Data Type
Cell Information
(858)
Citations
(4968)
FAQ
(515)
Culture Media
(61)
Category
Transfection
(3843)
Primary Cells and Media
(1963)
Serum-free and Speciality Media
(1170)
Classical Media and Reagents
(1054)
Cell Lines and Primary Cancer Cells
(644)
Bioassay
(117)
Electrophoreses and Analysis
(99)
3D Cell Culture
(91)
Laboratory Instrumentation
(75)
Endotoxin Detection
(66)
Mycoplasma Detection and Prevention
(36)
Protozoa
(16)
Live Cell Imaging
(7)
Cell Services
(4)
Uncategorized
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
+ Show All
Research Area
Cancer Research/Cell Biology
(2175)
Basic Research
(1648)
Immunotherapy / Hematology
(1590)
Neurobiology
(617)
Stem Cells
(385)
Cardiovascular
(318)
Gene Expression
(278)
Dermatology/Tissue Engineering
(265)
Molecular Biology
(179)
Gastroenterology
(139)
Regenerative medicine
(96)
Drug Discovery
(94)
Endotoxin Testing
(90)
Toxicology
(81)
Respiratory Research
(71)
Parasitology
(69)
Uncategorized
(48)
+ Show All
Platform
Nucleofector® I/II/2b
(3039)
4D-Nucleofector® X-Unit
(596)
4D-Nucleofector® 96-well Systems
(245)
4D-Nucleofector® Y-Unit
(41)
384-well HT Nucleofector® System
(17)
4D-Nucleofector® LV-Unit
(13)
Topic
Signal transduction
(1269)
Microscopy of nucleofected cells
(747)
Cellular Function
(614)
Diseases (e.g. HIV)
(601)
Cell morphology/cytoskeleton
(405)
Apoptosis
(363)
Luciferase
(330)
Genome Editing
(312)
Intercellular interaction/communication
(293)
Differentiation of nucleofected cells
(230)
Expression of dominant-negative protein
(198)
Gene therapy
(180)
Hereditary diseases
(100)
Comparison to other transfection methods
(78)
Promoter analysis
(74)
RNA transfection
(57)
Antisense
(56)
Transplantation of nucleofected cells
(32)
Virus production
(19)
Infection of Nucleofected cells
(17)
Tissue transfection
(7)
Cryopreservation
(5)
+ Show All
Species
human
(524)
mouse
(183)
rat
(66)
monkey
(15)
bovine
(11)
hamster
(10)
unicellular
(9)
porcine
(8)
chicken
(7)
canine
(6)
rabbit
(3)
baboon
(2)
cell hybrid
(2)
fish
(2)
spodoptera frugiperda (army fall worm)
(2)
Trichopulsia ni (Cabbage Looper)
(1)
drosophila melanogaster (fruit fly)
(1)
equine
(1)
feline
(1)
ferret
(1)
potoroo
(1)
sheep
(1)
+ Show All
Characteristic
Adherent
(457)
Suspension
(400)
Format
Bullet Kit
(46)
Lonza Specialty Media
(14)
Classical Media
(1)
Origin
Animal
(42)
Non-Animal
(10)
6402 results sorted by
relevance
alphabetical
newest first
oldest first
Export Citations
I
w
a
n
t
t
o
s
t
i
m
u
l
a
t
e
H
u
m
a
n
T
c
e
l
l
s
p
o
s
t
N
u
c
l
e
o
f
e
c
t
i
o
n
®
.
A
r
e
t
h
e
r
e
a
n
y
p
r
e
c
a
u
t
i
o
n
s
t
o
k
e
e
p
i
n
m
i
n
d
?
After Nucleofection®, wait at least four hours before stimulation. Stimulating immediately after Nucleofection® may lead to increased cell mortality and poor activation of cells. For more details on the stimulation of Human T cells post...
M
y
N
u
c
l
e
o
f
e
c
t
o
r
®
S
o
l
u
t
i
o
n
w
a
s
f
r
o
z
e
n
.
I
s
t
h
i
s
a
p
r
o
b
l
e
m
?
As long as the supplement was not added to the Nucleofector® Solution, then there is no risk of any damage to the solutions. Even long-term storage of several months did not alter the performance of the Nucleofector® Solution. However, if the...
I
n
y
o
u
r
o
p
t
i
m
i
z
e
d
N
u
c
l
e
o
f
e
c
t
i
o
n
®
P
r
o
t
o
c
o
l
s
f
o
r
M
D
A
-
M
B
-
2
3
1
a
n
d
M
D
A
-
M
B
-
4
6
8
,
y
o
u
s
a
y
t
o
c
u
l
t
u
r
e
t
h
e
c
e
l
l
s
w
i
t
h
o
u
t
C
O
2
.
