Search Results

Changes in expression of drug-metabolizing enzymes by single-walled carbon nanotubes in human respiratory tract cells.

Single-walled carbon nanotubes (SWCNTs) have attracted attention for biomedical and biotechnological applications, such as drug delivery. However, there are concerns about the safety of SWCNTs for use in humans. To investigate the potential use of...

Authors: Hitoshi K, Katoh M, Suzuki T, Ando Y, Nadai M.

Nucleofection of whole murine retinas.

The mouse retina constitutes an important research model for studies aiming to unravel the cellular and molecular mechanisms underlying ocular diseases. The accessibility of this tissue and its feasibility to directly obtain neurons from it has...

Authors: Gomez-Touriño IM, Senra A, Garcia F.

SST Peer Reviewed

Up-regulation of sonic hedgehog contributes to TGF-ß1-induced epithelial to mesenchymal transition in NSCLC cells

BACKGROUND: Lung cancer, especially non-small cell lung cancer (NSCLC) is the major cause of cancer-related deaths in the United States. The aggressiveness of NSCLC has been shown to be associated with the acquisition of epithelial-to-mesenchymal...

Authors: Maitah MY, Ali S, Ahmad A, Gadgeel S, Sarkar FH.

Methods for siRNA-mediated reduction of mRNA and protein expression in human placental explants, isolated primary cells and cell lines.

The use of RNA interference (RNAi) to deplete individual proteins from cells or tissue has revolutionised our ability to characterise gene function. The placenta is an attractive target for studies in which the role of specific proteins can be...

Authors: Forbes K, Desforges M, Garside R, Aplin JD, Westwood M.

Klebsiella pneumoniae outer membrane protein A is required to prevent the activation of airway epithelial cells

Outer membrane protein A (OmpA) is a class of proteins highly conserved among the Enterobacteriaceae family and throughout evolution. Klebsiella pneumoniae is a capsulated gram-negative pathogen. It is an important cause of community-acquired and...

Authors: March C, Moranta D, Regueiro V, Llobet E, Tomás A, Garmendia J, Bengoechea JA.

Peptide-mediated disruption of calmodulin-cyclin E interactions inhibits proliferation of vascular smooth muscle cells and neointima formation.

RATIONALE: Cell cycle progression in vascular smooth muscle cells (VSMCs) is a therapeutic target for restenosis. OBJECTIVE: Having discovered that calmodulin (CaM)-dependent cyclin E/CDK2 activity underlies Ca(2+)-sensitive G(1)-to-S phase...

Authors: Hui S, Choi J, Zaidi S, Momen A, Steinbach SK, Sadi AM, Ban K, Husain M.

Alternaria-induced release of IL-18 from damaged airway epithelial cells: an NF-?B dependent mechanism of Th2 differentiation?

BACKGROUND: A series of epidemiologic studies have identified the fungus Alternaria as a major risk factor for asthma. The airway epithelium plays a critical role in the pathogenesis of allergic asthma. These reports suggest that activated airway...

Authors: Murai H, Qi H, Choudhury B, Wild J, Dharajiya N, Vaidya S, Kalita A, Bacsi A, Corry D, Kurosky A,...

Silica induces NLRP3 inflammasome activation in human lung epithelial cells.

BACKGROUND: In myeloid cells the inflammasome plays a crucial role in innate immune defenses against pathogen- and danger-associated patterns such as crystalline silica. Respirable mineral particles impinge upon the lung epithelium causing...

Authors: Peeters PM, Perkins TN, Wouters EF, Mossman BT, Reynaert NL.

In vitro reprogramming of adult hepatocytes into insulin-producing cells without viral vectors.

The pancreas and the liver share the same endodermal origin. We have been studying whether mature hepatocytes can be induced to differentiate into pancreatic beta-cells by in vitro delivery of transcriptional factors using a non-viral approach. Here...

Authors: Motoyama H, Ogawa S, Kubo A, Miwa S, Nakayama J, Tagawa Y, Miyagawa S.

Precision genome engineering with programmable DNA-nicking enzymes

Zinc finger nucleases (ZFNs) are powerful tools of genome engineering but are limited by their inevitable reliance on error-prone nonhomologous end-joining (NHEJ) repair of DNA double-strand breaks (DSBs), which gives rise to randomly generated,...

Authors: Kim E, Kim S, Kim DH, Choi BS, Choi IY, Kim JS

Data Type

Category

Research Area

Platform

Topic

Species

Characteristic

Format

Origin