Search Results

Membrane translocation of P-Rex1 is mediated by G protein beta gamma subunits and phosphoinositide 3-kinase

P-Rex1 is a guanine-nucleotide exchange factor (GEF) for the small GTPase Rac that is directly activated by the betagamma subunits of heterotrimeric G proteins and by the lipid second messenger phosphatidylinositol (3,4,5)-trisphosphate (PIP(3)),...

Authors: Barber MA, Donald S, Thelen S, Anderson KE, Thelen M, Welch HC

Characterization of a mutation in the PX domain of the NADPH oxidase component p40phox identifies a mechanism for negative regulation of superoxide production

The phagocyte oxidase (phox) protein p40phox contains a phox homology (PX) domain which, when expressed alone, interacts with phosphatidylinostol 3-phosphate (PtdIns(3)P). The functions of the PX domain in p40phox localization, association with the...

Authors: Chen J, He R, Minshall RD, Dinauer MC, Ye RD

Primary T-cells from human CD4/CCR5-transgenic rats support all early steps of HIV-1 replication including integration, but display impaired viral gene expression

BACKGROUND: In vivo studies on HIV-1 pathogenesis and testing of antiviral strategies have been hampered by the lack of an immunocompetent small animal model that is highly susceptible to HIV-1 infection. Since native rodents are non-permissive, we...

Authors: Goffinet C, Michel N, Allespach I, Tervo HM, Hermann V, Kräusslich HG, Greene WC, Keppler OT

Disease gene candidates revealed by expression profiling of retinal ganglion cell development

To what extent do postmitotic neurons regulate gene expression during development or after injury? We took advantage of our ability to highly purify retinal ganglion cells (RGCs) to profile their pattern of gene expression at 13 ages from embryonic...

Authors: Wang JT, Kunzevitzky NJ, Dugas JC, Cameron M, Barres BA, Goldberg JL

mRNA-Mediated Gene Delivery Into Human Progenitor Cells Promotes Highly Efficient Protein Expression

Gene transfer into human CD34+ haematopoietic progenitor cells (HPC) and multi-potent mesenchymal stromal cells (MSC) is an essential tool for numerous in vitro and in vivo applications including therapeutic strategies, such as tissue engineering and...

Authors: Wiehe JM, Ponsaerts P, Rojewski MT, Homann JM, Greiner J, Kronawitter D, Schrezenmeier H, Hombach V,...

SST Peer Reviewed

Localized activation of p21-activated kinase controls neuronal polarity and morphology

In the developing forebrain, neuronal polarization is a stepwise and initially reversible process that underlies correct migration and axon specification. Many aspects of cytoskeletal changes that accompany polarization are currently molecularly...

Authors: Jacobs T, Causeret F, Nishimura YV, Terao M, Norman A, Hoshino M, Nikolić M

Characterization of human immunodeficiency virus type 1 replication in immature and mature dendritic cells reveals dissociable cis- and trans-infection

Dendritic cells (DCs) transmit human immunodeficiency virus type 1 (HIV-1) to CD4(+) T cells through the trans- and cis-infection pathways; however, little is known about the relative efficiencies of these pathways and whether they are...

Authors: Dong C, Janas AM, Wang JH, Olson WJ, Wu L

Receptor-interacting protein 2 is a marker for resolution of peritoneal dialysis-associated peritonitis

There are no predictive factors for peritoneal dialysis-associated peritonitis; however, its resolution correlates with a cell-mediated Th1 immune response. We tested the hypothesis that induction of receptor-interacting protein 2 (RIP2), an assumed...

Authors: McCully ML, Baroja ML, Chau TA, Jain AK, Barra L, Salgado A, Blake PG, Madrenas J

An endoplasmic reticulum transmembrane prolyl 4-hydroxylase is induced by hypoxia and acts on HIF-alpha

Prolyl 4-hydroxylases (P4Hs) act on collagens (C-P4Hs) and the oxygen-dependent degradation domains (ODDDs) of HIF-alpha subunits (HIF-P4Hs) leading to degradation of the latter. We report data on a human P4H possessing a transmembrane domain...

Authors: Koivunen P, Tiainen P, Hyvärinen J, Williams KE, Sormunen R, Klaus SJ, Kivirikko KI, Myllyharju J

Basic Fibroblast Growth Factor Activates the MAPK and NFB Pathways That Converge on Elk-1 to Control Production of Matrix Metalloproteinase-13 by Human Adult Articular Chondrocytes

The pathology of joint destruction is associated with elevated production of basic fibroblast growth factor (bFGF) and matrix metalloproteinase-13 (MMP-13). In osteoarthritic joint disease, expression of bFGF and MMP-13 in chondrocytes and their...

Authors: Muddasani P, Norman JC, Ellman M, van Wijnen AJ, Im HJ

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