W
h
y
?
The media recommended for these cells is Leibovitz's L-15 medium which does not contain sodium bicarbonate to act as a buffer for the carbonic acid that would normally form in the presence of CO2. Furthermore, if the cells are cultured in Leibovitz's...
I
c
u
r
r
e
n
t
l
y
w
o
r
k
w
i
t
h
s
o
m
e
a
d
h
e
r
e
n
t
c
e
l
l
l
i
n
e
s
,
s
u
c
h
a
s
2
9
3
,
w
h
i
c
h
a
r
e
g
r
o
w
n
i
n
D
M
E
M
m
e
d
i
a
.
I
h
a
v
e
n
o
t
i
c
e
d
t
h
a
t
t
h
e
m
o
r
t
a
l
i
t
y
s
e
e
m
s
t
o
b
e
h
i
g
h
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
.
D
o
y
o
u
h
a
v
e
a
n
y
r
e
c
o
m
m
e
n
d
a
t
i
o
n
s
?
For some adherent cell lines we strongly recommend using RPMI instead of DMEM medium. As described in culture conditions of the cell line optimization kit protocol as well as the NIH3T3 (DSMZ) protocol, using RPMI instead of DMEM as "transfer and...
C
a
n
I
u
s
e
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
T
e
c
h
n
o
l
o
g
y
f
o
r
R
N
A
i
a
p
p
l
i
c
a
t
i
o
n
s
?
H
o
w
d
o
I
s
t
a
r
t
?
Yes. The Nucleofector® Technology can be easily applied for any RNAi substrate (siRNA, shRNA, miRNA). You can use the same conditions described in the cell type specific Optimized Protocol for DNA vectors (cDNA, shRNA or miRNA expressing plasmids) or...
D
o
y
o
u
h
a
v
e
a
n
y
s
i
R
N
A
N
u
c
l
e
o
f
e
c
t
i
o
n
®
r
e
s
u
l
t
s
u
s
i
n
g
c
o
n
c
e
n
t
r
a
t
i
o
n
s
l
o
w
e
r
t
h
a
n
5
0
n
M
?
Yes. Please click on the attached Technical Reference Guide: "Designing an RNAi Experiment Using Nucleofection®". On page 3 you can find a table with some examples.
C
a
n
I
o
r
d
e
r
a
n
y
o
f
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
K
i
t
c
o
m
p
o
n
e
n
t
s
s
e
p
a
r
a
t
e
l
y
?
No, we do not offer the components of the Nucleofector® Kits separately. The Nucleofection® Kits are only available as complete kits.
W
h
y
d
o
y
o
u
h
a
v
e
d
i
f
f
e
r
e
n
t
O
p
t
i
m
i
z
e
d
P
r
o
t
o
c
o
l
s
f
o
r
t
h
e
N
u
c
l
e
o
f
e
c
t
i
o
n
®
o
f
u
n
s
t
i
m
u
l
a
t
e
d
a
n
d
s
t
i
m
u
l
a
t
e
d
H
u
m
a
n
T
c
e
l
l
s
?
We found that the stimulation of T cells not only changes the cells' function but also significantly alters the requirements for successful Nucleofection®. That's why Lonza developed two Optimized Protocols for transfection giving different...
F
o
r
h
e
p
a
t
o
c
y
t
e
s
,
i
s
p
l
a
t
i
n
g
d
e
n
s
i
t
y
a
c
o
n
c
e
r
n
p
o
s
t
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
Yes, the optimized protocols for the standard Nucleofector® recommend the use of 6 well plates. Plating into a 12-well plate will pose a problem as less cells will attach at high densities.
I
s
i
t
p
o
s
s
i
b
l
e
t
o
u
s
e
f
r
o
z
e
n
p
r
i
m
a
r
y
C
D
3
4
+
h
e
m
a
t
o
p
o
i
e
t
i
c
s
t
e
m
c
e
l
l
s
f
o
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
Yes. For cryopreserved PBMCs or enriched CD34 populations, we recommend culturing the thawed cells 1-2 hours in culture medium before Nucleofection®. Any further enrichment procedure after thawing is not recommended. Viability of frozen nucleofected...
PREVIOUS
PAGE
335
PAGE
336
PAGE
337
PAGE
338
PAGE
339
PAGE
340
PAGE
341
PAGE
342
PAGE
343
NEXT