Search Results

Do you have a Nucleofection® Protocol for transfection of yeast (Saccharomyces cerevisiae)?

Unfortunately we do not have any records about Nucleofection® of yeast. It is also impossible to search for current publications, because you get only false positive hits. A lot of yeast genes have been nucleofected and many reportergene systems have...

Can Lonza’s HSMM or SkMC cells be maintained/subcultured/cryopreserved as myotubes after they have been differentiated?

The differentiation of Lonza’s Human Skeletal Muscle Myoblasts (HSMM) to myotubes is a terminal differentiation and, therefore, once differentiated, these cells will not de-differentiate to myoblasts. Differentiated HSMM myotubes can be maintained in...

Why does Lonza suggest multiple concentrations of Hydrocortisone-21-hemisuccinate and ß-Glycerophosphate when differentiating Normal Human Osteoblasts (NHOst)?

While both hydrocortisone-21-hemisuccinate and ß-glycerophosphate are necessary when differentiating Lonza’s Normal Human Osteoblasts (NHOst), Lonza has noted that some lots of NHOst may be more sensitive to the concentration of...

Why must Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) be re-differentiated to express collagen?

Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) are differentiated cells which express collagen when they are initially isolated, however, they lose their differentiated phenotype after serial expansion in monolayer culture so that by the 2nd...

Will Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) express collagen Type X? Are Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) hypertrophic following re-differentiation?

Lonza guarantees that the Normal Human Articular Chondrocytes (NHAC-kn) will express collagen Type II and sulfated proteoglycans (can be stained with Alcian Blue) upon re-differentiation. Lonza’s recommended differentiation protocol does induce...

How can I submit references to Lonza for inclusion in the Lonza Citation Database?

Lonza welcomes submissions of published scholarly article from researchers detailing the use of Lonza products. Please email reference submissions to Scientific.Support.eu@lonza.com. Please indicate if you would like to make the reference publically...

Where can I obtain the Safety Data Sheet (SDS) for a Lonza product?

Safety Data Sheet (SDS) [also called Material Safety Data Sheet (MSDS)] for all Lonza research products can be obtained by going to the product page.From any page on the Lonza Bioscience website (https://bioscience.lonza.com/), search for the product...

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