelial cells and the non-screened cells?

All of Lonza’s airway epithelial cells receive standard quality control testing in monolayer culture. After cells have passed Lonza’s initial monolayer quality control testing, a few lots receive additional quality control testing to monitor...
Missing: stable linesGuideline

I thawed and plated my ampoule of Clonetics™ Cells yesterday and most of the cells are floating. What happened?

Clonetics™ Cells will not be confluent on day one. After 24 hours, you will see few attached cells per field under the microscope. Aspirate off the media and the floating cells and replace with fresh, pre-warmed media. Those attached cells...
Missing: stable linesGuideline

What kind of protocol you recommend for stimulation of human T cells?

For getting the results we present in our datasheet, we recommend the follwing strategy: Cells should be stimulated and cultivated for 3 days before Nucleofection, preferentially on plates coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibody...
Missing: stable linesGuideline
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