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Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi.

BACKGROUND: Trypanosoma cruzi, a kinetoplastid protozoan parasite that causes Chagas disease, infects approximately 15 million people in Central and South America. In contrast to the substantial in silico studies of the T. cruzi genome,...

Authors: Xu D, BrandÜ¡n CP, BasombrÜo MA, Tarleton RL.

SST Peer Reviewed

A novel phosphatase cascade regulates differentiation in Trypanosoma brucei via a glycosomal signaling pathway.

In the mammalian bloodstream, the sleeping sickness parasite Trypanosoma brucei is held poised for transmission by the activity of a tyrosine phosphatase, TbPTP1. This prevents differentiation of the transmissible "stumpy forms" until entry into the...

Authors: Szöor B, Ruberto I, Burchmore R, Matthews KR.

Characterization of extended co-culture of non-typeable Haemophilus influenzae with primary human respiratory tissues

Non-typeable Haemophilus influenzae (NTHi) are human-adapted Gram-negative bacteria that comprise part of the normal flora of the human upper airway, but are also responsible for a number of mucosal infections such as otitis media and bronchitis....

Authors: Ren D, Nelson KL, Uchakin PN, Smith AL, Gu XX, Daines DA.

Site-specific DNA double-strand breaks greatly increase stable transformation efficiency in Trypanosoma brucei.

Genetic manipulation in African trypanosomes typically relies upon electroporation with chromosomal integration of DNA constructs by homologous recombination. Relatively little is known about chromosomal recombination and repair in these organisms...

Authors: Glover L, Horn D.

SST Peer Reviewed

Reassortment between Avian H5N1 and human influenza viruses is mainly restricted to the matrix and neuraminidase gene segments

Highly pathogenic avian influenza H5N1 viruses have devastated the poultry industry in many countries of the eastern hemisphere. Occasionally H5N1 viruses cross the species barrier and infect humans, sometimes with a severe clinical outcome. When...

Authors: Schrauwen EJ, Bestebroer TM, Rimmelzwaan GF, Osterhaus AD, Fouchier RA, Herfst S.

Comparative transfection of DNA into primary and transformed mammalian cells from different lineages

BACKGROUND: The delivery of DNA into human cells has been the basis of advances in the understanding of gene function and the development of genetic therapies. Numerous chemical and physical approaches have been used to deliver the DNA, but their...

Authors: Maurisse R, De Semir D, Emamekhoo H, Bedayat B, Abdolmohammadi A, Parsi H, Gruenert DC

SST Peer Reviewed

Evaluation of differentiated human bronchial epithelial cell culture systems for asthma research.

The aim of the current study was to evaluate primary (human bronchial epithelial cells, HBEC) and non-primary (Calu-3, BEAS-2B, BEAS-2B R1) bronchial epithelial cell culture systems as air-liquid interface- (ALI-) differentiated models for asthma...

Authors: Stewart CE, Torr EE, Mohd Jamili NH, Bosquillon C, Sayers I.

Molecular analysis of chondrocytes cultured in agarose in response to dynamic compression.

BACKGROUND: Articular cartilage is exposed to high mechanical loads under normal physiological conditions and articular chondrocytes regulate the composition of cartilaginous matrix, in response to mechanical signals. However, the intracellular...

Authors: Bougault C, Paumier A, Aubert-Foucher E, Mallein-Gerin F.

Kinase/phosphatase overexpression reveals pathways regulating hippocampal neuron morphology

Development and regeneration of the nervous system requires the precise formation of axons and dendrites. Kinases and phosphatases are pervasive regulators of cellular function and have been implicated in controlling axodendritic development and...

Authors: Buchser WJ, Slepak TI, Gutierrez-Arenas O, Bixby JL, Lemmon VP

SST Peer Reviewed

Cadmium increases HIF-1 and VEGF expression through ROS, ERK, and AKT signaling pathways and induces malignant transformation of human bronchial epithelial cells.

Cadmium is categorized as a human carcinogen especially involved in lung cancers. Angiogenesis is considered a fundamental requirement for tumorigenesis, but the mechanisms underlying the tumor angiogenesis induced by cadmium are poorly understood....

Authors: Jing Y, Liu LZ, Jiang Y, Zhu Y, Guo NL, Barnett J, Rojanasakul Y, Agani F, Jiang BH.

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Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi.

A novel phosphatase cascade regulates differentiation in Trypanosoma brucei via a glycosomal signaling pathway.

Characterization of extended co-culture of non-typeable Haemophilus influenzae with primary human respiratory tissues

Site-specific DNA double-strand breaks greatly increase stable transformation efficiency in Trypanosoma brucei.

Reassortment between Avian H5N1 and human influenza viruses is mainly restricted to the matrix and neuraminidase gene segments

Comparative transfection of DNA into primary and transformed mammalian cells from different lineages

Evaluation of differentiated human bronchial epithelial cell culture systems for asthma research.

Molecular analysis of chondrocytes cultured in agarose in response to dynamic compression.

Kinase/phosphatase overexpression reveals pathways regulating hippocampal neuron morphology

Cadmium increases HIF-1 and VEGF expression through ROS, ERK, and AKT signaling pathways and induces malignant transformation of human bronchial epithelial cells.