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What are the requirements for direct Nucleofection® of mRNA for protein expression?

The mRNA should be capped and polyadenylated. The conditions of Nucleofection® will be the same as for DNA with the particular cell type, i.e. follow the same protocol and use the same program, except one will likely need to add a much higher...
Missing: stable linesGuideline

Why am I not able to detect my fluorescent labeled siRNA (e.g. FITC) after 24 or 48 hours post-Nucleofection?

...analysis. Some labels are also subject to photo bleaching, pH changes, and may exhibit cell type specific effects as well. To minimize FITC-bleaching it may help to wrap the culture plates in aluminum foil immediately after Nucleofection™. So...
Missing: stable linesGuideline

Why can’t I obtain a Certificate of Analysis (COA) for a MycoAlert®/MycoAlert® PLUS/ToxiLight®/ViaLight® Kit via your Webpage-tool?

Lonza’s luminescent assays (including MycoAlert® Mycoplasma detection assay, MycoAlert® PLUS Mycoplasma detection assay, MycoAlert® Control set, ToxiLight® Non-Destructive Cytotoxicity Assay, and ViaLight® Plus Cell Proliferation...
Missing: stable linesGuideline

Do you have a protocol for the Nucleofection® of Plasmodium berghei using the Nucleofector® Systems

...strains with even better viability. They used program FF-167 in combination with P5 Primary Cell Solution. As the 96-well Shuttle™System also uses the conductive polymer electrodes, these conditions are transferable to the 4D-Nucleofector™ X Unit...
Missing: stable linesGuideline
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