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Hepcidin expression in human airway epithelial cells is regulated by interferon-gamma.

BACKGROUND: Hepcidin serves as a major regulator of systemic iron metabolism and immune function. Airway epithelial cells have an extensive interface with the environment, and so must be able to respond locally to the presence of particulates,...

Authors: Frazier MD, Mamo LB, Ghio AJ, Turi JL.

Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures.

To identify accessible and permissive human cell types for efficient derivation of induced pluripotent stem cells (iPSCs), we investigated epigenetic and gene expression signatures of multiple postnatal cell types such as fibroblasts and blood cells....

Authors: Chou BK, Mali P, Huang X, Ye Z, Dowey SN, Resar LM, Zou C, Zhang YA, Tong J, Cheng L.

SST Peer Reviewed

MARCKS and related chaperones bind to unconventional myosin V isoforms in airway epithelial cells

We have shown previously that myristoylated alanine-rich C kinase substrate (MARCKS) is a key regulatory molecule in the process of mucin secretion by airway epithelial cells, and that part of the secretory mechanism involves intracellular...

Authors: Lin KW, Fang S, Park J, Crews AL, Adler KB.

Thiazolidinediones induce proliferation of human bronchial epithelial cells through the GPR40 receptor

Thiazolidinediones (TZDs) are synthetic peroxisome proliferator-activated receptor-gamma (PPARgamma) ligands that are widely used in type II diabetes treatment. In addition to their ability to improve glucose homeostasis, TZDs possess...

Authors: Gras D, Chanez P, Urbach V, Vachier I, Godard P, Bonnans C

Molecular analysis of chondrocytes cultured in agarose in response to dynamic compression.

BACKGROUND: Articular cartilage is exposed to high mechanical loads under normal physiological conditions and articular chondrocytes regulate the composition of cartilaginous matrix, in response to mechanical signals. However, the intracellular...

Authors: Bougault C, Paumier A, Aubert-Foucher E, Mallein-Gerin F.

Tobacco smoke induces polycomb-mediated repression of Dickkopf-1 in lung cancer cells

Limited information is available about epigenetic mechanisms by which cigarette smoke enhances the initiation and progression of lung cancer. To examine this issue, A549 and Calu-6 lung cancer cells were cultured in normal media with or without...

Authors: Hussain M, Rao M, Humphries AE, Hong JA, Liu F, Yang M, Caragacianu D, Schrump DS

Hyperactive piggyBac Gene Transfer in Human Cells and In Vivo

We characterized a recently developed hyperactive piggyBac (pB) transposase enzyme [containing seven mutations (7pB)] for gene transfer in human cells in vitro and to somatic cells in mice in vivo. Despite a protein level expression similar to that...

Authors: Joseph E. Doherty,Leslie E. Huye, Kosuke Yusa, Liqin Zhou, Nancy L. Craig, Matthew H. Wilson

Characterization of extended co-culture of non-typeable Haemophilus influenzae with primary human respiratory tissues

Non-typeable Haemophilus influenzae (NTHi) are human-adapted Gram-negative bacteria that comprise part of the normal flora of the human upper airway, but are also responsible for a number of mucosal infections such as otitis media and bronchitis....

Authors: Ren D, Nelson KL, Uchakin PN, Smith AL, Gu XX, Daines DA.

Phospholipid scramblase 1 mediates type i interferon-induced protection against staphylococcal a-toxin

The opportunistic gram-positive pathogen Staphylococcus aureus is a leading cause of pneumonia and sepsis. Staphylococcal a-toxin, a prototypical pore-forming toxin, is a major virulence factor of S. aureus clinical isolates, and lung epithelial...

Authors: Lizak M, Yarovinsky TO

High-efficiency transfection and survival rates of embryonic and adult mouse neural stem cells achieved by electroporation

Cells of the central nervous system are notoriously difficult to transfect. This is not only true for neurons and glial cells but also for dividing neural stem and progenitor cells (NSCs). About ten years ago a major advance was provided by...

Authors: Bettina Bertrama, Stefan Wiesea, Alexander von Holst

SST Peer Reviewed

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