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Disease gene candidates revealed by expression profiling of retinal ganglion cell development

To what extent do postmitotic neurons regulate gene expression during development or after injury? We took advantage of our ability to highly purify retinal ganglion cells (RGCs) to profile their pattern of gene expression at 13 ages from embryonic...

Authors: Wang JT, Kunzevitzky NJ, Dugas JC, Cameron M, Barres BA, Goldberg JL

Transient transfection of DT40

Transient transfection of cell lines can be extremely useful in a number of applications. However, until recently, obtaining useful and reproducible percentages of transiently expressing DT40 was almost impossible. Standard techniques such as...

Authors: Franklin R, Sale JE

JAK2 tyrosine kinase phosphorylates PAK1 and regulates PAK1 activity and functions

The serine-threonine kinase PAK1 is activated by small GTPase-dependent and -independent mechanisms and promotes cell survival. However, the role of tyrosyl phosphorylation in the regulation of PAK1 function is poorly understood. In this study, we...

Authors: Rider L, Shatrova A, Feener EP, Webb L, Diakonova M

Decreased expression of Kruppel-like factors in memory B cells induces the rapid response typical of secondary antibody responses

Secondary antibody responses are characterized by the rapid kinetics of the responding cells, including the production of larger amounts of serum Ig compared with the primary response. Memory B cells, which are responsible for this phenomenon,...

Authors: Good KL, Tangye SG

Localized activation of p21-activated kinase controls neuronal polarity and morphology

In the developing forebrain, neuronal polarization is a stepwise and initially reversible process that underlies correct migration and axon specification. Many aspects of cytoskeletal changes that accompany polarization are currently molecularly...

Authors: Jacobs T, Causeret F, Nishimura YV, Terao M, Norman A, Hoshino M, Nikolić M

Robust CD4(+) and CD8(+) T cell responses to SIV using mRNA-transfected DC expressing autologous viral Ag

A potentially powerful strategy for therapeutic HIV vaccination is the use of DC transfected with mRNA encoding autologous viral Ag, as epitopes presented by transfected DC would exactly reflect those expressed by infected cells in the individual....

Authors: Melhem NM, Liu XD, Boczkowski D, Gilboa E, Barratt-Boyes SM

EP2 receptor mediates PGE2-induced cystogenesis of human renal epithelial cells

Autosomal-dominant polycystic kidney disease (ADPKD) is characterized by formation of cysts from tubular epithelial cells. Previous studies indicate that secretion of prostaglandin E(2) (PGE(2)) into cyst fluid and production of cAMP underlie cyst...

Authors: Elberg G, Elberg D, Lewis TV, Guruswamy S, Chen L, Logan CJ, Chan MD, Turman MA

Efficient and stable transgene expression in human embryonic stem cells using transposon-mediated gene transfer

Efficient and stable genetic modification of human embryonic stem (ES) cells is required to realize the full scientific and potential therapeutic use of these cells. Currently, only limited success toward this goal has been achieved without using a...

Authors: Wilber A, Linehan JL, Tian X, Woll PS, Morris JK, Belur LR, McIvor RS, Kaufman DS

Characterization of human immunodeficiency virus type 1 replication in immature and mature dendritic cells reveals dissociable cis- and trans-infection

Dendritic cells (DCs) transmit human immunodeficiency virus type 1 (HIV-1) to CD4(+) T cells through the trans- and cis-infection pathways; however, little is known about the relative efficiencies of these pathways and whether they are...

Authors: Dong C, Janas AM, Wang JH, Olson WJ, Wu L

Stimulation of c-Myc transcriptional activity by vIRF-3 of Kaposi's sarcoma-associated herpesvirus

Kaposi sarcoma-associated herpesvirus is associated with two lymphoproliferative disorders, primary effusion lymphoma (PEL) and Castleman disease. In PEL, Kaposi sarcoma-associated herpesvirus is present in a latent form expressing only few viral...

Authors: Lubyova B, Kellum MJ, Frisancho JA, Pitha PM

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