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514 results sorted by
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T
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Dried agarose on the comb teeth is a frequent cause of this problem. Prior to casting your gel, check the comb teeth for residual dried agarose.If not removed, this will attach to the newly cast agarose and fracture the well upon comb removal. This...
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We do not recommend reusing the conductive polymer cuvettes as performance decreases when cuvettes are used more than once.
D
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Preparation of coverslips: At Lonza we used coverslips from Menzel (part of Thermo Scientific). We think that the coverslips have such a good quality that they don’t have to be cleaned prior to use.If you want to use other coverslips or prefer...
H
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The amount to load per well is variable. What is most important is how much DNA there is in the bands you wish to resolve. The least amount of DNA that can be consistently detected with ethidium bromide is about 10 ng. The most DNA you can have in a...
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1
6
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N
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t
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®
S
t
r
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?
This depends on the cell type you are working with. In our Optimized Protocols the cell numbers range from 2.5 x 10^4 cells (mouse DC) up to 1x 10^6 cells (human T cells). Detailed information is provided in our 4D-Nucleofector® Optimized Protocols.
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We recommend running agarose gels at 4–10 volts/cm (cm is determined by measuring the interelectrode distance, not the gel length) under normal horizontal electrophoretic conditions.If the voltage is too high, band streaking, especially for DNA...
D
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1
6
-
w
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l
N
u
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l
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o
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u
v
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t
t
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®
?
No, each well can be addressed separately. You may apply up to 16 different programs to one 16-well Nucleocuvette® Strip.
W
h
a
t
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s
t
h
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d
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f
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w
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S
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e
v
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™
3
:
1
a
n
d
N
u
S
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v
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™
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T
G
™
A
g
a
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o
s
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s
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NuSieve™ 3:1 Agarose is a standard melting temperature agarose. The resolution range for NuSieve™ 3:1 Agarose is 50 bp–1000 bp. NuSieve™ 3:1 Agarose is designed for analytical electrophoresis; its high gel strength also makes it ideal for use in...
C
a
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y
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t
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A
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R
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™
A
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a
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p
r
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a
d
i
p
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c
y
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s
?
Yes, the AdipoRed™ Assay Reagent can be used on many other cell types that accumulate triglycerides. It may have to be optimized for specific applications depending on the lipid that is stained.
H
o
w
m
a
n
y
1
0
0
µ
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N
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c
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4
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®
S
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m
?
The 4D-Nucleofector® X Unit can work with two 100µl Nucleocuvettes® in parallel. However, the operation software allows defining up to 50 Nucleocuvettes® per experiment. These cuvettes will be transfected by the system continuously in...
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