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Hyperactive piggyBac Gene Transfer in Human Cells and In Vivo

We characterized a recently developed hyperactive piggyBac (pB) transposase enzyme [containing seven mutations (7pB)] for gene transfer in human cells in vitro and to somatic cells in mice in vivo. Despite a protein level expression similar to that...

Authors: Joseph E. Doherty,Leslie E. Huye, Kosuke Yusa, Liqin Zhou, Nancy L. Craig, Matthew H. Wilson

High-efficiency transfection and survival rates of embryonic and adult mouse neural stem cells achieved by electroporation

Cells of the central nervous system are notoriously difficult to transfect. This is not only true for neurons and glial cells but also for dividing neural stem and progenitor cells (NSCs). About ten years ago a major advance was provided by...

Authors: Bettina Bertrama, Stefan Wiesea, Alexander von Holst

SST Peer Reviewed

Differences in gene expression and cytokine production by crystalline vs. amorphous silica in human lung epithelial cells.

BACKGROUND: Exposure to respirable crystalline silica particles, as opposed to amorphous silica, is associated with lung inflammation, pulmonary fibrosis (silicosis), and potentially with lung cancer. We used Affymetrix/GeneSifter microarray analysis...

Authors: Perkins TN, Shukla A, Peeters PM, Steinbacher JL, Landry CC, Lathrop SA, Steele C, Reynaert NL,...

FLASH assembly of TALENs for high-throughput genome editing.

Engineered transcription activator–like effector nucleases (TALENs) have shown promise as facile and broadly applicable genome editing tools. However, no publicly available high-throughput method for constructing TALENs has been published, and...

Authors: Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK.

SST Peer Reviewed

TGF-beta1 induced epithelial to mesenchymal transition (EMT) in human bronchial epithelial cells is enhanced by IL-1beta but not abrogated by corticosteroids

BACKGROUND: Chronic persistent asthma is characterized by ongoing airway inflammation and airway remodeling. The processes leading to airway remodeling are poorly understood, and there is increasing evidence that even aggressive anti-inflammatory...

Authors: Doerner AM, Zuraw BL

Loss of TRPC1-mediated Ca2+ influx contributes to impaired degranulation in Fyn-deficient mouse bone marrow-derived mast cells.

MC degranulation requires the influx of calcium from the extracellular environment. Orai1/STIM1 is essential to MC SOCE, as shown in rat peritoneal MCs, the rat MC lines (RBL-2H3), or in Orai1 null embryo liver-derived, cultured MCs. However, minimal...

Authors: Suzuki R, Liu X, Olivera A, Aguiniga L, Yamashita Y, Blank U, Ambudkar I, Rivera J.

SST Peer Reviewed

Biological roles of nontypeable Haemophilus influenzae type IV pilus proteins encoded by the pil and com operons

We previously demonstrated that one or more products of the genes in the pil and com gene clusters of the opportunistic human respiratory pathogen nontypeable Haemophilus influenzae (NTHI) are required for type IV pilus (Tfp) biogenesis and function....

Authors: Carruthers MD, Tracy EN, Dickson AC, Ganser KB, Munson RS Jr, Bakaletz LO

CD56 expression in human myeloma cells derived from the neurogenic gene expression: possible role of the SRY-HMG box gene, SOX4.

CD56 is frequently detected on primary myeloma cells from more than 80% patients with overt myeloma. In order to clarify the possible mechanisms of CD56 expression in human myeloma, we underwent screening for potential targets by microarray analysis,...

Authors: Iqbal MS, Otsuyama K, Shamsasenjan K, Asaoku H, Kawano MM.

The lysophosphatidic acid receptor LPA1 promotes epithelial cell apoptosis after lung injury

Increased epithelial cell apoptosis in response to lung injury has been implicated in the development of idiopathic pulmonary fibrosis (IPF), but the molecular pathways promoting epithelial cell apoptosis in this disease have yet to be fully...

Authors: Funke M, Zhao Z, Xu Y, Chun J, Tager AM

Examination of transcriptional networks reveals an important role for TCFAP2C, SMARCA4, and EOMES in trophoblast stem cell maintenance

Trophoblast stem cells (TS cells), derived from the trophectoderm (TE) of blastocysts, require transcription factors (TFs) and external signals (FGF4, INHBA/NODAL/TGFB1) for self-renewal. While many reports have focused on TF networks that regulate...

Authors: Kidder, BL and Palmer S

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Hyperactive piggyBac Gene Transfer in Human Cells and In Vivo

High-efficiency transfection and survival rates of embryonic and adult mouse neural stem cells achieved by electroporation

Differences in gene expression and cytokine production by crystalline vs. amorphous silica in human lung epithelial cells.

FLASH assembly of TALENs for high-throughput genome editing.

TGF-beta1 induced epithelial to mesenchymal transition (EMT) in human bronchial epithelial cells is enhanced by IL-1beta but not abrogated by corticosteroids

Loss of TRPC1-mediated Ca2+ influx contributes to impaired degranulation in Fyn-deficient mouse bone marrow-derived mast cells.

Biological roles of nontypeable Haemophilus influenzae type IV pilus proteins encoded by the pil and com operons

CD56 expression in human myeloma cells derived from the neurogenic gene expression: possible role of the SRY-HMG box gene, SOX4.

The lysophosphatidic acid receptor LPA1 promotes epithelial cell apoptosis after lung injury

Examination of transcriptional networks reveals an important role for TCFAP2C, SMARCA4, and EOMES in trophoblast stem cell maintenance