Search Results

Structurally distinct membrane nanotubes between human macrophages support long-distance vesicular traffic or surfing of bacteria

We report that two classes of membrane nanotubes between human monocyte-derived macrophages can be distinguished by their cytoskeletal structure and their functional properties. Thin membrane nanotubes contained only F-actin, whereas thicker...

Authors: Onfelt B, Nedvetzki S, Benninger RK, Purbhoo MA, Sowinski S, Hume AN, Seabra MC, Neil MA, French PM,...

Villin Severing Activity Enhances Actin-based Motility In Vivo

Villin, an actin-binding protein associated with the actin bundles that support microvilli, bundles, caps, nucleates, and severs actin in a calcium-dependant manner in vitro. We hypothesized that the severing activity of villin is responsible for its...

Authors: Revenu C, Courtois M, Michelot A, Sykes C, Louvard D, Robine S

The Rac Activator DOCK7 Regulates Neuronal Polarity through Local Phosphorylation of Stathmin/Op18

The polarization of a neuron generally results in the formation of one axon and multiple dendrites, allowing for the establishment of neuronal circuitry. The molecular mechanisms involved in priming one neurite to become the axon, particularly those...

Authors: Watabe-Uchida M, John KA, Janas JA, Newey SE, Van Aelst L

Interleukin-23 restores immunity to Mycobacterium tuberculosis infection in IL-12p40-deficient mice and is not required for the development of IL-17-secreting T cell responses

Mycobacterial Research Group, Centenary Institute of Cancer Medicine and Cell Biology, Newtown, New South Wales, Australia. Host control of Mycobacterium tuberculosis is dependent on the activation of CD4(+) T cells secreting IFN-gamma and their...

Authors: Wozniak TM, Ryan AA, Britton WJ

Glucose induces MafA expression in pancreatic beta cell lines via the hexosamine biosynthetic pathway

MafA is a basic leucine zipper transcription factor that regulates gene expression in both the neuroretina and pancreas. Within the pancreas, MafA is exclusively expressed in the beta cells and is involved in insulin gene transcription, insulin...

Authors: Vanderford NL, Andrali SS, Ozcan S

The Rab4a effector protein Rabip4 is involved in migration of NIH 3T3 fibroblasts

The small GTP-binding protein Rab4 has been involved in the recycling of alphavbeta3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration. In this study, we explored the...

Authors: Vukmirica J, Monzo P, Le Marchand-Brustel Y, Cormont M

Identification of class I MHC-associated phosphopeptides as targets for cancer immunotherapy

Alterations in phosphorylation of cellular proteins are a hallmark of malignant transformation. Degradation of these phosphoproteins could generate cancer-specific class I MHC-associated phosphopeptides recognizable by CD8(+) T lymphocytes. In a...

Authors: Zarling AL, Polefrone JM, Evans AM, Mikesh LM, Shabanowitz J, Lewis ST, Engelhard VH, Hunt DF

Cell divisions are required for L1 retrotransposition

LINE-1 (L1) retrotransposons comprise a large fraction of genomic DNAs of many organisms. Many L1 elements are active and may generate potentially deleterious mutations by inserting into genes, yet little is known about the control of...

Authors: Shi X, Seluanov A, Gorbunova V

Modification of the Pig CFTR Gene Mediated by Small Fragment Homologous Replacement

The generation of a pig model of cystic fibrosis (CF) is a multistep process. Initial steps in this process involved the design and cloning of a small DNA fragment (SDF) or large oligodeoxynucleotide (LODN) that contains the F508del mutation and a...

Authors: Maurisse R, Cheung J, Widdicombe J, Gruenert DC

Activation mechanism for CRAC current and store-operated Ca2+ entry: calcium influx factor and iPLA2beta -dependent pathway

Here we tested the role of calcium influx factor (CIF) and calcium-independent phospholipase A(2) (iPLA(2)) in activation of Ca(2+) release-activated Ca(2+) (CRAC) channels and store-operated Ca(2+) entry in rat basophilic leukemia (RBL-2H3) cells....

Authors: Csutora P, Zarayskiy V, Peter K, Monje F, Smani T, Zakharov SI, Litvinov D, Bolotina VM

Data Type

Category

Research Area

Platform

Topic

Species

Characteristic

Format

Origin