Search Results

Cellular Notch responsiveness is defined by phosphoinositide 3-kinase-dependent signals

BACKGROUND: Notch plays a wide-ranging role in controlling cell fate, differentiation and development. The PI3K-Akt pathway is a similarly conserved signalling pathway which regulates processes such as differentiation, proliferation and survival....

Authors: McKenzie G, Ward G, Stallwood Y, Briend E, Papadia S, Lennard A, Turner M, Champion B, Hardingham...

Association of the Hermansky-Pudlak syndrome type-3 protein with clathrin

BACKGROUND: Hermansky-Pudlak syndrome (HPS) is a disorder of lysosome-related organelle biogenesis characterized by oculocutaneous albinism and prolonged bleeding. These clinical findings reflect defects in the formation of melanosomes in melanocytes...

Authors: Helip-Wooley A, Westbroek W, Dorward H, Mommaas M, Boissy RE, Gahl WA, Huizing M

The p16INK4a tumor suppressor controls p21WAF1 induction in response to ultraviolet light

p16(INK4a) and p21(WAF1), two major cyclin-dependent kinase inhibitors, are the products of two tumor suppressor genes that play important roles in various cellular metabolic pathways. p21(WAF1) is up-regulated in response to different DNA damaging...

Authors: Al-Mohanna MA, Al-Khalaf HH, Al-Yousef N, Aboussekhra A

Artefactual effects of lipid-based cell transfection reagents on AbetaPP processing and Abeta production

The study of amyloidogenic beta-amyloid precursor protein (AbetaPP) metabolism and amyloid beta protein (Abeta) production has been a major focus of Alzheimer's disease (AD) neuropathogenesis research. Cell transfection is a commonly employed method...

Authors: Romano DM, Dong Y, Hiltunen M, Tanzi RE, Xie Z

Regulation and function of glycogen synthase kinase-3 isoforms in neuronal survival

Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase consisting of two isoforms, alpha and beta. The activities of GSK-3 are regulated negatively by serine phosphorylation but positively by tyrosine phosphorylation. GSK-3 inactivation has...

Authors: Liang MH, Chuang DM

Protein-free transfection of CHO host cells with an IgGFusion protein-selection and characterisation of stable high producers and comparison to conventionally transfected clones

In order to improve the current techniques of cell cultivation in the absence of serum, we have developed a protein-free transfection protocol for CHO cells, based on the Nucleofectortrade mark technology. After starting with a heterogeneous pool of...

Authors: Lattenmayer C, Loeschel M, Schriebl K, Sterovsky T, Trummer E, Vorauer-Uhl K, Muller D, Katinger H,...

SST Peer Reviewed

Growth factor-dependent regulation of survivin by c-myc in human breast cancer

Survivin has emerged as a unique regulator of cell death through its response to growth factors, such as basic fibroblast growth factor (bFGF), which we have previously shown to be mitogen-activated protein kinase (MAPK) dependent. The...

Authors: Cosgrave N, Hill AD, Young LS

Structure function analysis of SH2D2A isoforms expressed in T cells reveals a crucial role for the proline rich region encoded by SH2D2A exon 7

BACKGROUND: The activation induced T cell specific adapter protein (TSAd), encoded by SH2D2A, interacts with and modulates Lck activity. Several transcript variants of TSAd mRNA exist, but their biological significance remains unknown. Here we...

Authors: Granum S, Sundvold-Gjerstad V, Dai KZ, Kolltveit KM, Hildebrand K, Huitfeldt HS, Lea T, Spurkland A

RNA-based gene therapy for Haemophilia B

Haemophilia B, a deficiency in clotting factor IX (FIX), occurs in about 1 in 25.000 males. Of these patients, approximately 40% are characterized as having 'severe' haemophilia (FIX below 1% of normal). Although the use of plasma-derived or rFIX has...

Authors: Peppelenbosch MP, Milano F, Ferreira CV, Knapisnka A, Diks SH, Spek A

The protective role of a small GTPase RhoE against UVB induced DNA damage in keratinocytes

The Rho family of GTPases regulates many aspects of cellular behavior through alterations to the actin cytoskeleton, acting as molecular switches cycling between the active, GTP-bound and the inactive, GDP-bound conformations. Unlike typical Rho...

Authors: Boswell SA, Ongusaha PP, Nghiem P, Lee SW

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