Search Results

Modulation of Kv3.4 channel N-type inactivation by protein kinase C shapes the action potential in dorsal root ganglion neurons.

Fast inactivation of heterologously expressed Kv3.4 channels is dramatically slowed upon phosphorylation of the channel's N-terminal (N-type) inactivation gate by protein kinase C (PKC). However, the presence and physiological importance of this...

Authors: Ritter DM, Ho C, O'Leary ME, Covarrubias M.

SST Peer Reviewed

Virus-free induction of pluripotency and subsequent excision of reprogramming factors

Reprogramming of somatic cells to pluripotency, thereby creating induced pluripotent stem (iPS) cells, promises to transform regenerative medicine. Most instances of direct reprogramming have been achieved by forced expression of defined factors...

Authors: Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, Woltjen K

SST Peer Reviewed

The oncoprotein SF2/ASF promotes non-small cell lung cancer survival by enhancing survivin expression.

PURPOSE: SF2/ASF is a splicing factor recently described as an oncoprotein. In the present work, we examined the role of SF2/ASF in human non-small cell lung cancer (NSCLC) and analyzed the molecular mechanisms involved in SF2/ASF-related...

Authors: Ezponda T, Pajares MJ, Agorreta J, Echeveste JI, López-Picazo JM, Torre W, Pio R, Montuenga LM.

Identification of novel deregulated RNA metabolism-related genes in non-small cell lung cancer

Lung cancer is a leading cause of cancer death worldwide. Several alterations in RNA metabolism have been found in lung cancer cells; this suggests that RNA metabolism-related molecules are involved in the development of this pathology. In this...

Authors: Valles I, Pajares MJ, Segura V, Guruceaga E, Gomez-Roman J, Blanco D, Tamura A, Montuenga LM, Pio...

Methods for siRNA-mediated Reduction of mRNA and Protein Expression in Human Placental Explants, Isolated Primary Cells and Cell Lines

The use of RNA interference (RNAi) to deplete individual proteins from cells or tissue has revolutionised our ability to characterise gene function. The placenta is an attractive target for studies in which the role of specific proteins can be...

Authors: K. Forbes, M. Desforges, R. Garside, J.D. Aplin, and M. Westwood

Heterodimerization controls localization of Duox-DuoxA NADPH oxidases in airway cells.

Duox NADPH oxidases generate hydrogen peroxide at the air-liquid interface of the respiratory tract and at apical membranes of thyroid follicular cells. Inactivating mutations of Duox2 have been linked to congenital hypothyroidism, and epigenetic...

Authors: Luxen S, Noack D, Frausto M, Davanture S, Torbett BE, Knaus UG

Glucocorticoids sensitize the innate immune system through regulation of the NLRP3 inflammasome.

Glucocorticoids have long been recognized as powerful anti-inflammatory compounds that are one of the most widely prescribed classes of drugs in the world. However, their role in the regulation of innate immunity is not well understood. We sought to...

Authors: Busillo JM, Azzam KM, Cidlowski JA.

Severe impairment of IFN-? and IFN-? responses in cells of a patient with a novel STAT1 splicing mutation.

Subjects affected by Signal Transducer and Activator of Transcription 1 (STAT1) deficiency have lethal bacterial and viral infections. Complete STAT1 deficiency is inherited as an autosomal recessive disease; partial STAT1 deficiency is inherited as...

Authors: Vairo D, Tassone L, Tabellini G, Tamassia N, Gasperini S, Bazzoni F, Plebani A, Porta F, Notarangelo...

Integrative discovery of epigenetically derepressed cancer testis antigens in NSCLC

BACKGROUND: Cancer/testis antigens (CTAs) were first discovered as immunogenic targets normally expressed in germline cells, but differentially expressed in a variety of human cancers. In this study, we used an integrative epigenetic screening...

Authors: Glazer CA, Smith IM, Ochs MF, Begum S, Westra W, Chang SS, Sun W, Bhan S, Khan Z, Ahrendt S,...

Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection

Background: Nucleofection is an emerging technology for delivery of nucleic acids into both the cytoplasm and nucleus of eukaryotic cells with high efficiency. This makes it an ideal technology for gene delivery and siRNA applications. A 96-well...

Authors: Bradburne C, Robertson K, Thach D

SST Peer Reviewed

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Modulation of Kv3.4 channel N-type inactivation by protein kinase C shapes the action potential in dorsal root ganglion neurons.

Virus-free induction of pluripotency and subsequent excision of reprogramming factors

The oncoprotein SF2/ASF promotes non-small cell lung cancer survival by enhancing survivin expression.

Identification of novel deregulated RNA metabolism-related genes in non-small cell lung cancer

Methods for siRNA-mediated Reduction of mRNA and Protein Expression in Human Placental Explants, Isolated Primary Cells and Cell Lines

Heterodimerization controls localization of Duox-DuoxA NADPH oxidases in airway cells.

Glucocorticoids sensitize the innate immune system through regulation of the NLRP3 inflammasome.

Severe impairment of IFN-? and IFN-? responses in cells of a patient with a novel STAT1 splicing mutation.

Integrative discovery of epigenetically derepressed cancer testis antigens in NSCLC

Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection