Search Results

Transcriptome comparison of human neurons generated using induced pluripotent stem cells derived from dental pulp and skin fibroblasts.

Induced pluripotent stem cell (iPSC) technology is providing an opportunity to study neuropsychiatric disorders through the capacity to grow patient-specific neurons in vitro. Skin fibroblasts obtained by biopsy have been the most reliable source of...

Authors: Chen J, Lin M, Foxe JJ, Pedrosa E, Hrabovsky A, Carroll R, Zheng D, Lachman HM.

SST Peer Reviewed

Stable gene transfer and expression in cord blood-derived CD34+ hematopoietic stem and progenitor cells by a hyperactive Sleeping Beauty transposon system.

Here we report stable gene transfer in cord blood-derived CD34(+) hematopoietic stem cells using a hyperactive nonviral Sleeping Beauty (SB) transposase (SB100X). In colony-forming assays, SB100X mediated the highest efficiency (24%) of stable...

Authors: Xue X, Huang X, Nodland SE, Mátés L, Ma L, Izsvák Z, Ivics Z, LeBien TW, McIvor RS, Wagner JE, Zhou...

SST Peer Reviewed

A matrix micropatterning platform for cell localization and stem cell fate determination.

To study the role of cell-extracellular matrix (ECM) interactions, microscale approaches provide the potential to perform high throughput assessment of the effect of the ECM microenvironment on cellular function and phenotype. Using a microscale...

Authors: Huang NF, Patlolla B, Abilez O, Sharma H, Rajadas J, Beygui RE, Zarins CK, Cooke JP

Preparation of reference strains for validation and comparison of mycoplasma testing methods

AIMS: To optimize growth conditions for preparation of stocks of mycoplasma reference strains to obtain highly viable and disperse samples with low ratios of genomic copy (GC) number to that of colony forming units (CFU). These stocks are required...

Authors: Dabrazhynetskaya A, Volokhov DV, David SW, Ikonomi P, Brewer A, Chang A, Chizhikov V.

SST Peer Reviewed

Cardiotoxicity of the anticancer therapeutic agent bortezomib

Recent case reports provided alarming signals that treatment with bortezomib might be associated with cardiac events. In all reported cases, patients experiencing cardiac problems were previously or concomitantly treated with other chemotherapeutics...

Authors: Nowis D, Maczewski M, Mackiewicz U, Kujawa M, Ratajska A, Wieckowski MR, Wilczynski GM, Malinowska...

Optimization of adult sensory neuron electroporation to study mechanisms of neurite growth

The development of eukaryotic transfection technologies has been rapid in recent years, providing the opportunity to better analyze cell-autonomous mechanisms influencing various cellular processes, including cell-intrinsic regulators of regenerative...

Authors: McCall J, Nicholson L, Weidner N, Blesch A.

SST Peer Reviewed

Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-a.

Platelet-derived growth factor receptor a (PDGFR-a) and stem cell antigen 1 (Sca-1) have recently been identified as selective markers of mouse mesenchymal stem cells (MSCs). PDGFR-a(+)Sca-1(+) (PaS) MSCs have augmented growth potential and robust...

Authors: Houlihan DD, Mabuchi Y, Morikawa S, Niibe K, Araki D, Suzuki S, Okano H, Matsuzaki Y.

SST Peer Reviewed

Epithelial-derived TGF-beta2 modulates basal and wound-healing subepithelial matrix homeostasis.

The epithelium influences the mesenchyme during dynamic processes such as embryogenesis, wound healing, fibrosis, and carcinogenesis. Since transforming growth factor-beta (TGF-beta) modulates these processes, we hypothesized that epithelial-derived...

Authors: Thompson HG, Mih JD, Krasieva TB, Tromberg BJ, George SC.

Vesicular trafficking through cortical actin during exocytosis is regulated by the Rab27a effector JFC1/Slp1 and the RhoA-GAP GMIP

Cytoskeleton remodeling is important for the regulation of vesicular transport associated with exocytosis but a direct association between granular secretory proteins and actin remodeling molecules has not been shown and this mechanism remains...

Authors: Johnson JL, Monfregola J, Napolitano G, Kiosses WB, Catz SD

SST Peer Reviewed

Contributions of TRPV1, endovanilloids, and endoplasmic reticulum stress in lung cell death in vitro and lung injury.

Endogenous agonists of transient receptor potential vanilloid-1 (TRPV1) (endovanilloids) are implicated as mediators of lung injury during inflammation. This study tested the hypothesis that endovanilloids produced following lipopolysaccharide (LPS)...

Authors: Thomas KC, Roberts JK, Deering-Rice CE, Romero EG, Dull RO, Lee J, Yost GS, Reilly CA.

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Transcriptome comparison of human neurons generated using induced pluripotent stem cells derived from dental pulp and skin fibroblasts.

Stable gene transfer and expression in cord blood-derived CD34+ hematopoietic stem and progenitor cells by a hyperactive Sleeping Beauty transposon system.

A matrix micropatterning platform for cell localization and stem cell fate determination.

Preparation of reference strains for validation and comparison of mycoplasma testing methods

Cardiotoxicity of the anticancer therapeutic agent bortezomib

Optimization of adult sensory neuron electroporation to study mechanisms of neurite growth

Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-a.

Epithelial-derived TGF-beta2 modulates basal and wound-healing subepithelial matrix homeostasis.

Vesicular trafficking through cortical actin during exocytosis is regulated by the Rab27a effector JFC1/Slp1 and the RhoA-GAP GMIP

Contributions of TRPV1, endovanilloids, and endoplasmic reticulum stress in lung cell death in vitro and lung injury.