Search Results

Validation of a NAT-based Mycoplasma assay according European Pharmacopoiea.

Eucaryotic expression systems are widely used to produce biologicals for human use, e.g. vaccines, recombinant proteins and monoclonal antibodies. As part of the safety testing the current U.S. Food and Drug Administration (FDA) regulatory guidelines...

Authors: Deutschmann SM, Kavermann H, Knack Y.

SST Peer Reviewed

Identification of gene signatures and molecular markers for human lung cancer prognosis using an in vitro lung carcinogenesis system

Lung cancer continues to be a major deadly malignancy. The mortality of this disease could be reduced by improving the ability to predict cancer patients' survival. We hypothesized that genes differentially expressed among cells constituting an in...

Authors: Kadara H, Lacroix L, Behrens C, Solis L, Gu X, Lee JJ, Tahara E, Lotan D, Hong WK, Wistuba II, Lotan...

Immune depletion with cellular mobilization imparts immunoregulation and reverses autoimmune diabetes in nonobese diabetic mice

OBJECTIVE: The autoimmune destruction of beta-cells in type 1 diabetes results in a loss of insulin production and glucose homeostasis. As such, an immense interest exists for the development of therapies capable of attenuating this destructive...

Authors: Parker MJ, Xue S, Alexander JJ, Wasserfall CH, Campbell-Thompson ML, Battaglia M, Gregori S, Mathews...

Evaluation of three commercial kits for mycoplasma NAT assays: selection and quality improvement.

Authors: Dorange F, Le Goff F, Dumey N

SST Peer Reviewed

Cross comparison of rapid mycoplasma detection platforms

The use of animal and plant derived raw materials in mammalian cell culture processes may provide a possible route of entry for adventitious contaminants such as mycoplasma. Mycoplasma contaminations of cell culture represent a serious challenge to...

Authors: Lawrence B, Bashiri H, Dehghani H

SST Peer Reviewed

Human induced pluripotent stem cells free of vector and transgene sequences.

Reprogramming differentiated human cells to induced pluripotent stem (iPS) cells has applications in basic biology, drug development, and transplantation. Human iPS cell derivation previously required vectors that integrate into the genome, which can...

Authors: Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA.

SST Peer Reviewed

Cigarette smoke extract reduces VEGF in primary human airway epithelial cells

Reduced vascular endothelial growth factor (VEGF) has been reported in bronchoalveolar lavage fluid and lungs of severe emphysema patients. Airway epithelial cells (AEC) are exposed to various environmental insults like cigarette smoke and bacterial...

Authors: Thaikoottathil JV, Martin RJ, Zdunek J, Weinberger A, Rino JG, Chu HW

Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector.

Induced pluripotent stem (iPS) cells have generated keen interest due to their potential use in regenerative medicine. They have been obtained from various cell types of both mice and humans by exogenous delivery of different combinations of Oct4,...

Authors: Gonzalez F, Barragan Monasterio M, Tiscornia G, Montserrat Pulido N, Vassena R, Batlle Morera L,...

Lung epithelial injury by B. anthracis lethal toxin is caused by MKK-dependent loss of cytoskeletal integrity

Bacillus anthracis lethal toxin (LT) is a key virulence factor of anthrax and contributes significantly to the in vivo pathology. The enzymatically active component is a Zn(2+)-dependent metalloprotease that cleaves most isoforms of mitogen-activated...

Authors: Lehmann M, Noack D, Wood M, Perego M, Knaus UG.

The Anti-Inflammatory Effects of Adiponectin Are Mediated via a Heme Oxygenase-1–Dependent Pathway in Rat Kupffer Cells

Altered expression and activity of immunomodulatory cytokines plays a major role in the pathogenesis of alcoholic liver disease. Chronic ethanol feeding increases the sensitivity of Kupffer cells, the resident hepatic macrophage, to...

Authors: Palash Mandal, Pil-Hoon Park, Megan R. McMullen, Brian T. Pratt, and Laura E. Nagy

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Validation of a NAT-based Mycoplasma assay according European Pharmacopoiea.

Identification of gene signatures and molecular markers for human lung cancer prognosis using an in vitro lung carcinogenesis system

Immune depletion with cellular mobilization imparts immunoregulation and reverses autoimmune diabetes in nonobese diabetic mice

Evaluation of three commercial kits for mycoplasma NAT assays: selection and quality improvement.

Cross comparison of rapid mycoplasma detection platforms

Human induced pluripotent stem cells free of vector and transgene sequences.

Cigarette smoke extract reduces VEGF in primary human airway epithelial cells

Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector.

Lung epithelial injury by B. anthracis lethal toxin is caused by MKK-dependent loss of cytoskeletal integrity

The Anti-Inflammatory Effects of Adiponectin Are Mediated via a Heme Oxygenase-1–Dependent Pathway in Rat Kupffer Cells